Skip to main content
NCBI home page
The Plant Cell logoLink to The Plant Cell
. 1996 May;8(5):823–830. doi: 10.1105/tpc.8.5.823

Localization of T-DNA Insertions in Petunia by Fluorescence in Situ Hybridization: Physical Evidence for Suppression of Recombination.

R Ten Hoopen 1, T P Robbins 1, P F Fransz 1, B M Montijn 1, O Oud 1, AGM Gerats 1, N Nanninga 1
PMCID: PMC161141  PMID: 12239403

Abstract

Using fluorescence in situ hybridization (FISH) with metaphase preparations, we localized a 4-kb single-copy T-DNA sequence in a group of petunia transformants. The selected T-DNAs previously had been shown to be linked to the phenotypic marker FI on chromosome II. Linkage analysis had revealed that recombination around the FI locus is suppressed in a wide cross relative to an inbred recombination assay. The localization of six FI-linked T-DNAs and the FI locus itself, using FISH, revealed a number of aspects of recombination in petunia: (1) the central region of chromosome II showed at least a 10-fold suppression of recombination in wide crosses relative to the distal region; (2) recombination in wide hybrids over two-thirds of the chromosome was extremely low; and (3) recombination between completely homologous chromosomes in an inbred cross also was suppressed in the central region. In addition, the T-DNAs were not evenly distributed along the chromosome, suggesting a possible preference for a distal position for T-DNA integration. Implications for such a preference are discussed.

Full Text

The Full Text of this article is available as a PDF (2.0 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ambros P. F., Matzke A. J. M., Matzke M. A. Localization of Agrobacterium rhizogenes T-DNA in plant chromosomes by in situ hybridization. EMBO J. 1986 Sep;5(9):2073–2077. doi: 10.1002/j.1460-2075.1986.tb04468.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dennis P. P. Multiple promoters for the transcription of the ribosomal RNA gene cluster in Halobacterium cutirubrum. J Mol Biol. 1985 Nov 20;186(2):457–461. doi: 10.1016/0022-2836(85)90117-2. [DOI] [PubMed] [Google Scholar]
  3. Ingelbrecht I., Breyne P., Vancompernolle K., Jacobs A., Van Montagu M., Depicker A. Transcriptional interference in transgenic plants. Gene. 1991 Dec 30;109(2):239–242. doi: 10.1016/0378-1119(91)90614-h. [DOI] [PubMed] [Google Scholar]
  4. Jones J. D., Carland F. M., Maliga P., Dooner H. K. Visual detection of transposition of the maize element activator (ac) in tobacco seedlings. Science. 1989 Apr 14;244(4901):204–207. doi: 10.1126/science.244.4901.204. [DOI] [PubMed] [Google Scholar]
  5. Koukolíková-Nicola Z., Hohn B. How does the T-DNA of Agrobacterium tumefaciens find its way into the plant cell nucleus? Biochimie. 1993;75(8):635–638. doi: 10.1016/0300-9084(93)90092-7. [DOI] [PubMed] [Google Scholar]
  6. Mayerhofer R., Koncz-Kalman Z., Nawrath C., Bakkeren G., Crameri A., Angelis K., Redei G. P., Schell J., Hohn B., Koncz C. T-DNA integration: a mode of illegitimate recombination in plants. EMBO J. 1991 Mar;10(3):697–704. doi: 10.1002/j.1460-2075.1991.tb07999.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. ROBERTS P. A. DIFFERENCE IN THE BEHAVIOR OF EU- AND HETERO-CHROMATIN: CROSSING-OVER. Nature. 1965 Feb 13;205:725–726. doi: 10.1038/205725b0. [DOI] [PubMed] [Google Scholar]
  8. Rose A. M., Baillie D. L. A mutation in Caenorhabditis elegans that increases recombination frequency more than threefold. Nature. 1979 Oct 18;281(5732):599–600. doi: 10.1038/281599a0. [DOI] [PubMed] [Google Scholar]
  9. Thomas C. M., Jones D. A., English J. J., Carroll B. J., Bennetzen J. L., Harrison K., Burbidge A., Bishop G. J., Jones J. D. Analysis of the chromosomal distribution of transposon-carrying T-DNAs in tomato using the inverse polymerase chain reaction. Mol Gen Genet. 1994 Mar;242(5):573–585. doi: 10.1007/BF00285281. [DOI] [PubMed] [Google Scholar]

Articles from The Plant Cell are provided here courtesy of Oxford University Press

RESOURCES