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. 1996 Mar;8(3):477–487. doi: 10.1105/tpc.8.3.477

Oligogalacturonides Prevent Rhizogenesis in rolB-Transformed Tobacco Explants by Inhibiting Auxin-Induced Expression of the rolB Gene.

D Bellincampi 1, M Cardarelli 1, D Zaghi 1, G Serino 1, G Salvi 1, C Gatz 1, F Cervone 1, MM Altamura 1, P Costantino 1, GD Lorenzo 1
PMCID: PMC161114  PMID: 12239391

Abstract

Oligogalacturonides elicit several defense responses and regulate different aspects of growth and development in plants. Many of the development-related effects of oligogalacturonides appear to be amenable to an auxin antagonist activity of these oligosaccharins. To clarify the role of oligogalacturonides in antagonizing auxin, we analyzed their effect on root formation in leaf explants of tobacco harboring the plant oncogene rolB. We show here that oligogalacturonides are capable of inhibiting root morphogenesis driven by rolB in transgenic leaf explants when this process requires exogenous auxin. Because rolB expression is induced by auxin and dramatically alters the response to this hormone in transformed plant cells, the inhibiting effect of oligogalacturonides could be exerted on the induction of rolB and/or at some other auxin-requiring step(s) in rhizogenesis. We show that oligogalacturonides antagonize auxin primarily because they strongly inhibit auxin-regulated transcriptional activation of a rolB-[beta]-glucuronidase gene fusion in both leaf explants and cultured leaf protoplasts. In contrast, oligogalacturonides do not inhibit rhizogenesis when rolB transcriptional activation is made independent of auxin, as shown by the lack of inhibition of root formation in leaf explants containing rolB driven by a tetracycline-inducible promoter.

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Selected References

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  1. Apostol I., Heinstein P. F., Low P. S. Rapid Stimulation of an Oxidative Burst during Elicitation of Cultured Plant Cells : Role in Defense and Signal Transduction. Plant Physiol. 1989 May;90(1):109–116. doi: 10.1104/pp.90.1.109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bergmann C. W., Ito Y., Singer D., Albersheim P., Darvill A. G., Benhamou N., Nuss L., Salvi G., Cervone F., De Lorenzo G. Polygalacturonase-inhibiting protein accumulates in Phaseolus vulgaris L. in response to wounding, elicitors and fungal infection. Plant J. 1994 May;5(5):625–634. doi: 10.1111/j.1365-313x.1994.00625.x. [DOI] [PubMed] [Google Scholar]
  3. Capone I., Cardarelli M., Mariotti D., Pomponi M., De Paolis A., Costantino P. Different promoter regions control level and tissue specificity of expression of Agrobacterium rhizogenes rolB gene in plants. Plant Mol Biol. 1991 Mar;16(3):427–436. doi: 10.1007/BF00023993. [DOI] [PubMed] [Google Scholar]
  4. Capone I., Spanò L., Cardarelli M., Bellincampi D., Petit A., Costantino P. Induction and growth properties of carrot roots with different complements of Agrobacterium rhizogenes T-DNA. Plant Mol Biol. 1989 Jul;13(1):43–52. doi: 10.1007/BF00027334. [DOI] [PubMed] [Google Scholar]
  5. Chandra S., Low P. S. Role of phosphorylation in elicitation of the oxidative burst in cultured soybean cells. Proc Natl Acad Sci U S A. 1995 May 9;92(10):4120–4123. doi: 10.1073/pnas.92.10.4120. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Côté F., Hahn M. G. Oligosaccharins: structures and signal transduction. Plant Mol Biol. 1994 Dec;26(5):1379–1411. doi: 10.1007/BF00016481. [DOI] [PubMed] [Google Scholar]
  7. Darvill A., Augur C., Bergmann C., Carlson R. W., Cheong J. J., Eberhard S., Hahn M. G., Ló V. M., Marfà V., Meyer B. Oligosaccharins--oligosaccharides that regulate growth, development and defence responses in plants. Glycobiology. 1992 Jun;2(3):181–198. doi: 10.1093/glycob/2.3.181. [DOI] [PubMed] [Google Scholar]
  8. Filippini F., Rossi V., Marin O., Trovato M., Costantino P., Downey P. M., Lo Schiavo F., Terzi M. A plant oncogene as a phosphatase. Nature. 1996 Feb 8;379(6565):499–500. doi: 10.1038/379499a0. [DOI] [PubMed] [Google Scholar]
  9. Legendre L., Heinstein P. F., Low P. S. Evidence for participation of GTP-binding proteins in elicitation of the rapid oxidative burst in cultured soybean cells. J Biol Chem. 1992 Oct 5;267(28):20140–20147. [PubMed] [Google Scholar]
  10. Legendre L., Rueter S., Heinstein P. F., Low P. S. Characterization of the Oligogalacturonide-Induced Oxidative Burst in Cultured Soybean (Glycine max) Cells. Plant Physiol. 1993 May;102(1):233–240. doi: 10.1104/pp.102.1.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Maliga P., Sz-Breznovits A., Márton L. Streptomycin-resistant plants from callus culture of haploid tobacco. Nat New Biol. 1973 Jul 4;244(131):29–30. doi: 10.1038/newbio244029a0. [DOI] [PubMed] [Google Scholar]
  12. Maurel C., Brevet J., Barbier-Brygoo H., Guern J., Tempé J. Auxin regulates the promoter of the root-inducing rolB gene of Agrobacterium rhizogenes in transgenic tobacco. Mol Gen Genet. 1990 Aug;223(1):58–64. doi: 10.1007/BF00315797. [DOI] [PubMed] [Google Scholar]
  13. Reymond P., Grünberger S., Paul K., Müller M., Farmer E. E. Oligogalacturonide defense signals in plants: large fragments interact with the plasma membrane in vitro. Proc Natl Acad Sci U S A. 1995 May 9;92(10):4145–4149. doi: 10.1073/pnas.92.10.4145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Röder F. T., Schmülling T., Gatz C. Efficiency of the tetracycline-dependent gene expression system: complete suppression and efficient induction of the rolB phenotype in transgenic plants. Mol Gen Genet. 1994 Apr;243(1):32–38. doi: 10.1007/BF00283873. [DOI] [PubMed] [Google Scholar]
  15. Spanò L., Mariotti D., Cardarelli M., Branca C., Costantino P. Morphogenesis and Auxin Sensitivity of Transgenic Tobacco with Different Complements of Ri T-DNA. Plant Physiol. 1988 Jun;87(2):479–483. doi: 10.1104/pp.87.2.479. [DOI] [PMC free article] [PubMed] [Google Scholar]

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