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. 1993 May;5(5):501–514. doi: 10.1105/tpc.5.5.501

A genetic analysis of DNA sequence requirements for Dissociation state I activity in tobacco.

J English 1, K Harrison 1, J D Jones 1
PMCID: PMC160288  PMID: 8390877

Abstract

Our objective was to test whether the double Ds structure correlated with Dissociation state I activity (i.e., high frequency of chromosome breakage and low frequency of reversion) in maize exhibited similar properties in tobacco. A genetic assay was established to test double Ds and related structures for their ability to cause loss of the linked marker genes streptomycin phosphotransferase and beta-glucuronidase in transgenic tobacco. An engineered double Ds element and a simple Ds element showed behavior consistent with that of state I and state II Ds elements, respectively, as described for maize. DNA structural rearrangements accompanied marker gene loss. Dissection of the double Ds structure showed that a left end and a right end of Ds in direct orientation were sufficient for the instability observed. This result suggested that left and right ends of Ds in direct orientation can participate in aberrant transposition events, consistent with two different models for double Ds-induced chromosome breakage proposed previously. Both models predict that the inversion of a half Ds element accompanies the aberrant transposition event. Such an inversion was detected by polymerase chain reaction experiments in tobacco and maize only when Activator activity was present in the genome.

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Selected References

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  1. A simple and general method for transferring genes into plants. Science. 1985 Mar 8;227(4691):1229–1231. doi: 10.1126/science.227.4691.1229. [DOI] [PubMed] [Google Scholar]
  2. Chen J., Greenblatt I. M., Dellaporta S. L. Transposition of Ac from the P locus of maize into unreplicated chromosomal sites. Genetics. 1987 Sep;117(1):109–116. doi: 10.1093/genetics/117.1.109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clausen R E, Cameron D R. Inheritance in Nicotiana Tabacum. Xviii. Monosomic Analysis. Genetics. 1944 Sep;29(5):447–477. doi: 10.1093/genetics/29.5.447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Courage-Tebbe U., Döring H. P., Fedoroff N., Starlinger P. The controlling element Ds at the Shrunken locus in Zea mays: structure of the unstable sh-m5933 allele and several revertants. Cell. 1983 Sep;34(2):383–393. doi: 10.1016/0092-8674(83)90372-0. [DOI] [PubMed] [Google Scholar]
  5. Dooner H. K., Belachew A. Chromosome breakage by pairs of closely linked transposable elements of the Ac-Ds family in maize. Genetics. 1991 Nov;129(3):855–862. doi: 10.1093/genetics/129.3.855. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dooner H. K., English J., Ralston E. J. The frequency of transposition of the maize element Activator is not affected by an adjacent deletion. Mol Gen Genet. 1988 Mar;211(3):485–491. doi: 10.1007/BF00425705. [DOI] [PubMed] [Google Scholar]
  7. Döring H. P., Nelsen-Salz B., Garber R., Tillmann E. Double Ds elements are involved in specific chromosome breakage. Mol Gen Genet. 1989 Oct;219(1-2):299–305. doi: 10.1007/BF00261191. [DOI] [PubMed] [Google Scholar]
  8. Döring H. P., Pahl I., Durany M. Chromosomal rearrangements caused by the aberrant transposition of double Ds elements are formed by Ds and adjacent non-Ds sequences. Mol Gen Genet. 1990 Oct;224(1):40–48. doi: 10.1007/BF00259449. [DOI] [PubMed] [Google Scholar]
  9. Döring H. P., Starlinger P. Barbara McClintock's controlling elements: now at the DNA level. Cell. 1984 Dec;39(2 Pt 1):253–259. doi: 10.1016/0092-8674(84)90002-3. [DOI] [PubMed] [Google Scholar]
  10. Fedoroff N., Wessler S., Shure M. Isolation of the transposable maize controlling elements Ac and Ds. Cell. 1983 Nov;35(1):235–242. doi: 10.1016/0092-8674(83)90226-x. [DOI] [PubMed] [Google Scholar]
  11. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  12. Geiser M., Weck E., Döring H. P., Werr W., Courage-Tebbe U., Tillmann E., Starlinger P. Genomic clones of a wild-type allele and a transposable element-induced mutant allele of the sucrose synthase gene of Zea mays L. EMBO J. 1982;1(11):1455–1460. doi: 10.1002/j.1460-2075.1982.tb01337.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Greenblatt I. M. A chromosome replication pattern deduced from pericarp phenotypes resulting from movements of the transposable element, modulator, in maize. Genetics. 1984 Oct;108(2):471–485. doi: 10.1093/genetics/108.2.471. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Greenblatt I. M. The mechanism of modulator transposition in maize. Genetics. 1968 Apr;58(4):585–597. doi: 10.1093/genetics/58.4.585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hobbs S. L., Kpodar P., DeLong C. M. The effect of T-DNA copy number, position and methylation on reporter gene expression in tobacco transformants. Plant Mol Biol. 1990 Dec;15(6):851–864. doi: 10.1007/BF00039425. [DOI] [PubMed] [Google Scholar]
  16. Jefferson R. A., Kavanagh T. A., Bevan M. W. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 1987 Dec 20;6(13):3901–3907. doi: 10.1002/j.1460-2075.1987.tb02730.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Jones J. D., Carland F. M., Maliga P., Dooner H. K. Visual detection of transposition of the maize element activator (ac) in tobacco seedlings. Science. 1989 Apr 14;244(4901):204–207. doi: 10.1126/science.244.4901.204. [DOI] [PubMed] [Google Scholar]
  18. Jones J. D., Carland F., Lim E., Ralston E., Dooner H. K. Preferential transposition of the maize element Activator to linked chromosomal locations in tobacco. Plant Cell. 1990 Aug;2(8):701–707. doi: 10.1105/tpc.2.8.701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Kunze R., Starlinger P. The putative transposase of transposable element Ac from Zea mays L. interacts with subterminal sequences of Ac. EMBO J. 1989 Nov;8(11):3177–3185. doi: 10.1002/j.1460-2075.1989.tb08476.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Matzke M. A., Primig M., Trnovsky J., Matzke A. J. Reversible methylation and inactivation of marker genes in sequentially transformed tobacco plants. EMBO J. 1989 Mar;8(3):643–649. doi: 10.1002/j.1460-2075.1989.tb03421.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. McClintock B. Induction of Instability at Selected Loci in Maize. Genetics. 1953 Nov;38(6):579–599. doi: 10.1093/genetics/38.6.579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Ralston E., English J., Dooner H. K. Chromosome-breaking structure in maize involving a fractured Ac element. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9451–9455. doi: 10.1073/pnas.86.23.9451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Velten J., Velten L., Hain R., Schell J. Isolation of a dual plant promoter fragment from the Ti plasmid of Agrobacterium tumefaciens. EMBO J. 1984 Dec 1;3(12):2723–2730. doi: 10.1002/j.1460-2075.1984.tb02202.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Weil C. F., Wessler S. R. Molecular evidence that chromosome breakage by Ds elements is caused by aberrant transposition. Plant Cell. 1993 May;5(5):515–522. doi: 10.1105/tpc.5.5.515. [DOI] [PMC free article] [PubMed] [Google Scholar]

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