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. 1994 Dec;6(12):1703–1712. doi: 10.1105/tpc.6.12.1703

Function of Oxidative Cross-Linking of Cell Wall Structural Proteins in Plant Disease Resistance.

L F Brisson 1, R Tenhaken 1, C Lamb 1
PMCID: PMC160556  PMID: 12244231

Abstract

Elicitation of soybean cells causes a rapid insolubilization of two cell wall structural proteins, p33 and p100. Likewise, a short elicitation of 30 min rendered cell walls more refractory to enzyme digestion as assayed by the yield of protoplasts released. This effect could be ascribed to protein cross-linking because of its insensitivity to inhibitors of transcription (actinomycin D) and translation (cycloheximide) and its induction by exogenous H2O2. Moreover, the induced loss of protoplasts could be prevented by preincubation with DTT, which also blocks peroxidase-mediated oxidative cross-linking. The operation of protein insolubilization in plant defense was also demonstrated by its occurrence in the incompatible interaction but not in the compatible interaction between soybean and Pseudomonas syringae pv glycinea. Likewise, protein insolubilization was observed in bean during non-host hypersensitive resistance to the tobacco pathogen P. s. pv tabaci mediated by the hypersensitive resistance and pathogenicity (Hrp) gene cluster. Our data strongly suggest that rapid protein insolubilization leads to a strengthened cell wall, and this mechanism functions as a rapid defense in the initial stages of the hypersensitive response prior to deployment of transcription-dependent defenses.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Apostol I., Heinstein P. F., Low P. S. Rapid Stimulation of an Oxidative Burst during Elicitation of Cultured Plant Cells : Role in Defense and Signal Transduction. Plant Physiol. 1989 May;90(1):109–116. doi: 10.1104/pp.90.1.109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ayers A. R., Ebel J., Valent B., Albersheim P. Host-Pathogen Interactions: X. Fractionation and Biological Activity of an Elicitor Isolated from the Mycelial Walls of Phytophthora megasperma var. sojae. Plant Physiol. 1976 May;57(5):760–765. doi: 10.1104/pp.57.5.760. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baggiolini M., Wymann M. P. Turning on the respiratory burst. Trends Biochem Sci. 1990 Feb;15(2):69–72. doi: 10.1016/0968-0004(90)90179-f. [DOI] [PubMed] [Google Scholar]
  4. Bowles D. J. Defense-related proteins in higher plants. Annu Rev Biochem. 1990;59:873–907. doi: 10.1146/annurev.bi.59.070190.004301. [DOI] [PubMed] [Google Scholar]
  5. Bradley D. J., Kjellbom P., Lamb C. J. Elicitor- and wound-induced oxidative cross-linking of a proline-rich plant cell wall protein: a novel, rapid defense response. Cell. 1992 Jul 10;70(1):21–30. doi: 10.1016/0092-8674(92)90530-p. [DOI] [PubMed] [Google Scholar]
  6. Church G. M., Gilbert W. Genomic sequencing. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991–1995. doi: 10.1073/pnas.81.7.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dixon R. A., Harrison M. J. Activation, structure, and organization of genes involved in microbial defense in plants. Adv Genet. 1990;28:165–234. doi: 10.1016/s0065-2660(08)60527-1. [DOI] [PubMed] [Google Scholar]
  8. Hedrick S. A., Bell J. N., Boller T., Lamb C. J. Chitinase cDNA cloning and mRNA induction by fungal elicitor, wounding, and infection. Plant Physiol. 1988 Jan;86(1):182–186. doi: 10.1104/pp.86.1.182. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hockenbery D. M., Oltvai Z. N., Yin X. M., Milliman C. L., Korsmeyer S. J. Bcl-2 functions in an antioxidant pathway to prevent apoptosis. Cell. 1993 Oct 22;75(2):241–251. doi: 10.1016/0092-8674(93)80066-n. [DOI] [PubMed] [Google Scholar]
  10. Hong J. C., Nagao R. T., Key J. L. Developmentally regulated expression of soybean proline-rich cell wall protein genes. Plant Cell. 1989 Sep;1(9):937–943. doi: 10.1105/tpc.1.9.937. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ishii S. Factors Influencing Protoplast Viability of Suspension-Cultured Rice Cells during Isolation Process. Plant Physiol. 1988 Sep;88(1):26–29. doi: 10.1104/pp.88.1.26. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jakobek J. L., Lindgren P. B. Generalized Induction of Defense Responses in Bean Is Not Correlated with the Induction of the Hypersensitive Reaction. Plant Cell. 1993 Jan;5(1):49–56. doi: 10.1105/tpc.5.1.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lamb C. J., Lawton M. A., Dron M., Dixon R. A. Signals and transduction mechanisms for activation of plant defenses against microbial attack. Cell. 1989 Jan 27;56(2):215–224. doi: 10.1016/0092-8674(89)90894-5. [DOI] [PubMed] [Google Scholar]
  14. Lawton M. A., Lamb C. J. Transcriptional activation of plant defense genes by fungal elicitor, wounding, and infection. Mol Cell Biol. 1987 Jan;7(1):335–341. doi: 10.1128/mcb.7.1.335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Meier B. M., Shaw N., Slusarenko A. J. Spatial and temporal accumulation of defense gene transcripts in bean (Phaseolus vulgaris) leaves in relation to bacteria-induced hypersensitive cell death. Mol Plant Microbe Interact. 1993 Jul-Aug;6(4):453–466. doi: 10.1094/mpmi-6-453. [DOI] [PubMed] [Google Scholar]
  16. Smith P. K., Krohn R. I., Hermanson G. T., Mallia A. K., Gartner F. H., Provenzano M. D., Fujimoto E. K., Goeke N. M., Olson B. J., Klenk D. C. Measurement of protein using bicinchoninic acid. Anal Biochem. 1985 Oct;150(1):76–85. doi: 10.1016/0003-2697(85)90442-7. [DOI] [PubMed] [Google Scholar]
  17. Staskawicz B., Dahlbeck D., Keen N., Napoli C. Molecular characterization of cloned avirulence genes from race 0 and race 1 of Pseudomonas syringae pv. glycinea. J Bacteriol. 1987 Dec;169(12):5789–5794. doi: 10.1128/jb.169.12.5789-5794.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Walker-Simmons M., Holländer-Czytko H., Andersen J. K., Ryan C. A. Wound signals in plants: A systemic plant wound signal alters plasma membrane integrity. Proc Natl Acad Sci U S A. 1984 Jun;81(12):3737–3741. doi: 10.1073/pnas.81.12.3737. [DOI] [PMC free article] [PubMed] [Google Scholar]

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