Abstract
Arabidopsis fusca mutants display striking purple coloration due to anthocyanin accumulation in their cotyledons. We describe six recessive fusca mutants isolated from Agrobacterium-transformed Arabidopsis families. These mutants first become defective during embryogenesis and exhibit limited seedling development. Double mutant constructs revealed that developmental defects were not simply a consequence of anthocyanin accumulation. fusca seedlings showed altered responses to several environmental and endogenous factors. Allelism tests established that three fusca loci are represented by mutants previously described as defective in light-regulated responses. To study the molecular basis of the fusca phenotype, we cloned the FUS6 gene. FUS6 encodes a novel protein that is hydrophilic, alpha-helical, and contains potential protein kinase C phosphorylation sites. The FUSCA proteins appear to act in a network of signal transduction pathways critical for plant development.
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- Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403–410. doi: 10.1016/S0022-2836(05)80360-2. [DOI] [PubMed] [Google Scholar]
- Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
- Chory J. Out of darkness: mutants reveal pathways controlling light-regulated development in plants. Trends Genet. 1993 May;9(5):167–172. doi: 10.1016/0168-9525(93)90163-c. [DOI] [PubMed] [Google Scholar]
- Chory J., Peto C. A. Mutations in the DET1 gene affect cell-type-specific expression of light-regulated genes and chloroplast development in Arabidopsis. Proc Natl Acad Sci U S A. 1990 Nov;87(22):8776–8780. doi: 10.1073/pnas.87.22.8776. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chory J., Peto C., Feinbaum R., Pratt L., Ausubel F. Arabidopsis thaliana mutant that develops as a light-grown plant in the absence of light. Cell. 1989 Sep 8;58(5):991–999. doi: 10.1016/0092-8674(89)90950-1. [DOI] [PubMed] [Google Scholar]
- Deng X. W., Caspar T., Quail P. H. cop1: a regulatory locus involved in light-controlled development and gene expression in Arabidopsis. Genes Dev. 1991 Jul;5(7):1172–1182. doi: 10.1101/gad.5.7.1172. [DOI] [PubMed] [Google Scholar]
- Deng X. W., Matsui M., Wei N., Wagner D., Chu A. M., Feldmann K. A., Quail P. H. COP1, an Arabidopsis regulatory gene, encodes a protein with both a zinc-binding motif and a G beta homologous domain. Cell. 1992 Nov 27;71(5):791–801. doi: 10.1016/0092-8674(92)90555-q. [DOI] [PubMed] [Google Scholar]
- Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dominov J. A., Stenzler L., Lee S., Schwarz J. J., Leisner S., Howell S. H. Cytokinins and auxins control the expression of a gene in Nicotiana plumbaginifolia cells by feedback regulation. Plant Cell. 1992 Apr;4(4):451–461. doi: 10.1105/tpc.4.4.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dynlacht B. D., Weinzierl R. O., Admon A., Tjian R. The dTAFII80 subunit of Drosophila TFIID contains beta-transducin repeats. Nature. 1993 May 13;363(6425):176–179. doi: 10.1038/363176a0. [DOI] [PubMed] [Google Scholar]
- Francis F. J. Food colorants: anthocyanins. Crit Rev Food Sci Nutr. 1989;28(4):273–314. doi: 10.1080/10408398909527503. [DOI] [PubMed] [Google Scholar]
- Fritze K., Staiger D., Czaja I., Walden R., Schell J., Wing D. Developmental and UV Light Regulation of the Snapdragon Chalcone Synthase Promoter. Plant Cell. 1991 Sep;3(9):893–905. doi: 10.1105/tpc.3.9.893. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gubler U., Hoffman B. J. A simple and very efficient method for generating cDNA libraries. Gene. 1983 Nov;25(2-3):263–269. doi: 10.1016/0378-1119(83)90230-5. [DOI] [PubMed] [Google Scholar]
- Kieber J. J., Rothenberg M., Roman G., Feldmann K. A., Ecker J. R. CTR1, a negative regulator of the ethylene response pathway in Arabidopsis, encodes a member of the raf family of protein kinases. Cell. 1993 Feb 12;72(3):427–441. doi: 10.1016/0092-8674(93)90119-b. [DOI] [PubMed] [Google Scholar]
- Kubasek W. L., Shirley B. W., McKillop A., Goodman H. M., Briggs W., Ausubel F. M. Regulation of Flavonoid Biosynthetic Genes in Germinating Arabidopsis Seedlings. Plant Cell. 1992 Oct;4(10):1229–1236. doi: 10.1105/tpc.4.10.1229. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lloyd A. M., Walbot V., Davis R. W. Arabidopsis and Nicotiana anthocyanin production activated by maize regulators R and C1. Science. 1992 Dec 11;258(5089):1773–1775. doi: 10.1126/science.1465611. [DOI] [PubMed] [Google Scholar]
- Marx J. Forging a path to the nucleus. Science. 1993 Jun 11;260(5114):1588–1590. doi: 10.1126/science.8503004. [DOI] [PubMed] [Google Scholar]
- Medford J. I., Behringer F. J., Callos J. D., Feldmann K. A. Normal and Abnormal Development in the Arabidopsis Vegetative Shoot Apex. Plant Cell. 1992 Jun;4(6):631–643. doi: 10.1105/tpc.4.6.631. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meinke D. W. A Homoeotic Mutant of Arabidopsis thaliana with Leafy Cotyledons. Science. 1992 Dec 4;258(5088):1647–1650. doi: 10.1126/science.258.5088.1647. [DOI] [PubMed] [Google Scholar]
- Meinke D. W. Perspectives on Genetic Analysis of Plant Embryogenesis. Plant Cell. 1991 Sep;3(9):857–866. doi: 10.1105/tpc.3.9.857. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nicholas C. D., Lindstrom J. T., Vodkin L. O. Variation of proline rich cell wall proteins in soybean lines with anthocyanin mutations. Plant Mol Biol. 1993 Jan;21(1):145–156. doi: 10.1007/BF00039625. [DOI] [PubMed] [Google Scholar]
- Okada K., Shimura Y. Aspects of recent developments in mutational studies of plant signaling pathways. Cell. 1992 Aug 7;70(3):369–372. doi: 10.1016/0092-8674(92)90159-a. [DOI] [PubMed] [Google Scholar]
- Sung Z. R., Belachew A., Shunong B., Bertrand-Garcia R. EMF, an Arabidopsis Gene Required for Vegetative Shoot Development. Science. 1992 Dec 4;258(5088):1645–1647. doi: 10.1126/science.258.5088.1645. [DOI] [PubMed] [Google Scholar]
- Weiss D., van Tunen A. J., Halevy A. H., Mol J. N., Gerats A. G. Stamens and Gibberellic Acid in the Regulation of Flavonoid Gene Expression in the Corolla of Petunia hybrida. Plant Physiol. 1990 Oct;94(2):511–515. doi: 10.1104/pp.94.2.511. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wingender R., Röhrig H., Höricke C., Schell J. cis-regulatory elements involved in ultraviolet light regulation and plant defense. Plant Cell. 1990 Oct;2(10):1019–1026. doi: 10.1105/tpc.2.10.1019. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Woodgett J. R., Gould K. L., Hunter T. Substrate specificity of protein kinase C. Use of synthetic peptides corresponding to physiological sites as probes for substrate recognition requirements. Eur J Biochem. 1986 Nov 17;161(1):177–184. doi: 10.1111/j.1432-1033.1986.tb10139.x. [DOI] [PubMed] [Google Scholar]
- Yeung E. C., Law S. K. Serial sectioning techniques for a modified LKB Historesin. Stain Technol. 1987 May;62(3):147–153. doi: 10.3109/10520298709107985. [DOI] [PubMed] [Google Scholar]