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. 1994 Mar;104(3):1027–1032. doi: 10.1104/pp.104.3.1027

Light-Stimulated Cotyledon Expansion in Arabidopsis Seedlings (The Role of Phytochrome B).

M M Neff 1, E Van Volkenburgh 1
PMCID: PMC160701  PMID: 12232145

Abstract

Leaf and cotyledon expansion in dicotyledonous plants is a light-dependent developmental process. The unique role of phytochrome B has been tested by investigating expansion of cotyledons in wild-type and phytochrome-deficient mutants of Arabidopsis thaliana (L.) Heynh. A relatively rapid method for measuring cotyledon area was developed to quantify growth in large populations (average n [greater than or equal to] 100) of wild-type or mutant seedlings under different light and chemical treatments. Three-day-old wild-type (La-er) Arabidopsis seedlings, grown in saturating, low-fluence red light (2-4 [mu]mol m-2 s-1), showed a >250% increase in cotyledon area after 48 h of bright-red light when compared with the phytochrome mutants hy1, hy2, and hy3. An increase in epidermal cell area was observed in wild-type cotyledons but not in hy3, indicating that light-stimulated growth is due in part to cell expansion. The mutant phenotype was rescued by feeding the chromophore precursor biliverdin to the chromophore biosynthesis mutants hy1 and hy6. This treatment did not rescue the hy3 mutant. Since the hy3 lesion is specific to phytochrome B, we conclude that this pigment is involved in the enhancement of cotyledon cell expansion in bright-red light.

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Selected References

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  1. Blum D. E., Elzenga J. T., Linnemeyer P. A., Van Volkenburgh E. Stimulation of growth and ion uptake in bean leaves by red and blue light. Plant Physiol. 1992 Dec;100(4):1968–1975. doi: 10.1104/pp.100.4.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chory J., Nagpal P., Peto C. A. Phenotypic and Genetic Analysis of det2, a New Mutant That Affects Light-Regulated Seedling Development in Arabidopsis. Plant Cell. 1991 May;3(5):445–459. doi: 10.1105/tpc.3.5.445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chory J., Peto C. A., Ashbaugh M., Saganich R., Pratt L., Ausubel F. Different Roles for Phytochrome in Etiolated and Green Plants Deduced from Characterization of Arabidopsis thaliana Mutants. Plant Cell. 1989 Sep;1(9):867–880. doi: 10.1105/tpc.1.9.867. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cosgrove D. Biophysical control of plant cell growth. Annu Rev Plant Physiol. 1986;37:377–405. doi: 10.1146/annurev.pp.37.060186.002113. [DOI] [PubMed] [Google Scholar]
  5. Deng X. W., Caspar T., Quail P. H. cop1: a regulatory locus involved in light-controlled development and gene expression in Arabidopsis. Genes Dev. 1991 Jul;5(7):1172–1182. doi: 10.1101/gad.5.7.1172. [DOI] [PubMed] [Google Scholar]
  6. Devlin P. F., Rood S. B., Somers D. E., Quail P. H., Whitelam G. C. Photophysiology of the Elongated Internode (ein) Mutant of Brassica rapa: ein Mutant Lacks a Detectable Phytochrome B-Like Polypeptide. Plant Physiol. 1992 Nov;100(3):1442–1447. doi: 10.1104/pp.100.3.1442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fallon K. M., Shacklock P. S., Trewavas A. J. Detection in Vivo of Very Rapid Red Light-Induced Calcium-Sensitive Protein Phosphorylation in Etiolated Wheat (Triticum aestivum) Leaf Protoplasts. Plant Physiol. 1993 Mar;101(3):1039–1045. doi: 10.1104/pp.101.3.1039. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hou Y., Von Arnim A. G., Deng X. W. A New Class of Arabidopsis Constitutive Photomorphogenic Genes Involved in Regulating Cotyledon Development. Plant Cell. 1993 Mar;5(3):329–339. doi: 10.1105/tpc.5.3.329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Khurana J. P., Poff K. L. Mutants of Arabidopsis thaliana with altered phototropism. Planta. 1989;178:400–406. [PubMed] [Google Scholar]
  10. Nagatani A., Reed J. W., Chory J. Isolation and Initial Characterization of Arabidopsis Mutants That Are Deficient in Phytochrome A. Plant Physiol. 1993 May;102(1):269–277. doi: 10.1104/pp.102.1.269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Parks B. M., Quail P. H. Phytochrome-Deficient hy1 and hy2 Long Hypocotyl Mutants of Arabidopsis Are Defective in Phytochrome Chromophore Biosynthesis. Plant Cell. 1991 Nov;3(11):1177–1186. doi: 10.1105/tpc.3.11.1177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Parks B. M., Quail P. H. hy8, a new class of arabidopsis long hypocotyl mutants deficient in functional phytochrome A. Plant Cell. 1993 Jan;5(1):39–48. doi: 10.1105/tpc.5.1.39. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Reed J. W., Nagpal P., Poole D. S., Furuya M., Chory J. Mutations in the gene for the red/far-red light receptor phytochrome B alter cell elongation and physiological responses throughout Arabidopsis development. Plant Cell. 1993 Feb;5(2):147–157. doi: 10.1105/tpc.5.2.147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Somers D. E., Sharrock R. A., Tepperman J. M., Quail P. H. The hy3 Long Hypocotyl Mutant of Arabidopsis Is Deficient in Phytochrome B. Plant Cell. 1991 Dec;3(12):1263–1274. doi: 10.1105/tpc.3.12.1263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Valvekens D., Van Montagu M., Van Lijsebettens M. Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection. Proc Natl Acad Sci U S A. 1988 Aug;85(15):5536–5540. doi: 10.1073/pnas.85.15.5536. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wei N., Deng X. W. COP9: a new genetic locus involved in light-regulated development and gene expression in arabidopsis. Plant Cell. 1992 Dec;4(12):1507–1518. doi: 10.1105/tpc.4.12.1507. [DOI] [PMC free article] [PubMed] [Google Scholar]

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