Abstract
Changes in host primary metabolism associated with the compatible interaction between cucumber mosaic virus and cotyledons of the marrow plant (Cucurbita pepo L.) have been localized, first by measuring activities of key enzymes in infected and uninfected regions of the cotyledon, and second by histochemical techniques applied to tissue prints of the infected region. A series of progressive metabolic changes occurs within the expanding infected lesion. Virus replication and the synthesis of viral protein at the periphery creates a strong sink demand associated with increased activities of anaplerotic enzymes, increased photosynthesis, and starch accumulation. Inside the lesion, when the synthesis of virus has declined, photosynthesis is reduced, starch is mobilized, and the emphasis of metabolism is shifted toward glycolysis and mitochondrial respiration. These changes are associated spatially with the onset of chlorosis. A decrease in total protein synthesis in this inner zone could be instrumental in some or all of these changes, leading to symptoms of viral infection.
Full Text
The Full Text of this article is available as a PDF (3.2 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Boccard F., Baulcombe D. C. Infectious in vitro transcripts from amplified cDNAs of the Y and Kin strains of cucumber mosaic virus. Gene. 1992 May 15;114(2):223–227. doi: 10.1016/0378-1119(92)90578-d. [DOI] [PubMed] [Google Scholar]
- PLAUT G. W., SUNG S. C. Diphosphopyridine nucleotide isocitric dehydrogenase from animal tissues. J Biol Chem. 1954 Mar;207(1):305–314. [PubMed] [Google Scholar]
- Palukaitis P., Roossinck M. J., Dietzgen R. G., Francki R. I. Cucumber mosaic virus. Adv Virus Res. 1992;41:281–348. doi: 10.1016/s0065-3527(08)60039-1. [DOI] [PubMed] [Google Scholar]
- Rathmell W. G., Sequeira L. Soluble peroxidase in fluid from the intercellular spaces of tobacco leaves. Plant Physiol. 1974 Feb;53(2):317–318. doi: 10.1104/pp.53.2.317. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wang D., Maule A. J. Inhibition of host gene expression associated with plant virus replication. Science. 1995 Jan 13;267(5195):229–231. doi: 10.1126/science.267.5195.229. [DOI] [PubMed] [Google Scholar]