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. 1993 Feb;5(2):181–189. doi: 10.1105/tpc.5.2.181

Uncoupling Auxin and Ethylene Effects in Transgenic Tobacco and Arabidopsis Plants.

CP Romano 1, ML Cooper 1, HJ Klee 1
PMCID: PMC160261  PMID: 12271061

Abstract

Overproduction of auxin in transgenic plants also results in the overproduction of ethylene. Plants overproducing both auxin and ethylene display inhibition of stem elongation and growth, increased apical dominance, and leaf epinasty. To determine the relative roles of auxin and ethylene in these processes, transgenic tobacco and Arabidopsis plants expressing the auxin-overproducing tryptophan monooxygenase transgene were crossed to plants expressing an ethylene synthesis-inhibiting 1-aminocyclopropane-1-carboxylate deaminase transgene. Tobacco and Arabidopsis plants with elevated auxin and normal levels of ethylene were obtained by this strategy. Transgenic auxin-overproducing Arabidopsis plants were also crossed with the ethylene-insensitive ein1 and ein2 mutants. Analysis of these plants indicates that apical dominance and leaf epinasty are primarily controlled by auxin rather than ethylene. However, ethylene is partially responsible for the inhibition of stem elongation observed in auxin-overproducing tobacco. Finally, these data show that auxin overproduction can be effectively uncoupled from ethylene overproduction in transgenic plants to enable direct manipulation of plant morphology for agronomic and horticultural purposes.

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Selected References

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  1. A simple and general method for transferring genes into plants. Science. 1985 Mar 8;227(4691):1229–1231. doi: 10.1126/science.227.4691.1229. [DOI] [PubMed] [Google Scholar]
  2. Benfey P. N., Chua N. H. Regulated genes in transgenic plants. Science. 1989 Apr 14;244(4901):174–181. doi: 10.1126/science.244.4901.174. [DOI] [PubMed] [Google Scholar]
  3. Bleecker A. B., Estelle M. A., Somerville C., Kende H. Insensitivity to Ethylene Conferred by a Dominant Mutation in Arabidopsis thaliana. Science. 1988 Aug 26;241(4869):1086–1089. doi: 10.1126/science.241.4869.1086. [DOI] [PubMed] [Google Scholar]
  4. Burg S. P., Burg E. A. Ethylene formation in pea seedlings; its relation to the inhibition of bud growth caused by indole-3-acetic Acid. Plant Physiol. 1968 Jul;43(7):1069–1074. doi: 10.1104/pp.43.7.1069. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chen K. H., Miller A. N., Patterson G. W., Cohen J. D. A Rapid and Simple Procedure for Purification of Indole-3-Acetic Acid Prior to GC-SIM-MS Analysis. Plant Physiol. 1988 Mar;86(3):822–825. doi: 10.1104/pp.86.3.822. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jefferson R. A., Kavanagh T. A., Bevan M. W. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 1987 Dec 20;6(13):3901–3907. doi: 10.1002/j.1460-2075.1987.tb02730.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kay R., Chan A., Daly M., McPherson J. Duplication of CaMV 35S Promoter Sequences Creates a Strong Enhancer for Plant Genes. Science. 1987 Jun 5;236(4806):1299–1302. doi: 10.1126/science.236.4806.1299. [DOI] [PubMed] [Google Scholar]
  8. Kazemi S., Kefford N. P. Apical correlative effects in leaf epinasty of tomato. Plant Physiol. 1974 Oct;54(4):512–519. doi: 10.1104/pp.54.4.512. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Klee H. J., Hayford M. B., Kretzmer K. A., Barry G. F., Kishore G. M. Control of ethylene synthesis by expression of a bacterial enzyme in transgenic tomato plants. Plant Cell. 1991 Nov;3(11):1187–1193. doi: 10.1105/tpc.3.11.1187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kuyer P. P., Rosenbusch G., Yap S. H., de Boer H. H. Das variable Bild des Magenkarzinoids. Rofo. 1987 Feb;146(2):184–188. doi: 10.1055/s-2008-1048466. [DOI] [PubMed] [Google Scholar]
  11. Lincoln C., Britton J. H., Estelle M. Growth and development of the axr1 mutants of Arabidopsis. Plant Cell. 1990 Nov;2(11):1071–1080. doi: 10.1105/tpc.2.11.1071. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Medford J. I., Elmer J. S., Klee H. J. Molecular cloning and characterization of genes expressed in shoot apical meristems. Plant Cell. 1991 Apr;3(4):359–370. doi: 10.1105/tpc.3.4.359. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Reid M. S., Mor Y., Kofranek A. M. Epinasty of Poinsettias-the Role of Auxin and Ethylene. Plant Physiol. 1981 May;67(5):950–952. doi: 10.1104/pp.67.5.950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Richins R. D., Scholthof H. B., Shepherd R. J. Sequence of figwort mosaic virus DNA (caulimovirus group). Nucleic Acids Res. 1987 Oct 26;15(20):8451–8466. doi: 10.1093/nar/15.20.8451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Romano C. P., Hein M. B., Klee H. J. Inactivation of auxin in tobacco transformed with the indoleacetic acid-lysine synthetase gene of Pseudomonas savastanoi. Genes Dev. 1991 Mar;5(3):438–446. doi: 10.1101/gad.5.3.438. [DOI] [PubMed] [Google Scholar]
  16. Sanger M., Daubert S., Goodman R. M. Characteristics of a strong promoter from figwort mosaic virus: comparison with the analogous 35S promoter from cauliflower mosaic virus and the regulated mannopine synthase promoter. Plant Mol Biol. 1990 Mar;14(3):433–443. doi: 10.1007/BF00028779. [DOI] [PubMed] [Google Scholar]
  17. Sitbon F., Hennion S., Sundberg B., Little C. H., Olsson O., Sandberg G. Transgenic Tobacco Plants Coexpressing the Agrobacterium tumefaciens iaaM and iaaH Genes Display Altered Growth and Indoleacetic Acid Metabolism. Plant Physiol. 1992 Jul;99(3):1062–1069. doi: 10.1104/pp.99.3.1062. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ursin V. M., Bradford K. J. Auxin and Ethylene Regulation of Petiole Epinasty in Two Developmental Mutants of Tomato, diageotropica and Epinastic. Plant Physiol. 1989 Aug;90(4):1341–1346. doi: 10.1104/pp.90.4.1341. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Valvekens D., Van Montagu M., Van Lijsebettens M. Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection. Proc Natl Acad Sci U S A. 1988 Aug;85(15):5536–5540. doi: 10.1073/pnas.85.15.5536. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Yip W. K., Moore T., Yang S. F. Differential accumulation of transcripts for four tomato 1-aminocyclopropane-1-carboxylate synthase homologs under various conditions. Proc Natl Acad Sci U S A. 1992 Mar 15;89(6):2475–2479. doi: 10.1073/pnas.89.6.2475. [DOI] [PMC free article] [PubMed] [Google Scholar]

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