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. 1996 Oct;8(10):1845–1853. doi: 10.1105/tpc.8.10.1845

Using Hypoviruses to Probe and Perturb Signal Transduction Processes Underlying Fungal Pathogenesis.

DL Nuss 1
PMCID: PMC161319  PMID: 12239366

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anagnostakis S. L. Biological control of chestnut blight. Science. 1982 Jan 29;215(4532):466–471. doi: 10.1126/science.215.4532.466. [DOI] [PubMed] [Google Scholar]
  2. Baulcombe D. C. Mechanisms of Pathogen-Derived Resistance to Viruses in Transgenic Plants. Plant Cell. 1996 Oct;8(10):1833–1844. doi: 10.1105/tpc.8.10.1833. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chen B., Craven M. G., Choi G. H., Nuss D. L. cDNA-derived hypovirus RNA in transformed chestnut blight fungus is spliced and trimmed of vector nucleotides. Virology. 1994 Jul;202(1):441–448. doi: 10.1006/viro.1994.1360. [DOI] [PubMed] [Google Scholar]
  4. Chen B., Gao S., Choi G. H., Nuss D. L. Extensive alteration of fungal gene transcript accumulation and elevation of G-protein-regulated cAMP levels by a virulence-attenuating hypovirus. Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):7996–8000. doi: 10.1073/pnas.93.15.7996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Choi G. H., Chen B., Nuss D. L. Virus-mediated or transgenic suppression of a G-protein alpha subunit and attenuation of fungal virulence. Proc Natl Acad Sci U S A. 1995 Jan 3;92(1):305–309. doi: 10.1073/pnas.92.1.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Choi G. H., Larson T. G., Nuss D. L. Molecular analysis of the laccase gene from the chestnut blight fungus and selective suppression of its expression in an isogenic hypovirulent strain. Mol Plant Microbe Interact. 1992 Mar-Apr;5(2):119–128. doi: 10.1094/mpmi-5-119. [DOI] [PubMed] [Google Scholar]
  7. Choi G. H., Nuss D. L. A viral gene confers hypovirulence-associated traits to the chestnut blight fungus. EMBO J. 1992 Feb;11(2):473–477. doi: 10.1002/j.1460-2075.1992.tb05077.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Choi G. H., Nuss D. L. Hypovirulence of chestnut blight fungus conferred by an infectious viral cDNA. Science. 1992 Aug 7;257(5071):800–803. doi: 10.1126/science.1496400. [DOI] [PubMed] [Google Scholar]
  9. Choi G. H., Pawlyk D. M., Nuss D. L. The autocatalytic protease p29 encoded by a hypovirulence-associated virus of the chestnut blight fungus resembles the potyvirus-encoded protease HC-Pro. Virology. 1991 Aug;183(2):747–752. doi: 10.1016/0042-6822(91)91004-z. [DOI] [PubMed] [Google Scholar]
  10. Choi G. H., Pawlyk D. M., Rae B., Shapira R., Nuss D. L. Molecular analysis and overexpression of the gene encoding endothiapepsin, an aspartic protease from Cryphonectria parasitica. Gene. 1993 Mar 30;125(2):135–141. doi: 10.1016/0378-1119(93)90320-3. [DOI] [PubMed] [Google Scholar]
  11. Choi G. H., Shapira R., Nuss D. L. Cotranslational autoproteolysis involved in gene expression from a double-stranded RNA genetic element associated with hypovirulence of the chestnut blight fungus. Proc Natl Acad Sci U S A. 1991 Feb 15;88(4):1167–1171. doi: 10.1073/pnas.88.4.1167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Craven M. G., Pawlyk D. M., Choi G. H., Nuss D. L. Papain-like protease p29 as a symptom determinant encoded by a hypovirulence-associated virus of the chestnut blight fungus. J Virol. 1993 Nov;67(11):6513–6521. doi: 10.1128/jvi.67.11.6513-6521.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Fahima T., Kazmierczak P., Hansen D. R., Pfeiffer P., Van Alfen N. K. Membrane-associated replication of an unencapsidated double-strand RNA of the fungus, Cryphonectria parasitica. Virology. 1993 Jul;195(1):81–89. doi: 10.1006/viro.1993.1348. [DOI] [PubMed] [Google Scholar]
  14. Fahima T., Wu Y., Zhang L., Van Alfen N. K. Identification of the putative RNA polymerase of Cryphonectria hypovirus in a solubilized replication complex. J Virol. 1994 Sep;68(9):6116–6119. doi: 10.1128/jvi.68.9.6116-6119.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Gao S., Choi G. H., Shain L., Nuss D. L. Cloning and targeted disruption of enpg-1, encoding the major in vitro extracellular endopolygalacturonase of the chestnut blight fungus, Cryphonectria parasitica. Appl Environ Microbiol. 1996 Jun;62(6):1984–1990. doi: 10.1128/aem.62.6.1984-1990.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Gilman A. G. G proteins: transducers of receptor-generated signals. Annu Rev Biochem. 1987;56:615–649. doi: 10.1146/annurev.bi.56.070187.003151. [DOI] [PubMed] [Google Scholar]
  17. Handelsman J., Stabb E. V. Biocontrol of Soilborne Plant Pathogens. Plant Cell. 1996 Oct;8(10):1855–1869. doi: 10.1105/tpc.8.10.1855. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Hillman B. I., Halpern B. T., Brown M. P. A viral dsRNA element of the chestnut blight fungus with a distinct genetic organization. Virology. 1994 Jun;201(2):241–250. doi: 10.1006/viro.1994.1289. [DOI] [PubMed] [Google Scholar]
  19. Hillman B. I., Tian Y., Bedker P. J., Brown M. P. A North American hypovirulent isolate of the chestnut blight fungus with European isolate-related dsRNA. J Gen Virol. 1992 Mar;73(Pt 3):681–686. doi: 10.1099/0022-1317-73-3-681. [DOI] [PubMed] [Google Scholar]
  20. Kazmierczak P., Pfeiffer P., Zhang L., Van Alfen N. K. Transcriptional repression of specific host genes by the mycovirus Cryphonectria hypovirus 1. J Virol. 1996 Feb;70(2):1137–1142. doi: 10.1128/jvi.70.2.1137-1142.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Larson T. G., Choi G. H., Nuss D. L. Regulatory pathways governing modulation of fungal gene expression by a virulence-attenuating mycovirus. EMBO J. 1992 Dec;11(12):4539–4548. doi: 10.1002/j.1460-2075.1992.tb05555.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Lindbo J. A., Dougherty W. G. Untranslatable transcripts of the tobacco etch virus coat protein gene sequence can interfere with tobacco etch virus replication in transgenic plants and protoplasts. Virology. 1992 Aug;189(2):725–733. doi: 10.1016/0042-6822(92)90595-g. [DOI] [PubMed] [Google Scholar]
  23. Polashock J. J., Anagnostakis S. L., Milgroom M. G., Hillman B. I. Isolation and characterization of a virus-resistant mutant of Cryphonectria parasitica. Curr Genet. 1994 Nov-Dec;26(5-6):528–534. doi: 10.1007/BF00309945. [DOI] [PubMed] [Google Scholar]
  24. Powell W. A., Van Alfen N. K. Differential accumulation of poly(A)+ RNA between virulent and double-stranded RNA-induced hypovirulent strains of Cryphonectria (Endothia) parasitica. Mol Cell Biol. 1987 Oct;7(10):3688–3693. doi: 10.1128/mcb.7.10.3688. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Rigling D., Van Alfen N. K. Regulation of laccase biosynthesis in the plant-pathogenic fungus Cryphonectria parasitica by double-stranded RNA. J Bacteriol. 1991 Dec;173(24):8000–8003. doi: 10.1128/jb.173.24.8000-8003.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Shapira R., Nuss D. L. Gene expression by a hypovirulence-associated virus of the chestnut blight fungus involves two papain-like protease activities. Essential residues and cleavage site requirements for p48 autoproteolysis. J Biol Chem. 1991 Oct 15;266(29):19419–19425. [PubMed] [Google Scholar]
  27. Turner G. E., Borkovich K. A. Identification of a G protein alpha subunit from Neurospora crassa that is a member of the Gi family. J Biol Chem. 1993 Jul 15;268(20):14805–14811. [PubMed] [Google Scholar]
  28. Varley D. A., Podila G. K., Hiremath S. T. Cutinase in Cryphonectria parasitica, the chestnut blight fungus: suppression of cutinase gene expression in isogenic hypovirulent strains containing double-stranded RNAs. Mol Cell Biol. 1992 Oct;12(10):4539–4544. doi: 10.1128/mcb.12.10.4539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Zhang L., Churchill A. C., Kazmierczak P., Kim D. H., Van Alfen N. K. Hypovirulence-associated traits induced by a mycovirus of Cryphonectria parasitica are mimicked by targeted inactivation of a host gene. Mol Cell Biol. 1993 Dec;13(12):7782–7792. doi: 10.1128/mcb.13.12.7782. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Zhang L., Villalon D., Sun Y., Kazmierczak P., van Alfen N. K. Virus-associated down-regulation of the gene encoding cryparin, an abundant cell-surface protein from the chestnut blight fungus, Cryphonectria parasitica. Gene. 1994 Feb 11;139(1):59–64. doi: 10.1016/0378-1119(94)90523-1. [DOI] [PubMed] [Google Scholar]

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