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. 1995 Nov;7(11):1801–1810. doi: 10.1105/tpc.7.11.1801

Isolation and characterization of an Arabidopsis mutant deficient in the thylakoid lipid digalactosyl diacylglycerol.

P Dörmann 1, S Hoffmann-Benning 1, I Balbo 1, C Benning 1
PMCID: PMC161039  PMID: 8535135

Abstract

The galactolipids monogalactosyl and digalactosyl diacylglycerol occur in all higher plants and are the predominant lipid components of chloroplast membranes. They are thought to be of major importance to chloroplast morphology and physiology, although direct experimental evidence is still lacking. The enzymes responsible for final assembly of galactolipids are associated with the envelope membranes of plastids, and their biochemical analysis has been notoriously difficult. Therefore, we have chosen a genetic approach to study the biosynthesis and function of galactolipids in higher plants. We isolated a mutant of Arabidopsis that is deficient in digalactosyl diacylglycerol by directly screening a mutagenized M2 population for individuals with altered leaf lipid composition. This mutant carries a recessive nuclear mutation at a single locus designated dgd1. Backcrossed mutants show stunted growth, pale green leaf color, reduced photosynthetic capability, and altered thylakoid membrane ultrastructure.

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Selected References

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  1. Andersson B., Anderson J. M., Ryrie I. J. Transbilayer organization of the chlorophyll-proteins of spinach thylakoids. Eur J Biochem. 1982 Apr 1;123(2):465–472. doi: 10.1111/j.1432-1033.1982.tb19790.x. [DOI] [PubMed] [Google Scholar]
  2. Arondel V., Benning C., Somerville C. R. Isolation and functional expression in Escherichia coli of a gene encoding phosphatidylethanolamine methyltransferase (EC 2.1.1.17) from Rhodobacter sphaeroides. J Biol Chem. 1993 Jul 25;268(21):16002–16008. [PubMed] [Google Scholar]
  3. Bell C. J., Ecker J. R. Assignment of 30 microsatellite loci to the linkage map of Arabidopsis. Genomics. 1994 Jan 1;19(1):137–144. doi: 10.1006/geno.1994.1023. [DOI] [PubMed] [Google Scholar]
  4. Benning C., Somerville C. R. Isolation and genetic complementation of a sulfolipid-deficient mutant of Rhodobacter sphaeroides. J Bacteriol. 1992 Apr;174(7):2352–2360. doi: 10.1128/jb.174.7.2352-2360.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Block M. A., Dorne A. J., Joyard J., Douce R. Preparation and characterization of membrane fractions enriched in outer and inner envelope membranes from spinach chloroplasts. II. Biochemical characterization. J Biol Chem. 1983 Nov 10;258(21):13281–13286. [PubMed] [Google Scholar]
  6. Cline K., Keegstra K. Galactosyltransferases involved in galactolipid biosynthesis are located in the outer membrane of pea chloroplast envelopes. Plant Physiol. 1983 Feb;71(2):366–372. doi: 10.1104/pp.71.2.366. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Heemskerk J. W., Storz T., Schmidt R. R., Heinz E. Biosynthesis of digalactosyldiacylglycerol in plastids from 16:3 and 18:3 plants. Plant Physiol. 1990 Aug;93(4):1286–1294. doi: 10.1104/pp.93.4.1286. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hugly S., Kunst L., Browse J., Somerville C. Enhanced thermal tolerance of photosynthesis and altered chloroplast ultrastructure in a mutant of Arabidopsis deficient in lipid desaturation. Plant Physiol. 1989 Jul;90(3):1134–1142. doi: 10.1104/pp.90.3.1134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Konieczny A., Ausubel F. M. A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers. Plant J. 1993 Aug;4(2):403–410. doi: 10.1046/j.1365-313x.1993.04020403.x. [DOI] [PubMed] [Google Scholar]
  10. Kunst L., Browse J., Somerville C. Altered regulation of lipid biosynthesis in a mutant of Arabidopsis deficient in chloroplast glycerol-3-phosphate acyltransferase activity. Proc Natl Acad Sci U S A. 1988 Jun;85(12):4143–4147. doi: 10.1073/pnas.85.12.4143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kühlbrandt W., Wang D. N. Three-dimensional structure of plant light-harvesting complex determined by electron crystallography. Nature. 1991 Mar 14;350(6314):130–134. doi: 10.1038/350130a0. [DOI] [PubMed] [Google Scholar]
  12. McCourt P., Browse J., Watson J., Arntzen C. J., Somerville C. R. Analysis of Photosynthetic Antenna Function in a Mutant of Arabidopsis thaliana (L.) Lacking trans-Hexadecenoic Acid. Plant Physiol. 1985 Aug;78(4):853–858. doi: 10.1104/pp.78.4.853. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Moffatt B., Somerville C. Positive selection for male-sterile mutants of Arabidopsis lacking adenine phosphoribosyl transferase activity. Plant Physiol. 1988 Apr;86(4):1150–1154. doi: 10.1104/pp.86.4.1150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nussberger S., Dörr K., Wang D. N., Kühlbrandt W. Lipid-protein interactions in crystals of plant light-harvesting complex. J Mol Biol. 1993 Nov 20;234(2):347–356. doi: 10.1006/jmbi.1993.1591. [DOI] [PubMed] [Google Scholar]
  15. Roughan P. G. Turnover of the glycerolipids of pumpkin leaves. The importence of phosphatidylcholine. Biochem J. 1970 Mar;117(1):1–8. doi: 10.1042/bj1170001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. SASTRY P. S., KATES M. HYDROLYSIS OF MONOGALACTOSYL AND DIGALACTOSYL DIGLYCERIDES BY SPECIFIC ENZYMES IN RUNNER-BEAN LEAVES. Biochemistry. 1964 Sep;3:1280–1287. doi: 10.1021/bi00897a016. [DOI] [PubMed] [Google Scholar]
  17. Sakaki T., Kondo N., Yamada M. Pathway for the synthesis of triacylglycerols from monogalactosyldiacylglycerols in ozone-fumigated spinach leaves. Plant Physiol. 1990 Oct;94(2):773–780. doi: 10.1104/pp.94.2.773. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Torisu M., Hayashi Y., Ishimitsu T., Fujimura T., Iwasaki K., Katano M., Yamamoto H., Kimura Y., Takesue M., Kondo M. Significant prolongation of disease-free period gained by oral polysaccharide K (PSK) administration after curative surgical operation of colorectal cancer. Cancer Immunol Immunother. 1990;31(5):261–268. doi: 10.1007/BF01740932. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wintermans J. F., Helmsing P. J., Polman B. J., van Gisbergen J., Collard J. Galactolipid transformations and photochemical activities of spinach chloroplasts. Biochim Biophys Acta. 1969 Sep 16;189(1):95–105. doi: 10.1016/0005-2728(69)90230-8. [DOI] [PubMed] [Google Scholar]
  20. van Besouw A., Wintermans J. F. Galactolipid formation in chloroplast envelopes. I. Evidence for two mechanisms in galactosylation. Biochim Biophys Acta. 1978 Apr 28;529(1):44–53. doi: 10.1016/0005-2760(78)90102-9. [DOI] [PubMed] [Google Scholar]

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