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. 1993 Apr;101(4):1385–1390. doi: 10.1104/pp.101.4.1385

Induction of Pyrophosphate-Dependent Phosphofructokinase in Watermelon (Citrullus lanatus) Cotyledons Coincides with Insufficient Cytosolic D-Fructose-1,6-Bisphosphate 1-Phosphohydrolase to Sustain Gluconeogenesis.

A M Botha 1, F C Botha 1
PMCID: PMC160664  PMID: 12231792

Abstract

During germination of Citrullus lanatus, pyrophosphate-dependent phosphofructokinase (PFP) activity is induced. The peak of PFP activity coincides with the maximum gluconeogenic flux and high fructose-2,6-bisphosphate (Fru-2,6-P2) concentrations. Determination of cytosolic fructose-1,6 bisphosphatase (FBPase) activity in crude extracts is unreliable because of the high PFP activity. The FBPase activity, after correction for the contaminating PFP, is only one-third of the PFP activity. Purified cytosolic FBPase is inhibited by Fru-2,6-P2. The low cytosolic FBPase activity and high Fru-2,6-P2 most probably result in inadequate in vivo activity to catalyze the observed gluconeogenic flux. The total PFP activity is sufficient to catalyze the required carbon flux.

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Selected References

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  1. Ap Rees T., Thomas S. M., Fuller W. A., Chapman B. Location of gluconeogenesis from phosphoenolpyruvate in cotyledons of Cucurbita pepo. Biochim Biophys Acta. 1975 Mar 14;385(1):145–156. doi: 10.1016/0304-4165(75)90082-3. [DOI] [PubMed] [Google Scholar]
  2. Botha A. M., Botha F. C. Control of Pyrophosphated-Fructose-6-Phosphate 1-Phosphotransferase Activity in the Cotyledons of Citrullus lanatus. Plant Physiol. 1990 Jun;93(2):683–688. doi: 10.1104/pp.93.2.683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Botha A. M., Botha F. C. Pyrophosphate Dependent Phosphofructokinase of Citrullus lanatus: Molecular Forms and Expression of Subunits. Plant Physiol. 1991 Aug;96(4):1185–1192. doi: 10.1104/pp.96.4.1185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  5. Cséke C., Weeden N. F., Buchanan B. B., Uyeda K. A special fructose bisphosphate functions as a cytoplasmic regulatory metabolite in green leaves. Proc Natl Acad Sci U S A. 1982 Jul;79(14):4322–4326. doi: 10.1073/pnas.79.14.4322. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gunasekaran M., Hughes W. T. Gas-liquid chromatography: a rapid method for identification of different species of Candida. Mycologia. 1980 May-Jun;72(3):505–511. [PubMed] [Google Scholar]
  7. Kombrink E., Kruger N. J., Beevers H. Kinetic properties of pyrophosphate:fructose-6-phosphate phosphotransferase from germinating castor bean endosperm. Plant Physiol. 1984 Feb;74(2):395–401. doi: 10.1104/pp.74.2.395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kruger N. J., Beevers H. Effect of fructose 2,6-bisphosphate on the kinetic properties of cytoplasmic fructose 1,6-bisphosphatase from germinating castor bean endosperm. Plant Physiol. 1984 Sep;76(1):49–54. doi: 10.1104/pp.76.1.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kruger N. J., Beevers H. Synthesis and degradation of fructose 2,6-bisphosphate in endosperm of castor bean seedlings. Plant Physiol. 1985 Feb;77(2):358–364. doi: 10.1104/pp.77.2.358. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Leegood R. C., ap Rees T. Identification of the regulatory steps in gluconeogenesis in cotyledons of Cucurbita pepo. Biochim Biophys Acta. 1978 Aug 3;542(1):1–11. doi: 10.1016/0304-4165(78)90226-x. [DOI] [PubMed] [Google Scholar]
  11. Muto S., Beevers H. Lipase Activities in Castor Bean Endosperm during Germination. Plant Physiol. 1974 Jul;54(1):23–28. doi: 10.1104/pp.54.1.23. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sabularse D. C., Anderson R. L. D-Fructose 2,6-bisphosphate: a naturally occurring activator for inorganic pyrophosphate:D-fructose-6-phosphate 1-phosphotransferase in plants. Biochem Biophys Res Commun. 1981 Dec 15;103(3):848–855. doi: 10.1016/0006-291x(81)90888-3. [DOI] [PubMed] [Google Scholar]
  13. Sabularse D. C., Anderson R. L. Inorganic pyrophosphate: D-fructose-6-phosphate 1-phosphotransferase in mung beans and its activation by D-fructose 1,6-bisphosphate and D-glucose 1, 6-bisphosphate. Biochem Biophys Res Commun. 1981 Jun;100(4):1423–1429. doi: 10.1016/0006-291x(81)90677-x. [DOI] [PubMed] [Google Scholar]
  14. Stitt M., Gerhardt R., Kürzel B., Heldt H. W. A role for fructose 2,6-bisphosphate in the regulation of sucrose synthesis in spinach leaves. Plant Physiol. 1983 Aug;72(4):1139–1141. doi: 10.1104/pp.72.4.1139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Stitt M. Product inhibition of potato tuber pyrophosphate:fructose-6-phosphate phosphotransferase by phosphate and pyrophosphate. Plant Physiol. 1989 Feb;89(2):628–633. doi: 10.1104/pp.89.2.628. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Van Schaftingen E., Lederer B., Bartrons R., Hers H. G. A kinetic study of pyrophosphate: fructose-6-phosphate phosphotransferase from potato tubers. Application to a microassay of fructose 2,6-bisphosphate. Eur J Biochem. 1982 Dec;129(1):191–195. doi: 10.1111/j.1432-1033.1982.tb07039.x. [DOI] [PubMed] [Google Scholar]
  17. ap Rees T., Green J. H., Wilson P. M. Pyrophosphate:fructose 6-phosphate 1-phosphotransferase and glycolysis in non-photosynthetic tissues of higher plants. Biochem J. 1985 Apr 1;227(1):299–304. doi: 10.1042/bj2270299. [DOI] [PMC free article] [PubMed] [Google Scholar]

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