Abstract
Male and female flowers of the dioecious plant sorrel (Rumex acetosa) each produce three whorls of developed floral organs: two similar whorls of three perianth segments and either six stamens (in the male) or a gynoecium consisting of a fertile carpel and two sterile carpels (in the female). In the developing male flower, there is no significant proliferation of cells in the center of the flower, in the position normally occupied by the carpels of a hermaphrodite plant. In the female flower, small stamen primordia are formed. To determine whether the organ differences are associated with differences in the expression of organ identity genes, cDNA clones representing the putative homologs of B and C function MADS box genes were isolated and used in an in situ hybridization analysis. The expression of RAD1 and RAD2 (two different DEFICIENS homologs) in males and females was confined to the stamen whorl; the lack of expression in the second, inner perianth whorl correlated with the sepaloid nature of the inner whorl of perianth segments. Expression of RAP1 (a PLENA homolog) occurred in the carpel and stamen whorls in very young flower primordia from both males and females. However, as soon as the inappropriate set of organs ceased to develop, RAP1 expression became undetectable in those organs. The absence of expression of RAP1 may be the cause of the arrest in organ development or may be a consequence.
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- Angenent G. C., Busscher M., Franken J., Dons H. J., van Tunen A. J. Functional interaction between the homeotic genes fbp1 and pMADS1 during petunia floral organogenesis. Plant Cell. 1995 May;7(5):507–516. doi: 10.1105/tpc.7.5.507. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Coen E. S., Meyerowitz E. M. The war of the whorls: genetic interactions controlling flower development. Nature. 1991 Sep 5;353(6339):31–37. doi: 10.1038/353031a0. [DOI] [PubMed] [Google Scholar]
- Coen E. S., Romero J. M., Doyle S., Elliott R., Murphy G., Carpenter R. floricaula: a homeotic gene required for flower development in antirrhinum majus. Cell. 1990 Dec 21;63(6):1311–1322. doi: 10.1016/0092-8674(90)90426-f. [DOI] [PubMed] [Google Scholar]
- DeLong A., Calderon-Urrea A., Dellaporta S. L. Sex determination gene TASSELSEED2 of maize encodes a short-chain alcohol dehydrogenase required for stage-specific floral organ abortion. Cell. 1993 Aug 27;74(4):757–768. doi: 10.1016/0092-8674(93)90522-r. [DOI] [PubMed] [Google Scholar]
- Goto K., Meyerowitz E. M. Function and regulation of the Arabidopsis floral homeotic gene PISTILLATA. Genes Dev. 1994 Jul 1;8(13):1548–1560. doi: 10.1101/gad.8.13.1548. [DOI] [PubMed] [Google Scholar]
- Gustafson-Brown C., Savidge B., Yanofsky M. F. Regulation of the arabidopsis floral homeotic gene APETALA1. Cell. 1994 Jan 14;76(1):131–143. doi: 10.1016/0092-8674(94)90178-3. [DOI] [PubMed] [Google Scholar]
- Huijser P., Klein J., Lönnig W. E., Meijer H., Saedler H., Sommer H. Bracteomania, an inflorescence anomaly, is caused by the loss of function of the MADS-box gene squamosa in Antirrhinum majus. EMBO J. 1992 Apr;11(4):1239–1249. doi: 10.1002/j.1460-2075.1992.tb05168.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jack T., Brockman L. L., Meyerowitz E. M. The homeotic gene APETALA3 of Arabidopsis thaliana encodes a MADS box and is expressed in petals and stamens. Cell. 1992 Feb 21;68(4):683–697. doi: 10.1016/0092-8674(92)90144-2. [DOI] [PubMed] [Google Scholar]
- Schwarz-Sommer Z., Huijser P., Nacken W., Saedler H., Sommer H. Genetic Control of Flower Development by Homeotic Genes in Antirrhinum majus. Science. 1990 Nov 16;250(4983):931–936. doi: 10.1126/science.250.4983.931. [DOI] [PubMed] [Google Scholar]
- Smyth D. R., Bowman J. L., Meyerowitz E. M. Early flower development in Arabidopsis. Plant Cell. 1990 Aug;2(8):755–767. doi: 10.1105/tpc.2.8.755. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tröbner W., Ramirez L., Motte P., Hue I., Huijser P., Lönnig W. E., Saedler H., Sommer H., Schwarz-Sommer Z. GLOBOSA: a homeotic gene which interacts with DEFICIENS in the control of Antirrhinum floral organogenesis. EMBO J. 1992 Dec;11(13):4693–4704. doi: 10.1002/j.1460-2075.1992.tb05574.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Van Der Krol A. R., Chua N. H. Flower Development in Petunia. Plant Cell. 1993 Oct;5(10):1195–1203. doi: 10.1105/tpc.5.10.1195. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weigel D., Meyerowitz E. M. The ABCs of floral homeotic genes. Cell. 1994 Jul 29;78(2):203–209. doi: 10.1016/0092-8674(94)90291-7. [DOI] [PubMed] [Google Scholar]