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. 1994 Jul;6(7):935–945. doi: 10.1105/tpc.6.7.935

Cutinase gene disruption in Fusarium solani f sp pisi decreases its virulence on pea.

L M Rogers 1, M A Flaishman 1, P E Kolattukudy 1
PMCID: PMC160490  PMID: 8069105

Abstract

Fusarium solani f sp pisi (Nectria haematococca) isolate 77-2-3 with one cutinase gene produced 10 to 20% of the cutinase produced by isolate T-8 that has multiple cutinase genes, whereas cutinase gene-disrupted mutant 77-102 of isolate 77-2-3 did not produce cutinase. On the surface of pea stem segments, lesion formation was most frequent and most severe with T-8, less frequent and less severe with 77-2-3, and much less frequent and much milder with the gene-disrupted mutant. Microscopic examination of the lesions caused by the mutant strongly suggest that it penetrated the host mostly via the stomata. In seedling assays, 77-2-3 caused severe lesions on every seedling and stunted growth, whereas the mutant showed very mild lesions on one-third of the seedlings with no stunting. Thus, cutinase gene disruption resulted in a significant decrease in the pathogenicity of F. s. pisi on pea.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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