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. 1995 May;7(5):649–658. doi: 10.1105/tpc.7.5.649

Inactivation of a Synechocystis sp strain PCC 6803 gene with homology to conserved chloroplast open reading frame 184 increases the photosystem II-to-photosystem I ratio.

A Wilde 1, H Härtel 1, T Hübschmann 1, P Hoffmann 1, S V Shestakov 1, T Börner 1
PMCID: PMC160811  PMID: 7780311

Abstract

A gene of the unicellular cyanobacterium Synechocystis sp strain PCC 6803 that is homologous to the conserved chloroplast open reading frame orf184 has been cloned and sequenced. The nucleotide sequence of the gene predicts a protein of 184 amino acids with a calculated molecular mass of 21.5 kD and two membrane-spanning regions. Amino acid sequence analysis showed 46 to 37% homology of the cyanobacterial orf184 with tobacco orf184, rice orf185, liverwort orf184, and Euglena gracilis orf206 sequences. Two orf184-specific mutants of Synechocystis sp PCC 6803 were constructed by insertion mutagenesis. Cells of mutants showed growth characteristics similar to those of the wild type. Their pigment composition was distinctly different from the wild type, as indicated by an increase in the phycocyanin-to-chlorophyll ratio. In addition, mutants also had a two- to threefold increase in photosynthetic electron transfer rates as well as in photosystem II-to-photosystem I ratio-a phenomenon hitherto not reported for mutants with altered photosynthetic characteristics. The observed alterations in the orf184-specific mutants provide strong evidence for a functional role of the orf184 gene product in photosynthetic processes.

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Selected References

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  1. Anderson S. L., McIntosh L. Partial conservation of the 5' ndhE-psaC-ndhD 3' gene arrangement of chloroplasts in the cyanobacterium Synechocystis sp. PCC 6803: implications for NDH-D function in cyanobacteria and chloroplasts. Plant Mol Biol. 1991 Apr;16(4):487–499. doi: 10.1007/BF00023416. [DOI] [PubMed] [Google Scholar]
  2. Chitnis P. R., Purvis D., Nelson N. Molecular cloning and targeted mutagenesis of the gene psaF encoding subunit III of photosystem I from the cyanobacterium Synechocystis sp. PCC 6803. J Biol Chem. 1991 Oct 25;266(30):20146–20151. [PubMed] [Google Scholar]
  3. Chitnis V. P., Xu Q., Yu L., Golbeck J. H., Nakamoto H., Xie D. L., Chitnis P. R. Targeted inactivation of the gene psaL encoding a subunit of photosystem I of the cyanobacterium Synechocystis sp. PCC 6803. J Biol Chem. 1993 Jun 5;268(16):11678–11684. [PubMed] [Google Scholar]
  4. Chow W. S., Melis A., Anderson J. M. Adjustments of photosystem stoichiometry in chloroplasts improve the quantum efficiency of photosynthesis. Proc Natl Acad Sci U S A. 1990 Oct;87(19):7502–7506. doi: 10.1073/pnas.87.19.7502. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Clarke A. K., Hurry V. M., Gustafsson P., Oquist G. Two functionally distinct forms of the photosystem II reaction-center protein D1 in the cyanobacterium Synechococcus sp. PCC 7942. Proc Natl Acad Sci U S A. 1993 Dec 15;90(24):11985–11989. doi: 10.1073/pnas.90.24.11985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hiratsuka J., Shimada H., Whittier R., Ishibashi T., Sakamoto M., Mori M., Kondo C., Honji Y., Sun C. R., Meng B. Y. The complete sequence of the rice (Oryza sativa) chloroplast genome: intermolecular recombination between distinct tRNA genes accounts for a major plastid DNA inversion during the evolution of the cereals. Mol Gen Genet. 1989 Jun;217(2-3):185–194. doi: 10.1007/BF02464880. [DOI] [PubMed] [Google Scholar]
  7. Logemann J., Schell J., Willmitzer L. Improved method for the isolation of RNA from plant tissues. Anal Biochem. 1987 May 15;163(1):16–20. doi: 10.1016/0003-2697(87)90086-8. [DOI] [PubMed] [Google Scholar]
  8. Ogura Y., Yoshida T., Nakamura Y., Takemura M., Oda K., Ohyama K. Gene encoding a putative zinc finger protein in Synechocystis PCC6803. Agric Biol Chem. 1991 Sep;55(9):2259–2264. [PubMed] [Google Scholar]
  9. Reilly P., Hulmes J. D., Pan Y. C., Nelson N. Molecular cloning and sequencing of the psaD gene encoding subunit II of photosystem I from the cyanobacterium, Synechocystis sp. PCC 6803. J Biol Chem. 1988 Nov 25;263(33):17658–17662. [PubMed] [Google Scholar]
  10. Rögner M., Nixon P. J., Diner B. A. Purification and characterization of photosystem I and photosystem II core complexes from wild-type and phycocyanin-deficient strains of the cyanobacterium Synechocystis PCC 6803. J Biol Chem. 1990 Apr 15;265(11):6189–6196. [PubMed] [Google Scholar]
  11. Samson G., Herbert S. K., Fork D. C., Laudenbach D. E. Acclimation of the Photosynthetic Apparatus to Growth Irradiance in a Mutant Strain of Synechococcus Lacking Iron Superoxide Dismutase. Plant Physiol. 1994 May;105(1):287–294. doi: 10.1104/pp.105.1.287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shen G., Vermaas W. F. Chlorophyll in a Synechocystis sp. PCC 6803 mutant without photosystem I and photosystem II core complexes. Evidence for peripheral antenna chlorophylls in cyanobacteria. J Biol Chem. 1994 May 13;269(19):13904–13910. [PubMed] [Google Scholar]
  13. Shimada H., Sugiura M. Fine structural features of the chloroplast genome: comparison of the sequenced chloroplast genomes. Nucleic Acids Res. 1991 Mar 11;19(5):983–995. doi: 10.1093/nar/19.5.983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Smart L. B., Bowlby N. R., Anderson S. L., Sithole I., McIntosh L. Genetic Manipulation of the Cyanobacterium Synechocystis sp. PCC 6803 (Development of Strains Lacking Photosystem I for the Analysis of Mutations in Photosystem II). Plant Physiol. 1994 Feb;104(2):349–354. doi: 10.1104/pp.104.2.349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Staub J. M., Maliga P. Long regions of homologous DNA are incorporated into the tobacco plastid genome by transformation. Plant Cell. 1992 Jan;4(1):39–45. doi: 10.1105/tpc.4.1.39. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Vermaas W., Charité J., Shen G. Z. Glu-69 of the D2 protein in photosystem II is a potential ligand to Mn involved in photosynthetic oxygen evolution. Biochemistry. 1990 Jun 5;29(22):5325–5332. doi: 10.1021/bi00474a017. [DOI] [PubMed] [Google Scholar]
  17. Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. doi: 10.1016/0378-1119(82)90015-4. [DOI] [PubMed] [Google Scholar]
  18. Williams R. C., Glazer A. N., Lundell D. J. Cyanobacterial photosystem I: Morphology and aggregation behavior. Proc Natl Acad Sci U S A. 1983 Oct;80(19):5923–5926. doi: 10.1073/pnas.80.19.5923. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wolfe K. H., Morden C. W., Ems S. C., Palmer J. D. Rapid evolution of the plastid translational apparatus in a nonphotosynthetic plant: loss or accelerated sequence evolution of tRNA and ribosomal protein genes. J Mol Evol. 1992 Oct;35(4):304–317. doi: 10.1007/BF00161168. [DOI] [PubMed] [Google Scholar]
  20. Wolfe K. H., Morden C. W., Palmer J. D. Function and evolution of a minimal plastid genome from a nonphotosynthetic parasitic plant. Proc Natl Acad Sci U S A. 1992 Nov 15;89(22):10648–10652. doi: 10.1073/pnas.89.22.10648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wolfe K. H. Similarity between putative ATP-binding sites in land plant plastid ORF2280 proteins and the FtsH/CDC48 family of ATPases. Curr Genet. 1994 Apr;25(4):379–383. doi: 10.1007/BF00351493. [DOI] [PubMed] [Google Scholar]

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