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. 1993 Feb;101(2):373–383. doi: 10.1104/pp.101.2.373

Tobacco and Parsley 4-Coumarate:Coenzyme A Ligase Genes Are Temporally and Spatially Regulated in a Cell Type-Specific Manner during Tobacco Flower Development.

S Reinold 1, K D Hauffe 1, C J Douglas 1
PMCID: PMC160581  PMID: 12231692

Abstract

The enzyme 4-coumarate:coenzyme A ligase (4CL) plays a key role in phenylpropanoid metabolism by supplying the precursors (coenzyme A esters of cinnamic acid derivatives) used for the biosynthesis of diverse natural products, many of which play functional roles in floral organs. In this study, we used in situ hybridization and histochemical localization of [beta]-glucuronidase (GUS) activity to define in detail the temporal and spatial patterns of 4CL-1 expression during tobacco flower development. Sectioned flowers from tobacco plants transgenic for a complete copy of the parsley (Petroselinum crispum) 4CL-1 gene were hybridized to probes that distinguished between 4CL-1 transcripts and endogenous tobacco 4CL transcripts. Both probes hybridized with similar cell type-specific patterns to carpels, anthers, petals, and sepals, and the sites of hybridization varied during flower development. The sites of hybridization generally coincided temporally and spatially with sites of 4CL-GUS expression, suggesting that most of the expression patterns are regulated by 4CL-1 promoter sequences, but lack of correlation between sites of 4CL mRNA accumulation and GUS activity in portions of the petal suggest that downstream sequences may mediate some aspects of developmentally regulated 4CL-1 expression. These results indicate that the introduced 4CL-1 gene correctly responds to endogenous tobacco developmental signals and demonstrate complex temporal and spatial patterns of expression during floral organ differentiation.

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Selected References

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  1. Becker-André M., Schulze-Lefert P., Hahlbrock K. Structural comparison, modes of expression, and putative cis-acting elements of the two 4-coumarate: CoA ligase genes in potato. J Biol Chem. 1991 May 5;266(13):8551–8559. [PubMed] [Google Scholar]
  2. Bevan M., Shufflebottom D., Edwards K., Jefferson R., Schuch W. Tissue- and cell-specific activity of a phenylalanine ammonia-lyase promoter in transgenic plants. EMBO J. 1989 Jul;8(7):1899–1906. doi: 10.1002/j.1460-2075.1989.tb03592.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bowman J. L., Drews G. N., Meyerowitz E. M. Expression of the Arabidopsis floral homeotic gene AGAMOUS is restricted to specific cell types late in flower development. Plant Cell. 1991 Aug;3(8):749–758. doi: 10.1105/tpc.3.8.749. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Coen E. S., Meyerowitz E. M. The war of the whorls: genetic interactions controlling flower development. Nature. 1991 Sep 5;353(6339):31–37. doi: 10.1038/353031a0. [DOI] [PubMed] [Google Scholar]
  5. Dooner H. K., Robbins T. P., Jorgensen R. A. Genetic and developmental control of anthocyanin biosynthesis. Annu Rev Genet. 1991;25:173–199. doi: 10.1146/annurev.ge.25.120191.001133. [DOI] [PubMed] [Google Scholar]
  6. Douglas C. J., Hauffe K. D., Ites-Morales M. E., Ellard M., Paszkowski U., Hahlbrock K., Dangl J. L. Exonic sequences are required for elicitor and light activation of a plant defense gene, but promoter sequences are sufficient for tissue specific expression. EMBO J. 1991 Jul;10(7):1767–1775. doi: 10.1002/j.1460-2075.1991.tb07701.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Douglas C., Hoffmann H., Schulz W., Hahlbrock K. Structure and elicitor or u.v.-light-stimulated expression of two 4-coumarate:CoA ligase genes in parsley. EMBO J. 1987 May;6(5):1189–1195. doi: 10.1002/j.1460-2075.1987.tb02353.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Goldberg R. B. Plants: novel developmental processes. Science. 1988 Jun 10;240(4858):1460–1467. doi: 10.1126/science.3287622. [DOI] [PubMed] [Google Scholar]
  9. Hauffe K. D., Paszkowski U., Schulze-Lefert P., Hahlbrock K., Dangl J. L., Douglas C. J. A parsley 4CL-1 promoter fragment specifies complex expression patterns in transgenic tobacco. Plant Cell. 1991 May;3(5):435–443. doi: 10.1105/tpc.3.5.435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jackson D., Culianez-Macia F., Prescott A. G., Roberts K., Martin C. Expression patterns of myb genes from Antirrhinum flowers. Plant Cell. 1991 Feb;3(2):115–125. doi: 10.1105/tpc.3.2.115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Koes R. E., Van Blokland R., Quattrocchio F., Van Tunen A. J., Mol JNM. Chalcone Synthase Promoters in Petunia Are Active in Pigmented and Unpigmented Cell Types. Plant Cell. 1990 May;2(5):379–392. doi: 10.1105/tpc.2.5.379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Koltunow A. M., Truettner J., Cox K. H., Wallroth M., Goldberg R. B. Different Temporal and Spatial Gene Expression Patterns Occur during Anther Development. Plant Cell. 1990 Dec;2(12):1201–1224. doi: 10.1105/tpc.2.12.1201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Liang X. W., Dron M., Schmid J., Dixon R. A., Lamb C. J. Developmental and environmental regulation of a phenylalanine ammonia-lyase-beta-glucuronidase gene fusion in transgenic tobacco plants. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9284–9288. doi: 10.1073/pnas.86.23.9284. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lozoya E., Hoffmann H., Douglas C., Schulz W., Scheel D., Hahlbrock K. Primary structures and catalytic properties of isoenzymes encoded by the two 4-coumarate: CoA ligase genes in parsley. Eur J Biochem. 1988 Oct 1;176(3):661–667. doi: 10.1111/j.1432-1033.1988.tb14328.x. [DOI] [PubMed] [Google Scholar]
  15. Ma H., Yanofsky M. F., Meyerowitz E. M. AGL1-AGL6, an Arabidopsis gene family with similarity to floral homeotic and transcription factor genes. Genes Dev. 1991 Mar;5(3):484–495. doi: 10.1101/gad.5.3.484. [DOI] [PubMed] [Google Scholar]
  16. Ohl S., Hedrick S. A., Chory J., Lamb C. J. Functional properties of a phenylalanine ammonia-lyase promoter from Arabidopsis. Plant Cell. 1990 Sep;2(9):837–848. doi: 10.1105/tpc.2.9.837. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schmelzer E., Jahnen W., Hahlbrock K. In situ localization of light-induced chalcone synthase mRNA, chalcone synthase, and flavonoid end products in epidermal cells of parsley leaves. Proc Natl Acad Sci U S A. 1988 May;85(9):2989–2993. doi: 10.1073/pnas.85.9.2989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schmelzer E., Kruger-Lebus S., Hahlbrock K. Temporal and Spatial Patterns of Gene Expression around Sites of Attempted Fungal Infection in Parsley Leaves. Plant Cell. 1989 Oct;1(10):993–1001. doi: 10.1105/tpc.1.10.993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schmid J., Doerner P. W., Clouse S. D., Dixon R. A., Lamb C. J. Developmental and environmental regulation of a bean chalcone synthase promoter in transgenic tobacco. Plant Cell. 1990 Jul;2(7):619–631. doi: 10.1105/tpc.2.7.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. van der Meer I. M., Stam M. E., van Tunen A. J., Mol J. N., Stuitje A. R. Antisense inhibition of flavonoid biosynthesis in petunia anthers results in male sterility. Plant Cell. 1992 Mar;4(3):253–262. doi: 10.1105/tpc.4.3.253. [DOI] [PMC free article] [PubMed] [Google Scholar]

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