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. 1993 Feb;101(2):407–414. doi: 10.1104/pp.101.2.407

Hypoxic and Anoxic Induction of Alcohol Dehydrogenase in Roots and Shoots of Seedlings of Zea mays (Adh Transcripts and Enzyme Activity).

D L Andrews 1, B G Cobb 1, J R Johnson 1, M C Drew 1
PMCID: PMC160585  PMID: 12231696

Abstract

Alcohol dehydrogenase (ADH) is one of a number of enzymes of glycolysis and fermentation known to be synthesized preferentially under low O2 conditions. We examined levels of Adh1 transcripts and of ADH activity in 5-mm root tips, root axes (the remainder of the seminal root), and shoots of maize (Zea mays L. cv TX 5855) seedlings. Seedlings with roots averaging about 60-mm long were transferred from fully aerobic conditions (solutions sparged with 40% [v/v] O2) to anaerobic (O2-free) conditions, or to an intermediate O2 concentration. There was no prior acclimation to low O2. In root tips, anoxia induced Adh1 transcripts and enzyme activity at 6 h, but this was followed by a rapid decline so that at 12 to 18 h neither were detectable and the root tips were dead. In contrast, higher levels of Adh1 transcripts and enzyme activity were maintained for at least 48 h in root axes and shoots. When induction at 6 h was measured over a wide range of O2 concentrations, a peak in ADH activity occurred in all tissues at 4% (v/v) O2. Maximum levels of transcripts, however, were in the range of 0 to 4% O2, depending on the tissue. The time course of hypoxic induction (at 4% O2) in root tips showed a peak in transcript levels at 6 h, whereas ADH activity continued to rise throughout the 24-h experiment. These results show that in root tips, ADH induction by anoxia was small and transient relative to induction by hypoxia.

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Selected References

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  1. Bailey-Serres J., Freeling M. Hypoxic stress-induced changes in ribosomes of maize seedling roots. Plant Physiol. 1990 Nov;94(3):1237–1243. doi: 10.1104/pp.94.3.1237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dennis E. S., Gerlach W. L., Pryor A. J., Bennetzen J. L., Inglis A., Llewellyn D., Sachs M. M., Ferl R. J., Peacock W. J. Molecular analysis of the alcohol dehydrogenase (Adh1) gene of maize. Nucleic Acids Res. 1984 May 11;12(9):3983–4000. doi: 10.1093/nar/12.9.3983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dennis E. S., Sachs M. M., Gerlach W. L., Finnegan E. J., Peacock W. J. Molecular analysis of the alcohol dehydrogenase 2 (Adh2) gene of maize. Nucleic Acids Res. 1985 Feb 11;13(3):727–743. doi: 10.1093/nar/13.3.727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Feinberg A. P., Vogelstein B. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum. Anal Biochem. 1984 Feb;137(1):266–267. doi: 10.1016/0003-2697(84)90381-6. [DOI] [PubMed] [Google Scholar]
  5. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  6. Ferl R. J., Brennan M. D., Schwartz D. In vitro translation of maize ADH: evidence for the anaerobic induction of mRNA. Biochem Genet. 1980 Aug;18(7-8):681–691. doi: 10.1007/BF00484585. [DOI] [PubMed] [Google Scholar]
  7. Hole D. J., Cobb B. G., Hole P. S., Drew M. C. Enhancement of Anaerobic Respiration in Root Tips of Zea mays following Low-Oxygen (Hypoxic) Acclimation. Plant Physiol. 1992 May;99(1):213–218. doi: 10.1104/pp.99.1.213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hondred D., Hanson A. D. Hypoxically inducible barley lactate dehydrogenase: cDNA cloning and molecular analysis. Proc Natl Acad Sci U S A. 1990 Sep;87(18):7300–7304. doi: 10.1073/pnas.87.18.7300. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Johnson J., Cobb B. G., Drew M. C. Hypoxic Induction of Anoxia Tolerance in Root Tips of Zea mays. Plant Physiol. 1989 Nov;91(3):837–841. doi: 10.1104/pp.91.3.837. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kelley P. M. Maize pyruvate decarboxylase mRNA is induced anaerobically. Plant Mol Biol. 1989 Aug;13(2):213–222. doi: 10.1007/BF00016139. [DOI] [PubMed] [Google Scholar]
  11. Reuland-Bosma W., Pilot T., Henry P. P. Sulcusbloeding als indicator voor parodontale gezonheid bin kleuters tijdens een preventieprogramma. Ned Tijdschr Tandheelkd. 1982 Mar;89(3):118–122. [PubMed] [Google Scholar]
  12. Roberts J. K., Callis J., Jardetzky O., Walbot V., Freeling M. Cytoplasmic acidosis as a determinant of flooding intolerance in plants. Proc Natl Acad Sci U S A. 1984 Oct;81(19):6029–6033. doi: 10.1073/pnas.81.19.6029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rowland L. J., Strommer J. N. Anaerobic treatment of maize roots affects transcription of Adh1 and transcript stability. Mol Cell Biol. 1986 Oct;6(10):3368–3372. doi: 10.1128/mcb.6.10.3368. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sachs M. M., Freeling M., Okimoto R. The anaerobic proteins of maize. Cell. 1980 Jul;20(3):761–767. doi: 10.1016/0092-8674(80)90322-0. [DOI] [PubMed] [Google Scholar]
  15. Saglio P. H., Drew M. C., Pradet A. Metabolic Acclimation to Anoxia Induced by Low (2-4 kPa Partial Pressure) Oxygen Pretreatment (Hypoxia) in Root Tips of Zea mays. Plant Physiol. 1988 Jan;86(1):61–66. doi: 10.1104/pp.86.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Saglio P. H., Rancillac M., Bruzan F., Pradet A. Critical oxygen pressure for growth and respiration of excised and intact roots. Plant Physiol. 1984 Sep;76(1):151–154. doi: 10.1104/pp.76.1.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schneider-Gädicke A., Beer-Romero P., Brown L. G., Nussbaum R., Page D. C. ZFX has a gene structure similar to ZFY, the putative human sex determinant, and escapes X inactivation. Cell. 1989 Jun 30;57(7):1247–1258. doi: 10.1016/0092-8674(89)90061-5. [DOI] [PubMed] [Google Scholar]
  18. Springer B., Werr W., Starlinger P., Bennett D. C., Zokolica M., Freeling M. The Shrunken gene on chromosome 9 of Zea mays L is expressed in various plant tissues and encodes an anaerobic protein. Mol Gen Genet. 1986 Dec;205(3):461–468. doi: 10.1007/BF00338083. [DOI] [PubMed] [Google Scholar]
  19. Thomson C. J., Greenway H. Metabolic evidence for stelar anoxia in maize roots exposed to low o(2) concentrations. Plant Physiol. 1991 Aug;96(4):1294–1301. doi: 10.1104/pp.96.4.1294. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Walker J. C., Howard E. A., Dennis E. S., Peacock W. J. DNA sequences required for anaerobic expression of the maize alcohol dehydrogenase 1 gene. Proc Natl Acad Sci U S A. 1987 Oct;84(19):6624–6628. doi: 10.1073/pnas.84.19.6624. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Webster C., Gaut R. L., Browning K. S., Ravel J. M., Roberts J. K. Hypoxia enhances phosphorylation of eukaryotic initiation factor 4A in maize root tips. J Biol Chem. 1991 Dec 5;266(34):23341–23346. [PubMed] [Google Scholar]

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