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. 1993 Feb;101(2):435–440. doi: 10.1104/pp.101.2.435

Leaf Isoprene Emission Rate Is Dependent on Leaf Development and the Level of Isoprene Synthase.

J Kuzma 1, R Fall 1
PMCID: PMC160589  PMID: 12231698

Abstract

Isoprene (2-methyl-1,3-butadiene) is a major volatile hydrocarbon produced by many plant species. Here we report that in velvet bean (Mucuna sp.), isoprene emission is strongly dependent on leaf developmental state and that changes in extractable isoprene synthase activity parallel isoprene emission rates during leaf development. Both leaf emission and enzyme activity exhibit over 100-fold increases from leaf emergence to leaf age 14 d and exhibit similar patterns to 23 d. This suggests that the enzyme, isoprene synthase, is responsible for the in vivo production of isoprene and that the level of the enzyme regulates the pattern of isoprene emission in response to leaf development.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  3. Chameides W. L., Lindsay R. W., Richardson J., Kiang C. S. The role of biogenic hydrocarbons in urban photochemical smog: Atlanta as a case study. Science. 1988 Sep 16;241(4872):1473–1475. doi: 10.1126/science.3420404. [DOI] [PubMed] [Google Scholar]
  4. Grinspoon J., Bowman W. D., Fall R. Delayed Onset of Isoprene Emission in Developing Velvet Bean (Mucuna sp.) Leaves. Plant Physiol. 1991 Sep;97(1):170–174. doi: 10.1104/pp.97.1.170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Monson R. K., Fall R. Isoprene emission from aspen leaves : influence of environment and relation to photosynthesis and photorespiration. Plant Physiol. 1989 May;90(1):267–274. doi: 10.1104/pp.90.1.267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Monson R. K., Jaeger C. H., Adams W. W., Driggers E. M., Silver G. M., Fall R. Relationships among Isoprene Emission Rate, Photosynthesis, and Isoprene Synthase Activity as Influenced by Temperature. Plant Physiol. 1992 Mar;98(3):1175–1180. doi: 10.1104/pp.98.3.1175. [DOI] [PMC free article] [PubMed] [Google Scholar]

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