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. 1994 Jul;105(3):881–889. doi: 10.1104/pp.105.3.881

Flavonoids can protect maize DNA from the induction of ultraviolet radiation damage.

A E Stapleton 1, V Walbot 1
PMCID: PMC160736  PMID: 8058838

Abstract

Diverse flavonoid compounds are widely distributed in angiosperm families. Flavonoids absorb radiation in the ultraviolet (UV) region of the spectrum, and it has been proposed that these compounds function as UV filters. We demonstrate that the DNA in Zea mays plants that contain flavonoids (primarily anthocyanins) is protected from the induction of damage caused by UV radiation relative to the DNA in plants that are genetically deficient in these compounds. DNA damage was measured with a sensitive and simple assay using individual monoclonal antibodies, one specific for cyclobutane pyrimidine dimer damage and the other specific for pyrimidine(6,4)pyrimidone damage.

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Selected References

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  1. Cadet J., Anselmino C., Douki T., Voituriez L. Photochemistry of nucleic acids in cells. J Photochem Photobiol B. 1992 Sep 15;15(4):277–298. doi: 10.1016/1011-1344(92)85135-h. [DOI] [PubMed] [Google Scholar]
  2. Freeman S. E., Blackett A. D., Monteleone D. C., Setlow R. B., Sutherland B. M., Sutherland J. C. Quantitation of radiation-, chemical-, or enzyme-induced single strand breaks in nonradioactive DNA by alkaline gel electrophoresis: application to pyrimidine dimers. Anal Biochem. 1986 Oct;158(1):119–129. doi: 10.1016/0003-2697(86)90599-3. [DOI] [PubMed] [Google Scholar]
  3. Li J., Ou-Lee T. M., Raba R., Amundson R. G., Last R. L. Arabidopsis Flavonoid Mutants Are Hypersensitive to UV-B Irradiation. Plant Cell. 1993 Feb;5(2):171–179. doi: 10.1105/tpc.5.2.171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Middleton E. M., Teramura A. H. The Role of Flavonol Glycosides and Carotenoids in Protecting Soybean from Ultraviolet-B Damage. Plant Physiol. 1993 Nov;103(3):741–752. doi: 10.1104/pp.103.3.741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Mirecki R. M., Teramura A. H. Effects of Ultraviolet-B Irradiance on Soybean : V. The Dependence of Plant Sensitivity on the Photosynthetic Photon Flux Density during and after Leaf Expansion. Plant Physiol. 1984 Mar;74(3):475–480. doi: 10.1104/pp.74.3.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mitchell D. L., Jen J., Cleaver J. E. Sequence specificity of cyclobutane pyrimidine dimers in DNA treated with solar (ultraviolet B) radiation. Nucleic Acids Res. 1992 Jan 25;20(2):225–229. doi: 10.1093/nar/20.2.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mitchell D. L., Nairn R. S. The biology of the (6-4) photoproduct. Photochem Photobiol. 1989 Jun;49(6):805–819. doi: 10.1111/j.1751-1097.1989.tb05578.x. [DOI] [PubMed] [Google Scholar]
  8. Mori T., Nakane M., Hattori T., Matsunaga T., Ihara M., Nikaido O. Simultaneous establishment of monoclonal antibodies specific for either cyclobutane pyrimidine dimer or (6-4)photoproduct from the same mouse immunized with ultraviolet-irradiated DNA. Photochem Photobiol. 1991 Aug;54(2):225–232. doi: 10.1111/j.1751-1097.1991.tb02010.x. [DOI] [PubMed] [Google Scholar]
  9. Pang Q., Hays J. B. UV-B-Inducible and Temperature-Sensitive Photoreactivation of Cyclobutane Pyrimidine Dimers in Arabidopsis thaliana. Plant Physiol. 1991 Feb;95(2):536–543. doi: 10.1104/pp.95.2.536. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Parrish J. A., Jaenicke K. F., Anderson R. R. Erythema and melanogenesis action spectra of normal human skin. Photochem Photobiol. 1982 Aug;36(2):187–191. doi: 10.1111/j.1751-1097.1982.tb04362.x. [DOI] [PubMed] [Google Scholar]
  11. Ryder T. B., Hedrick S. A., Bell J. N., Liang X. W., Clouse S. D., Lamb C. J. Organization and differential activation of a gene family encoding the plant defense enzyme chalcone synthase in Phaseolus vulgaris. Mol Gen Genet. 1987 Dec;210(2):219–233. doi: 10.1007/BF00325687. [DOI] [PubMed] [Google Scholar]
  12. Stapleton A. E. Ultraviolet Radiation and Plants: Burning Questions. Plant Cell. 1992 Nov;4(11):1353–1358. doi: 10.1105/tpc.4.11.1353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Walbot V., Warren C. Regulation of Mu element copy number in maize lines with an active or inactive Mutator transposable element system. Mol Gen Genet. 1988 Jan;211(1):27–34. doi: 10.1007/BF00338389. [DOI] [PubMed] [Google Scholar]

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