Abstract
Photoacclimation was studied in tobacco leaves (Nicotiana tabacum cv Xanthi) infected with two strains of tobacco mosaic virus (TMV) and grown under different light and nitrogen nutrition regimes. Photosynthetic acclimation measured by the quantum yield and the maximum rate in saturating light of CO2-saturated photosynthesis was impaired to a greater extent in tobacco leaves infected with TMV strain PV230 than in those infected with TMV strain PV42. Infection with TMV strain PV230 severely impaired photosynthetic acclimation at high light/low nitrogen and during transfer from low to high light. Expanding leaves showing chlorotic-mosaic symptoms had greatly reduced capacity to acclimate to high light compared with controls and with developed leaves without visible symptoms. We conclude that the failure of expanding leaves to acclimate was largely due to the destruction of chloroplasts in yellow areas of the tissue, accompanied by severe reduction in ribulose-1,5-bisphosphate carboxylase/oxygenase levels, and corresponding reduction in photosynthesis on a leaf-area basis. When corrected for areas of healthy green tissue, photoacclimation of infected leaves was the same as that of controls. Visible symptom development was greatest in high light/low nitrogen treatments. In developed leaves without visible symptoms, virus accumulation, which was as extensive as in expanding leaves, accelerated senescence and impaired photoacclimation during transfer from low light to high light. Generally, infection with TMV strain PV42 did not impair photosynthetic acclimation and even enhanced it in some treatments, even though virus accumulated to the same concentration as in PV230-infected leaves. These data show that TMV does not simply impair photoacclimation in tobacco by competing with chloroplasts for leaf nitrogen reserves. Rather, specific properties of severe strains, such as PV230, which lead to visible symptom development and patchy loss of photosynthetic activity in expanding leaves as well as general acceleration of chloroplast senescence in developed leaves, contribute to impaired photoacclimation, which is generally exacerbated by low nitrogen nutrition.
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Selected References
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- Balachandran S., Osmond C. B., Daley P. F. Diagnosis of the Earliest Strain-Specific Interactions between Tobacco Mosaic Virus and Chloroplasts of Tobacco Leaves in Vivo by Means of Chlorophyll Fluorescence Imaging. Plant Physiol. 1994 Mar;104(3):1059–1065. doi: 10.1104/pp.104.3.1059. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Balachandran S., Osmond C. B. Susceptibility of Tobacco Leaves to Photoinhibition following Infection with Two Strains of Tobacco Mosaic Virus under Different Light and Nitrogen Nutrition Regimes. Plant Physiol. 1994 Mar;104(3):1051–1057. doi: 10.1104/pp.104.3.1051. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CHOPPIN P. W. MULTIPLICATION OF A MYXOVIRUS (SV5) WITH MINIMAL CYTOPATHIC EFFECTS AND WITHOUT INTERFERENCE. Virology. 1964 Jun;23:224–233. doi: 10.1016/0042-6822(64)90286-7. [DOI] [PubMed] [Google Scholar]
- Esau K., Cronshaw J. Relation of tobacco mosaic virus to the host cells. J Cell Biol. 1967 Jun;33(3):665–678. doi: 10.1083/jcb.33.3.665. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hodgson R. A., Beachy R. N., Pakrasi H. B. Selective inhibition of photosystem II in spinach by tobacco mosaic virus: an effect of the viral coat protein. FEBS Lett. 1989 Mar 13;245(1-2):267–270. doi: 10.1016/0014-5793(89)80234-0. [DOI] [PubMed] [Google Scholar]
- Makino A., Osmond B. Effects of Nitrogen Nutrition on Nitrogen Partitioning between Chloroplasts and Mitochondria in Pea and Wheat. Plant Physiol. 1991 Jun;96(2):355–362. doi: 10.1104/pp.96.2.355. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reinero A., Beachy R. N. Reduced Photosystem II Activity and Accumulation of Viral Coat Protein in Chloroplasts of Leaves Infected with Tobacco Mosaic Virus. Plant Physiol. 1989 Jan;89(1):111–116. doi: 10.1104/pp.89.1.111. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ZAITLIN M., JAGENDORF A. T. Photosynthetic phosphorylation and Hill reaction activities of chloroplasts isolated from plants infected with tobacco mosaic virus. Virology. 1960 Nov;12:477–486. doi: 10.1016/0042-6822(60)90168-9. [DOI] [PubMed] [Google Scholar]
- van Loon L. C. Disease induction by plant viruses. Adv Virus Res. 1987;33:205–255. doi: 10.1016/s0065-3527(08)60319-x. [DOI] [PubMed] [Google Scholar]