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. 1995 Oct;7(10):1537–1544. doi: 10.1105/tpc.7.10.1537

Intergeneric transfer and functional expression of the tomato disease resistance gene Pto.

C M Rommens 1, J M Salmeron 1, G E Oldroyd 1, B J Staskawicz 1
PMCID: PMC161005  PMID: 7580250

Abstract

Plant disease resistance loci have been used successfully in breeding programs to transfer traits from resistant germplasm to susceptible plant cultivars. The molecular cloning of plant disease resistance genes now permits the transfer of such traits across species boundaries by genetic transformation of recipient hosts. The tomato disease resistance gene Pto confers resistance to strains of the bacterial pathogen Pseudomonas syringae pv tomato expressing the avirulence gene avrPto. Transformation of Nicotiana benthamiana with Pto results in specific resistance to P. s. pv tabaci strains carrying avrPto. The resistant phenotype is manifested by a strong inhibition of bacterial growth and the ability to exhibit a hypersensitive response. Resistance cosegregates with the Pto gene in transgene selfings and testcrosses. Our results demonstrate the conservation of disease resistance functions across genus boundaries and suggest that the utility of host-specific resistance genes can be extended by intergeneric transfer.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bent A. F., Kunkel B. N., Dahlbeck D., Brown K. L., Schmidt R., Giraudat J., Leung J., Staskawicz B. J. RPS2 of Arabidopsis thaliana: a leucine-rich repeat class of plant disease resistance genes. Science. 1994 Sep 23;265(5180):1856–1860. doi: 10.1126/science.8091210. [DOI] [PubMed] [Google Scholar]
  2. Carland F. M., Staskawicz B. J. Genetic characterization of the Pto locus of tomato: semi-dominance and cosegregation of resistance to Pseudomonas syringae pathovar tomato and sensitivity to the insecticide Fenthion. Mol Gen Genet. 1993 May;239(1-2):17–27. doi: 10.1007/BF00281596. [DOI] [PubMed] [Google Scholar]
  3. Ellis J. G., Lawrence G. J., Finnegan E. J., Anderson P. A. Contrasting complexity of two rust resistance loci in flax. Proc Natl Acad Sci U S A. 1995 May 9;92(10):4185–4188. doi: 10.1073/pnas.92.10.4185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Figurski D. H., Helinski D. R. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1648–1652. doi: 10.1073/pnas.76.4.1648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jones D. A., Thomas C. M., Hammond-Kosack K. E., Balint-Kurti P. J., Jones J. D. Isolation of the tomato Cf-9 gene for resistance to Cladosporium fulvum by transposon tagging. Science. 1994 Nov 4;266(5186):789–793. doi: 10.1126/science.7973631. [DOI] [PubMed] [Google Scholar]
  6. Jones J. D., Shlumukov L., Carland F., English J., Scofield S. R., Bishop G. J., Harrison K. Effective vectors for transformation, expression of heterologous genes, and assaying transposon excision in transgenic plants. Transgenic Res. 1992 Nov;1(6):285–297. doi: 10.1007/BF02525170. [DOI] [PubMed] [Google Scholar]
  7. Kearney B., Staskawicz B. J. Widespread distribution and fitness contribution of Xanthomonas campestris avirulence gene avrBs2. Nature. 1990 Jul 26;346(6282):385–386. doi: 10.1038/346385a0. [DOI] [PubMed] [Google Scholar]
  8. Klimyuk V. I., Carroll B. J., Thomas C. M., Jones J. D. Alkali treatment for rapid preparation of plant material for reliable PCR analysis. Plant J. 1993 Mar;3(3):493–494. doi: 10.1111/j.1365-313x.1993.tb00169.x. [DOI] [PubMed] [Google Scholar]
  9. Kunkel B. N., Bent A. F., Dahlbeck D., Innes R. W., Staskawicz B. J. RPS2, an Arabidopsis disease resistance locus specifying recognition of Pseudomonas syringae strains expressing the avirulence gene avrRpt2. Plant Cell. 1993 Aug;5(8):865–875. doi: 10.1105/tpc.5.8.865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Loh Y. T., Martin G. B. The Pto bacterial resistance gene and the Fen insecticide sensitivity gene encode functional protein kinases with serine/threonine specificity. Plant Physiol. 1995 Aug;108(4):1735–1739. doi: 10.1104/pp.108.4.1735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Martin G. B., Brommonschenkel S. H., Chunwongse J., Frary A., Ganal M. W., Spivey R., Wu T., Earle E. D., Tanksley S. D. Map-based cloning of a protein kinase gene conferring disease resistance in tomato. Science. 1993 Nov 26;262(5138):1432–1436. doi: 10.1126/science.7902614. [DOI] [PubMed] [Google Scholar]
  12. Martin G. B., Frary A., Wu T., Brommonschenkel S., Chunwongse J., Earle E. D., Tanksley S. D. A member of the tomato Pto gene family confers sensitivity to fenthion resulting in rapid cell death. Plant Cell. 1994 Nov;6(11):1543–1552. doi: 10.1105/tpc.6.11.1543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mindrinos M., Katagiri F., Yu G. L., Ausubel F. M. The A. thaliana disease resistance gene RPS2 encodes a protein containing a nucleotide-binding site and leucine-rich repeats. Cell. 1994 Sep 23;78(6):1089–1099. doi: 10.1016/0092-8674(94)90282-8. [DOI] [PubMed] [Google Scholar]
  14. Rommens C. M., Salmeron J. M., Baulcombe D. C., Staskawicz B. J. Use of a gene expression system based on potato virus X to rapidly identify and characterize a tomato Pto homolog that controls fenthion sensitivity. Plant Cell. 1995 Mar;7(3):249–257. doi: 10.1105/tpc.7.3.249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Salmeron J. M., Barker S. J., Carland F. M., Mehta A. Y., Staskawicz B. J. Tomato mutants altered in bacterial disease resistance provide evidence for a new locus controlling pathogen recognition. Plant Cell. 1994 Apr;6(4):511–520. doi: 10.1105/tpc.6.4.511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Salmeron J. M., Staskawicz B. J. Molecular characterization and hrp dependence of the avirulence gene avrPto from Pseudomonas syringae pv. tomato [corrected]. Mol Gen Genet. 1993 May;239(1-2):6–16. doi: 10.1007/BF00281595. [DOI] [PubMed] [Google Scholar]
  17. Whalen M. C., Stall R. E., Staskawicz B. J. Characterization of a gene from a tomato pathogen determining hypersensitive resistance in non-host species and genetic analysis of this resistance in bean. Proc Natl Acad Sci U S A. 1988 Sep;85(18):6743–6747. doi: 10.1073/pnas.85.18.6743. [DOI] [PMC free article] [PubMed] [Google Scholar]

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