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. 1994 Aug;6(8):1065–1076. doi: 10.1105/tpc.6.8.1065

Roles of the GLABROUS1 and TRANSPARENT TESTA GLABRA Genes in Arabidopsis Trichome Development.

J C Larkin 1, D G Oppenheimer 1, A M Lloyd 1, E T Paparozzi 1, M D Marks 1
PMCID: PMC160501  PMID: 12244266

Abstract

Arabidopsis trichomes are branched, single-celled epidermal hairs. These specialized cells provide a convenient model for investigating the specification of cell fate in plants. Two key genes regulating the initiation of trichome development are GLABROUS1 (GL1) and TRANSPARENT TESTA GLABRA (TTG). GL1 is a member of the myb gene family. The maize R gene, which can functionally complement the Arabidopsis ttg mutation, encodes a basic helix-loop-helix protein. We used constitutively expressed copies of the GL1 and R genes to test hypotheses about the roles of GL1 and TTG in trichome development. The results support the hypothesis that TTG and GL1 cooperate at the same point in the trichome developmental pathway. Furthermore, the constitutive expression of both GL1 and R in the same plant caused trichomes to develop on all shoot epidermal surfaces. Results were also obtained indicating that TTG plays an additional role in inhibiting neighboring cells from becoming trichomes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Esch J. J., Oppenheimer D. G., Marks M. D. Characterization of a weak allele of the GL1 gene of Arabidopsis thaliana. Plant Mol Biol. 1994 Jan;24(1):203–207. doi: 10.1007/BF00040586. [DOI] [PubMed] [Google Scholar]
  2. Fehon R. G., Kooh P. J., Rebay I., Regan C. L., Xu T., Muskavitch M. A., Artavanis-Tsakonas S. Molecular interactions between the protein products of the neurogenic loci Notch and Delta, two EGF-homologous genes in Drosophila. Cell. 1990 May 4;61(3):523–534. doi: 10.1016/0092-8674(90)90534-l. [DOI] [PubMed] [Google Scholar]
  3. Ghysen A., Dambly-Chaudière C., Jan L. Y., Jan Y. N. Cell interactions and gene interactions in peripheral neurogenesis. Genes Dev. 1993 May;7(5):723–733. doi: 10.1101/gad.7.5.723. [DOI] [PubMed] [Google Scholar]
  4. Gill G., Ptashne M. Negative effect of the transcriptional activator GAL4. Nature. 1988 Aug 25;334(6184):721–724. doi: 10.1038/334721a0. [DOI] [PubMed] [Google Scholar]
  5. Goff S. A., Cone K. C., Chandler V. L. Functional analysis of the transcriptional activator encoded by the maize B gene: evidence for a direct functional interaction between two classes of regulatory proteins. Genes Dev. 1992 May;6(5):864–875. doi: 10.1101/gad.6.5.864. [DOI] [PubMed] [Google Scholar]
  6. Herman P. L., Marks M. D. Trichome Development in Arabidopsis thaliana. II. Isolation and Complementation of the GLABROUS1 Gene. Plant Cell. 1989 Nov;1(11):1051–1055. doi: 10.1105/tpc.1.11.1051. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hülskamp M., Misŕa S., Jürgens G. Genetic dissection of trichome cell development in Arabidopsis. Cell. 1994 Feb 11;76(3):555–566. doi: 10.1016/0092-8674(94)90118-x. [DOI] [PubMed] [Google Scholar]
  8. Klein T. M., Roth B. A., Fromm M. E. Regulation of anthocyanin biosynthetic genes introduced into intact maize tissues by microprojectiles. Proc Natl Acad Sci U S A. 1989 Sep;86(17):6681–6685. doi: 10.1073/pnas.86.17.6681. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Koornneef M., Dellaert L. W., van der Veen J. H. EMS- and radiation-induced mutation frequencies at individual loci in Arabidopsis thaliana (L.) Heynh. Mutat Res. 1982 Mar;93(1):109–123. doi: 10.1016/0027-5107(82)90129-4. [DOI] [PubMed] [Google Scholar]
  10. Larkin J. C., Oppenheimer D. G., Marks M. D. The GL1 gene and the trichome developmental pathway in Arabidopsis thaliana. Results Probl Cell Differ. 1994;20:259–275. doi: 10.1007/978-3-540-48037-2_12. [DOI] [PubMed] [Google Scholar]
  11. Larkin J. C., Oppenheimer D. G., Pollock S., Marks M. D. Arabidopsis GLABROUS1 Gene Requires Downstream Sequences for Function. Plant Cell. 1993 Dec;5(12):1739–1748. doi: 10.1105/tpc.5.12.1739. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lloyd A. M., Walbot V., Davis R. W. Arabidopsis and Nicotiana anthocyanin production activated by maize regulators R and C1. Science. 1992 Dec 11;258(5089):1773–1775. doi: 10.1126/science.1465611. [DOI] [PubMed] [Google Scholar]
  13. Ludwig S. R., Habera L. F., Dellaporta S. L., Wessler S. R. Lc, a member of the maize R gene family responsible for tissue-specific anthocyanin production, encodes a protein similar to transcriptional activators and contains the myc-homology region. Proc Natl Acad Sci U S A. 1989 Sep;86(18):7092–7096. doi: 10.1073/pnas.86.18.7092. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Marks M. D., Feldmann K. A. Trichome Development in Arabidopsis thaliana. I. T-DNA Tagging of the GLABROUS1 Gene. Plant Cell. 1989 Nov;1(11):1043–1050. doi: 10.1105/tpc.1.11.1043. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Meinke D. W. A Homoeotic Mutant of Arabidopsis thaliana with Leafy Cotyledons. Science. 1992 Dec 4;258(5088):1647–1650. doi: 10.1126/science.258.5088.1647. [DOI] [PubMed] [Google Scholar]
  16. Mizukami Y., Ma H. Ectopic expression of the floral homeotic gene AGAMOUS in transgenic Arabidopsis plants alters floral organ identity. Cell. 1992 Oct 2;71(1):119–131. doi: 10.1016/0092-8674(92)90271-d. [DOI] [PubMed] [Google Scholar]
  17. Oppenheimer D. G., Herman P. L., Sivakumaran S., Esch J., Marks M. D. A myb gene required for leaf trichome differentiation in Arabidopsis is expressed in stipules. Cell. 1991 Nov 1;67(3):483–493. doi: 10.1016/0092-8674(91)90523-2. [DOI] [PubMed] [Google Scholar]
  18. Paz-Ares J., Ghosal D., Wienand U., Peterson P. A., Saedler H. The regulatory c1 locus of Zea mays encodes a protein with homology to myb proto-oncogene products and with structural similarities to transcriptional activators. EMBO J. 1987 Dec 1;6(12):3553–3558. doi: 10.1002/j.1460-2075.1987.tb02684.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. van der Krol A. R., Mur L. A., Beld M., Mol J. N., Stuitje A. R. Flavonoid genes in petunia: addition of a limited number of gene copies may lead to a suppression of gene expression. Plant Cell. 1990 Apr;2(4):291–299. doi: 10.1105/tpc.2.4.291. [DOI] [PMC free article] [PubMed] [Google Scholar]

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