Skip to main content
PMC home page
The Plant Cell logoLink to The Plant Cell
. 1995 Jun;7(6):735–745. doi: 10.1105/tpc.7.6.735

LRP1, a gene expressed in lateral and adventitious root primordia of arabidopsis.

D L Smith 1, N V Fedoroff 1
PMCID: PMC160824  PMID: 7647564

Abstract

We describe a gene that is expressed in lateral and adventitious root primordia of Arabidopsis. The gene was identified by expression of a transposon-borne promoterless beta-glucuronidase gene in lateral root primordia. The gene, designated LRP1 for lateral root primordium 1, and its corresponding cDNA were cloned and sequenced. The expression pattern of the gene in lateral root primordia was confirmed by in situ hybridization with LRP1 cDNA probes. The LRP1 gene encodes a novel protein. LRP1 expression is activated during the early stages of root primordium development and is turned off prior to the emergence of lateral roots from the parent root. Insertion of the transposon in the LRP1 gene disrupted its expression. To evaluate the homozygous insertion line for a mutant phenotype, several aspects of wild-type lateral root development were analyzed. A mutant phenotype has not yet been identified in the insertion line; however, there is evidence that the gene belongs to a small gene family. LRP1 provides a molecular marker to study the early stages of lateral and adventitious root primordium development.

Full Text

The Full Text of this article is available as a PDF (3.0 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403–410. doi: 10.1016/S0022-2836(05)80360-2. [DOI] [PubMed] [Google Scholar]
  2. An G., Costa M. A., Ha S. B. Nopaline synthase promoter is wound inducible and auxin inducible. Plant Cell. 1990 Mar;2(3):225–233. doi: 10.1105/tpc.2.3.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Benfey P. N., Schiefelbein J. W. Getting to the root of plant development: the genetics of Arabidopsis root formation. Trends Genet. 1994 Mar;10(3):84–88. doi: 10.1016/0168-9525(94)90230-5. [DOI] [PubMed] [Google Scholar]
  4. Church G. M., Gilbert W. Genomic sequencing. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991–1995. doi: 10.1073/pnas.81.7.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dolan L., Janmaat K., Willemsen V., Linstead P., Poethig S., Roberts K., Scheres B. Cellular organisation of the Arabidopsis thaliana root. Development. 1993 Sep;119(1):71–84. doi: 10.1242/dev.119.1.71. [DOI] [PubMed] [Google Scholar]
  6. Fedoroff N. V., Smith D. L. A versatile system for detecting transposition in Arabidopsis. Plant J. 1993 Feb;3(2):273–289. doi: 10.1111/j.1365-313x.1993.tb00178.x. [DOI] [PubMed] [Google Scholar]
  7. Ferreira P. C., Hemerly A. S., Engler J. D., van Montagu M., Engler G., Inzé D. Developmental expression of the arabidopsis cyclin gene cyc1At. Plant Cell. 1994 Dec;6(12):1763–1774. doi: 10.1105/tpc.6.12.1763. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hemerly A. S., Ferreira P., de Almeida Engler J., Van Montagu M., Engler G., Inzé D. cdc2a expression in Arabidopsis is linked with competence for cell division. Plant Cell. 1993 Dec;5(12):1711–1723. doi: 10.1105/tpc.5.12.1711. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hemerly A., Bergounioux C., Van Montagu M., Inzé D., Ferreira P. Genes regulating the plant cell cycle: isolation of a mitotic-like cyclin from Arabidopsis thaliana. Proc Natl Acad Sci U S A. 1992 Apr 15;89(8):3295–3299. doi: 10.1073/pnas.89.8.3295. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Keller B., Lamb C. J. Specific expression of a novel cell wall hydroxyproline-rich glycoprotein gene in lateral root initiation. Genes Dev. 1989 Oct;3(10):1639–1646. doi: 10.1101/gad.3.10.1639. [DOI] [PubMed] [Google Scholar]
  11. Kopczak S. D., Haas N. A., Hussey P. J., Silflow C. D., Snustad D. P. The small genome of Arabidopsis contains at least six expressed alpha-tubulin genes. Plant Cell. 1992 May;4(5):539–547. doi: 10.1105/tpc.4.5.539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ludwig S. R., Oppenheimer D. G., Silflow C. D., Snustad D. P. Characterization of the alpha-tubulin gene family of Arabidopsis thaliana. Proc Natl Acad Sci U S A. 1987 Aug;84(16):5833–5837. doi: 10.1073/pnas.84.16.5833. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Martinez M. C., Jørgensen J. E., Lawton M. A., Lamb C. J., Doerner P. W. Spatial pattern of cdc2 expression in relation to meristem activity and cell proliferation during plant development. Proc Natl Acad Sci U S A. 1992 Aug 15;89(16):7360–7364. doi: 10.1073/pnas.89.16.7360. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Shapiro M. B., Senapathy P. RNA splice junctions of different classes of eukaryotes: sequence statistics and functional implications in gene expression. Nucleic Acids Res. 1987 Sep 11;15(17):7155–7174. doi: 10.1093/nar/15.17.7155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Springer P. S., McCombie W. R., Sundaresan V., Martienssen R. A. Gene trap tagging of PROLIFERA, an essential MCM2-3-5-like gene in Arabidopsis. Science. 1995 May 12;268(5212):877–880. doi: 10.1126/science.7754372. [DOI] [PubMed] [Google Scholar]

Articles from The Plant Cell are provided here courtesy of Oxford University Press

RESOURCES