Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1993 Apr;101(4):1195–1199. doi: 10.1104/pp.101.4.1195

The Low CO2-Inducible 36-Kilodalton Protein Is Localized to the Chloroplast Envelope of Chlamydomonas reinhardtii.

Z Ramazanov 1, C B Mason 1, A M Geraghty 1, M H Spalding 1, J V Moroney 1
PMCID: PMC160639  PMID: 12231773

Abstract

The localization of the 36-kD polypeptide of Chlamydomonas reinhardtii induced by photoautotrophic growth on low CO2 concentrations (0.03% in air [v/v], low CO2-grown cells) has been investigated. This polypeptide was specifically localized to the chloroplast envelope membranes isolated from low CO2-grown cells and was not present in the chloroplast envelopes isolated from high (5% CO2 in air [v/v]) CO2-grown cells. The 36-kD protein does not show carbonic anhydrase activity and was not present on the plasma membranes isolated from low CO2-grown cells. This protein may, in part, account for the different inorganic carbon uptake characteristics observed in chloroplasts isolated from high and low CO2-grown cells of C. reinhardtii.

Full Text

The Full Text of this article is available as a PDF (1.6 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Badger M. R., Kaplan A., Berry J. A. Internal Inorganic Carbon Pool of Chlamydomonas reinhardtii: EVIDENCE FOR A CARBON DIOXIDE-CONCENTRATING MECHANISM. Plant Physiol. 1980 Sep;66(3):407–413. doi: 10.1104/pp.66.3.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  3. Cline K., Keegstra K. Galactosyltransferases involved in galactolipid biosynthesis are located in the outer membrane of pea chloroplast envelopes. Plant Physiol. 1983 Feb;71(2):366–372. doi: 10.1104/pp.71.2.366. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Coleman J. R., Grossman A. R. Biosynthesis of carbonic anhydrase in Chlamydomonas reinhardtii during adaptation to low CO(2). Proc Natl Acad Sci U S A. 1984 Oct;81(19):6049–6053. doi: 10.1073/pnas.81.19.6049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Geraghty A. M., Anderson J. C., Spalding M. H. A 36 Kilodalton Limiting-CO(2) Induced Polypeptide of Chlamydomonas Is Distinct from the 37 Kilodalton Periplasmic Carbonic Anhydrase. Plant Physiol. 1990 May;93(1):116–121. doi: 10.1104/pp.93.1.116. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mason C. B., Manuel L. J., Moroney J. V. A New Chloroplast Protein Is Induced by Growth on Low CO(2) in Chlamydomonas reinhardtii. Plant Physiol. 1990 Jun;93(2):833–836. doi: 10.1104/pp.93.2.833. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mason C. B., Matthews S., Bricker T. M., Moroney J. V. Simplified Procedure for the Isolation of Intact Chloroplasts from Chlamydomonas reinhardtii. Plant Physiol. 1991 Dec;97(4):1576–1580. doi: 10.1104/pp.97.4.1576. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Moroney J. V., Husic H. D., Tolbert N. E., Kitayama M., Manuel L. J., Togasaki R. K. Isolation and Characterization of a Mutant of Chlamydomonas reinhardtii Deficient in the CO(2) Concentrating Mechanism. Plant Physiol. 1989 Mar;89(3):897–903. doi: 10.1104/pp.89.3.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Moroney J. V., Kitayama M., Togasaki R. K., Tolbert N. E. Evidence for Inorganic Carbon Transport by Intact Chloroplasts of Chlamydomonas reinhardtii. Plant Physiol. 1987 Mar;83(3):460–463. doi: 10.1104/pp.83.3.460. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Spalding M. H., Jeffrey M. Membrane-Associated Polypeptides Induced in Chlamydomonas by Limiting CO(2) Concentrations. Plant Physiol. 1989 Jan;89(1):133–137. doi: 10.1104/pp.89.1.133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Thielmann J., Goyal A., Tolbert N. E. Two Polypeptides in the Inner Chloroplast Envelope of Dunaliella tertiolecta Induced by Low CO(2). Plant Physiol. 1992 Dec;100(4):2113–2115. doi: 10.1104/pp.100.4.2113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Thielmann J., Tolbert N. E., Goyal A., Senger H. Two Systems for Concentrating CO(2) and Bicarbonate during Photosynthesis by Scenedesmus. Plant Physiol. 1990 Mar;92(3):622–629. doi: 10.1104/pp.92.3.622. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES