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. 1995 Jun;7(6):667–676. doi: 10.1105/tpc.7.6.667

Expression and Regulation of aERD2, a Gene Encoding the KDEL Receptor Homolog in Plants, and Other Genes Encoding Proteins Involved in ER-Golgi Vesicular Trafficking.

M Bar-Peled 1, AdS Conceicao 1, L Frigerio 1, N V Raikhel 1
PMCID: PMC160814  PMID: 12242382

Abstract

aERD2 and aSAR1 of Arabidopsis are functional homologs of yeast genes encoding proteins essential for endoplasmic reticulum (ER)-to-Golgi transport. The regulation of these secretory pathway genes in yeast, mammals, and plants is not known. High levels of expression of aERD2 and aSAR1 were observed in roots, flowers, and inflorescence stems, with the highest levels being detected in roots. The aSAR1 transcript levels were highest in young leaves and declined during leaf maturation. Low levels of aERD2 were detected in both young and fully mature leaves when compared with roots. In situ hybridization showed that trichomes accumulate more aERD2 transcript as the leaf expands, whereas aSAR1 is expressed equally in all leaf cell types. Treating plants with tunicamycin, a drug that blocks N-glycosylation in the ER, or with cold shock, known to block secretory protein transport, led to a marked accumulation of aERD2 and aSAR1 transcripts. The Arabidopsis ARF gene, which encodes a GTPase probably involved in Golgi vesicle traffic, was not affected by these treatments. This study is an essential first step toward understanding the regulation of genes that encode proteins involved in vesicular trafficking.

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Selected References

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  1. D'Amico L., Valsasina B., Daminati M. G., Fabbrini M. S., Nitti G., Bollini R., Ceriotti A., Vitale A. Bean homologs of the mammalian glucose-regulated proteins: induction by tunicamycin and interaction with newly synthesized seed storage proteins in the endoplasmic reticulum. Plant J. 1992 Jul;2(4):443–455. doi: 10.1111/j.1365-313x.1992.00443.x. [DOI] [PubMed] [Google Scholar]
  2. Davies C. Cloning and characterization of a tomato GTPase-like gene related to yeast and Arabidopsis genes involved in vesicular transport. Plant Mol Biol. 1994 Feb;24(3):525–531. doi: 10.1007/BF00024120. [DOI] [PubMed] [Google Scholar]
  3. De Block M., Debrouwer D. RNA-RNA in situ hybridization using digoxigenin-labeled probes: the use of high-molecular-weight polyvinyl alcohol in the alkaline phosphatase indoxyl-nitroblue tetrazolium reaction. Anal Biochem. 1993 Nov 15;215(1):86–89. doi: 10.1006/abio.1993.1558. [DOI] [PubMed] [Google Scholar]
  4. Dean N., Pelham H. R. Recycling of proteins from the Golgi compartment to the ER in yeast. J Cell Biol. 1990 Aug;111(2):369–377. doi: 10.1083/jcb.111.2.369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Denecke J., De Rycke R., Botterman J. Plant and mammalian sorting signals for protein retention in the endoplasmic reticulum contain a conserved epitope. EMBO J. 1992 Jun;11(6):2345–2355. doi: 10.1002/j.1460-2075.1992.tb05294.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fontes E. B., Shank B. B., Wrobel R. L., Moose S. P., OBrian G. R., Wurtzel E. T., Boston R. S. Characterization of an immunoglobulin binding protein homolog in the maize floury-2 endosperm mutant. Plant Cell. 1991 May;3(5):483–496. doi: 10.1105/tpc.3.5.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hall T. C., Ma Y., Buchbinder B. U., Pyne J. W., Sun S. M., Bliss F. A. Messenger RNA for G1 protein of French bean seeds: Cell-free translation and product characterization. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3196–3200. doi: 10.1073/pnas.75.7.3196. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kaiser C. A., Schekman R. Distinct sets of SEC genes govern transport vesicle formation and fusion early in the secretory pathway. Cell. 1990 May 18;61(4):723–733. doi: 10.1016/0092-8674(90)90483-u. [DOI] [PubMed] [Google Scholar]
  9. Larkin J. C., Oppenheimer D. G., Lloyd A. M., Paparozzi E. T., Marks M. D. Roles of the GLABROUS1 and TRANSPARENT TESTA GLABRA Genes in Arabidopsis Trichome Development. Plant Cell. 1994 Aug;6(8):1065–1076. doi: 10.1105/tpc.6.8.1065. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lee H. I., Gal S., Newman T. C., Raikhel N. V. The Arabidopsis endoplasmic reticulum retention receptor functions in yeast. Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):11433–11437. doi: 10.1073/pnas.90.23.11433. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lewis M. J., Pelham H. R. A human homologue of the yeast HDEL receptor. Nature. 1990 Nov 8;348(6297):162–163. doi: 10.1038/348162a0. [DOI] [PubMed] [Google Scholar]
  12. Lewis M. J., Pelham H. R. Ligand-induced redistribution of a human KDEL receptor from the Golgi complex to the endoplasmic reticulum. Cell. 1992 Jan 24;68(2):353–364. doi: 10.1016/0092-8674(92)90476-s. [DOI] [PubMed] [Google Scholar]
  13. Lloyd A. M., Walbot V., Davis R. W. Arabidopsis and Nicotiana anthocyanin production activated by maize regulators R and C1. Science. 1992 Dec 11;258(5089):1773–1775. doi: 10.1126/science.1465611. [DOI] [PubMed] [Google Scholar]
  14. Munro S., Pelham H. R. A C-terminal signal prevents secretion of luminal ER proteins. Cell. 1987 Mar 13;48(5):899–907. doi: 10.1016/0092-8674(87)90086-9. [DOI] [PubMed] [Google Scholar]
  15. Nakańo A., Muramatsu M. A novel GTP-binding protein, Sar1p, is involved in transport from the endoplasmic reticulum to the Golgi apparatus. J Cell Biol. 1989 Dec;109(6 Pt 1):2677–2691. doi: 10.1083/jcb.109.6.2677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Noiva R., Lennarz W. J. Protein disulfide isomerase. A multifunctional protein resident in the lumen of the endoplasmic reticulum. J Biol Chem. 1992 Feb 25;267(6):3553–3556. [PubMed] [Google Scholar]
  17. Palade G. Intracellular aspects of the process of protein synthesis. Science. 1975 Aug 1;189(4200):347–358. doi: 10.1126/science.1096303. [DOI] [PubMed] [Google Scholar]
  18. Regad F., Bardet C., Tremousaygue D., Moisan A., Lescure B., Axelos M. cDNA cloning and expression of an Arabidopsis GTP-binding protein of the ARF family. FEBS Lett. 1993 Jan 25;316(2):133–136. doi: 10.1016/0014-5793(93)81201-a. [DOI] [PubMed] [Google Scholar]
  19. Rothman J. E. Mechanisms of intracellular protein transport. Nature. 1994 Nov 3;372(6501):55–63. doi: 10.1038/372055a0. [DOI] [PubMed] [Google Scholar]
  20. Saraste J., Kuismanen E. Pre- and post-Golgi vacuoles operate in the transport of Semliki Forest virus membrane glycoproteins to the cell surface. Cell. 1984 Sep;38(2):535–549. doi: 10.1016/0092-8674(84)90508-7. [DOI] [PubMed] [Google Scholar]
  21. Semenza J. C., Hardwick K. G., Dean N., Pelham H. R. ERD2, a yeast gene required for the receptor-mediated retrieval of luminal ER proteins from the secretory pathway. Cell. 1990 Jun 29;61(7):1349–1357. doi: 10.1016/0092-8674(90)90698-e. [DOI] [PubMed] [Google Scholar]
  22. Serafini T., Orci L., Amherdt M., Brunner M., Kahn R. A., Rothman J. E. ADP-ribosylation factor is a subunit of the coat of Golgi-derived COP-coated vesicles: a novel role for a GTP-binding protein. Cell. 1991 Oct 18;67(2):239–253. doi: 10.1016/0092-8674(91)90176-y. [DOI] [PubMed] [Google Scholar]
  23. Shimoni Y., Zhu X. Z., Levanony H., Segal G., Galili G. Purification, characterization, and intracellular localization of glycosylated protein disulfide isomerase from wheat grains. Plant Physiol. 1995 May;108(1):327–335. doi: 10.1104/pp.108.1.327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Shorrosh B. S., Dixon R. A. Molecular cloning of a putative plant endomembrane protein resembling vertebrate protein disulfide-isomerase and a phosphatidylinositol-specific phospholipase C. Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10941–10945. doi: 10.1073/pnas.88.23.10941. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Shorrosh B. S., Subramaniam J., Schubert K. R., Dixon R. A. Expression and Localization of Plant Protein Disulfide Isomerase. Plant Physiol. 1993 Nov;103(3):719–726. doi: 10.1104/pp.103.3.719. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Staehelin L. A., Giddings T. H., Jr, Kiss J. Z., Sack F. D. Macromolecular differentiation of Golgi stacks in root tips of Arabidopsis and Nicotiana seedlings as visualized in high pressure frozen and freeze-substituted samples. Protoplasma. 1990;157(1-3):75–91. doi: 10.1007/BF01322640. [DOI] [PubMed] [Google Scholar]
  27. Taylor C. B., Green P. J. Genes with Homology to Fungal and S-Gene RNases Are Expressed in Arabidopsis thaliana. Plant Physiol. 1991 Jul;96(3):980–984. doi: 10.1104/pp.96.3.980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Wilson D. W., Lewis M. J., Pelham H. R. pH-dependent binding of KDEL to its receptor in vitro. J Biol Chem. 1993 Apr 5;268(10):7465–7468. [PubMed] [Google Scholar]

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