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. 1995 Oct;7(10):1529–1536. doi: 10.1105/tpc.7.10.1529

Expression of the Tomato Pto Gene in Tobacco Enhances Resistance to Pseudomonas syringae pv tabaci Expressing avrPto.

R L Thilmony 1, Z Chen 1, R A Bressan 1, G B Martin 1
PMCID: PMC161003  PMID: 12242354

Abstract

The Pto gene encodes a serine-threonine kinase that confers resistance in tomato to Pseudomonas syringae pv tomato strains expressing the avirulence gene avrPto. We examined the ability of Pto to function in tobacco, a species that is sexually incompatible with tomato. Evidence that a heterologous Pto-like signal transduction pathway is present in tobacco was suggested by the fact that tobacco line Wisconsin-38 exhibits a hypersensitive response after infection with P. syringae pv tabaci expressing avrPto. We introduced a Pto transgene into cultivar Wisconsin-38 and assessed the ability of transformed plants to further inhibit growth of the P. s. tabaci strain expressing avrPto. The Pto-transformed tobacco plants exhibited a significant increase in resistance to the avirulent P. s. tabaci strain compared with wild-type tobacco as indicated by (1) more rapid development of a hypersensitive resistance response at high inoculum concentrations (108 colony-forming units per mL); (2) lessened severity of disease symptoms at moderate inoculum concentrations (106 and 107 colony-forming units per mL); and (3) reduced growth of avirulent P. s. tabaci in inoculated leaves. The results indicate that essential components of a Pto-mediated signal transduction pathway are conserved in tobacco and should prompt examination of resistance gene function across even broader taxonomic distances.

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Selected References

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  1. A simple and general method for transferring genes into plants. Science. 1985 Mar 8;227(4691):1229–1231. doi: 10.1126/science.227.4691.1229. [DOI] [PubMed] [Google Scholar]
  2. Bent A. F., Kunkel B. N., Dahlbeck D., Brown K. L., Schmidt R., Giraudat J., Leung J., Staskawicz B. J. RPS2 of Arabidopsis thaliana: a leucine-rich repeat class of plant disease resistance genes. Science. 1994 Sep 23;265(5180):1856–1860. doi: 10.1126/science.8091210. [DOI] [PubMed] [Google Scholar]
  3. Carland F. M., Staskawicz B. J. Genetic characterization of the Pto locus of tomato: semi-dominance and cosegregation of resistance to Pseudomonas syringae pathovar tomato and sensitivity to the insecticide Fenthion. Mol Gen Genet. 1993 May;239(1-2):17–27. doi: 10.1007/BF00281596. [DOI] [PubMed] [Google Scholar]
  4. Dangl J. L., Ritter C., Gibbon M. J., Mur L. A., Wood J. R., Goss S., Mansfield J., Taylor J. D., Vivian A. Functional homologs of the Arabidopsis RPM1 disease resistance gene in bean and pea. Plant Cell. 1992 Nov;4(11):1359–1369. doi: 10.1105/tpc.4.11.1359. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dangl J. L. The enigmatic avirulence genes of phytopathogenic bacteria. Curr Top Microbiol Immunol. 1994;192:99–118. doi: 10.1007/978-3-642-78624-2_5. [DOI] [PubMed] [Google Scholar]
  6. KLEMENT Z. RAPID DETECTION OF THE PATHOGENICITY OF PHYTOPATHOGENIC PSEUDOMONADS. Nature. 1963 Jul 20;199:299–300. doi: 10.1038/199299b0. [DOI] [PubMed] [Google Scholar]
  7. Keen N. T. Gene-for-gene complementarity in plant-pathogen interactions. Annu Rev Genet. 1990;24:447–463. doi: 10.1146/annurev.ge.24.120190.002311. [DOI] [PubMed] [Google Scholar]
  8. Loh Y. T., Martin G. B. The Pto bacterial resistance gene and the Fen insecticide sensitivity gene encode functional protein kinases with serine/threonine specificity. Plant Physiol. 1995 Aug;108(4):1735–1739. doi: 10.1104/pp.108.4.1735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Martin G. B., Brommonschenkel S. H., Chunwongse J., Frary A., Ganal M. W., Spivey R., Wu T., Earle E. D., Tanksley S. D. Map-based cloning of a protein kinase gene conferring disease resistance in tomato. Science. 1993 Nov 26;262(5138):1432–1436. doi: 10.1126/science.7902614. [DOI] [PubMed] [Google Scholar]
  10. Mindrinos M., Katagiri F., Yu G. L., Ausubel F. M. The A. thaliana disease resistance gene RPS2 encodes a protein containing a nucleotide-binding site and leucine-rich repeats. Cell. 1994 Sep 23;78(6):1089–1099. doi: 10.1016/0092-8674(94)90282-8. [DOI] [PubMed] [Google Scholar]
  11. Moffat A. S. Plant genetics. Mapping the sequence of disease resistance. Science. 1994 Sep 23;265(5180):1804–1805. doi: 10.1126/science.8091208. [DOI] [PubMed] [Google Scholar]
  12. Rommens C. M., Salmeron J. M., Baulcombe D. C., Staskawicz B. J. Use of a gene expression system based on potato virus X to rapidly identify and characterize a tomato Pto homolog that controls fenthion sensitivity. Plant Cell. 1995 Mar;7(3):249–257. doi: 10.1105/tpc.7.3.249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rommens C. M., Salmeron J. M., Oldroyd G. E., Staskawicz B. J. Intergeneric transfer and functional expression of the tomato disease resistance gene Pto. Plant Cell. 1995 Oct;7(10):1537–1544. doi: 10.1105/tpc.7.10.1537. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ronald P. C., Salmeron J. M., Carland F. M., Staskawicz B. J. The cloned avirulence gene avrPto induces disease resistance in tomato cultivars containing the Pto resistance gene. J Bacteriol. 1992 Mar;174(5):1604–1611. doi: 10.1128/jb.174.5.1604-1611.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Salmeron J. M., Barker S. J., Carland F. M., Mehta A. Y., Staskawicz B. J. Tomato mutants altered in bacterial disease resistance provide evidence for a new locus controlling pathogen recognition. Plant Cell. 1994 Apr;6(4):511–520. doi: 10.1105/tpc.6.4.511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Whalen M. C., Innes R. W., Bent A. F., Staskawicz B. J. Identification of Pseudomonas syringae pathogens of Arabidopsis and a bacterial locus determining avirulence on both Arabidopsis and soybean. Plant Cell. 1991 Jan;3(1):49–59. doi: 10.1105/tpc.3.1.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Whalen M. C., Stall R. E., Staskawicz B. J. Characterization of a gene from a tomato pathogen determining hypersensitive resistance in non-host species and genetic analysis of this resistance in bean. Proc Natl Acad Sci U S A. 1988 Sep;85(18):6743–6747. doi: 10.1073/pnas.85.18.6743. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Whitham S., Dinesh-Kumar S. P., Choi D., Hehl R., Corr C., Baker B. The product of the tobacco mosaic virus resistance gene N: similarity to toll and the interleukin-1 receptor. Cell. 1994 Sep 23;78(6):1101–1115. doi: 10.1016/0092-8674(94)90283-6. [DOI] [PubMed] [Google Scholar]

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