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. 1993 Apr;101(4):1217–1221. doi: 10.1104/pp.101.4.1217

Elevated Levels of Both Sucrose-Phosphate Synthase and Sucrose Synthase in Vicia Guard Cells Indicate Cell-Specific Carbohydrate Interconversions.

DRC Hite 1, W H Outlaw Jr 1, M C Tarczynski 1
PMCID: PMC160642  PMID: 12231776

Abstract

A long series of reports correlate larger stomatal aperture size with elevated concentration of sucrose (Suc) in guard cells. To assess the role and autonomy of guard cells with respect to these changes, we have determined quantitatively the cellular distribution of the synthetic enzyme, Suc-phosphate synthase (SPS) and the degradative enzyme Suc synthase (SS) in Vicia leaflet. As expected for Suc-exporting cells, the photosynthetic parenchyma had a high SPS:SS ratio of approximately 45. Also as expected, in epidermal cells, which had only few and rudimentary plastids, the SPS:SS ratio was low (0.4). Of all cells and tissues measured, those that had the highest specific activity of SPS (about 4.8 [mu]mol mg-1 of protein h-1) were guard cells. Guard cells also had a very high relative specific activity of SS.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dali N., Michaud D., Yelle S. Evidence for the involvement of sucrose phosphate synthase in the pathway of sugar accumulation in sucrose-accumulating tomato fruits. Plant Physiol. 1992 Jun;99(2):434–438. doi: 10.1104/pp.99.2.434. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fischer R. A., Hsiao T. C. Stomatal Opening in Isolated Epidermal Strips of Vicia faba. II. Responses to KCl Concentration and the Role of Potassium Absorption. Plant Physiol. 1968 Dec;43(12):1953–1958. doi: 10.1104/pp.43.12.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fisher D. B. Sucrose Compartmentation in the Palisade Parenchyma of Vicia faba L. Plant Physiol. 1979 Sep;64(3):481–483. doi: 10.1104/pp.64.3.481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kato T., Berger S. J., Carter J. A., Lowry O. H. An enzymatic cycling method for nicotinamide-adenine dinucleotide with malic and alcohol dehydrogenases. Anal Biochem. 1973 May;53(1):86–97. doi: 10.1016/0003-2697(73)90409-0. [DOI] [PubMed] [Google Scholar]
  5. Outlaw W. H., Fisher D. B. Compartmentation in Vicia faba Leaves: I. Kinetics of C in the Tissues following Pulse Labeling. Plant Physiol. 1975 Apr;55(4):699–703. doi: 10.1104/pp.55.4.699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Outlaw W. H., Jr, Hite D. R., Fiore G. B. Removal of contaminating nucleoside diphosphates from commercial preparations of uridine diphosphoglucose. Anal Biochem. 1988 May 15;171(1):104–107. doi: 10.1016/0003-2697(88)90129-7. [DOI] [PubMed] [Google Scholar]
  7. Outlaw W. H., Springer S. A., Tarczynski M. C. Histochemical technique : a general method for quantitative enzyme assays of single cell ;extracts' with a time resolution of seconds and a reading precision of femtomoles. Plant Physiol. 1985 Mar;77(3):659–666. doi: 10.1104/pp.77.3.659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Poffenroth M., Green D. B., Tallman G. Sugar Concentrations in Guard Cells of Vicia faba Illuminated with Red or Blue Light : Analysis by High Performance Liquid Chromatography. Plant Physiol. 1992 Apr;98(4):1460–1471. doi: 10.1104/pp.98.4.1460. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sung S. J., Xu D. P., Black C. C. Identification of actively filling sucrose sinks. Plant Physiol. 1989 Apr;89(4):1117–1121. doi: 10.1104/pp.89.4.1117. [DOI] [PMC free article] [PubMed] [Google Scholar]

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