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. 1996 Dec;8(12):2211–2221. doi: 10.1105/tpc.8.12.2211

Gibberellic Acid Induces Vacuolar Acidification in Barley Aleurone.

S J Swanson 1, R L Jones 1
PMCID: PMC161346  PMID: 12239377

Abstract

The roles of gibberellic acid (GA3) and abscisic acid (ABA) in the regulation of vacuolar pH (pHv) in aleurone cells of barley were investigated using the pH-sensitive fluorescent dye 2[prime],7[prime]-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF). BCECF accumulated in vacuoles of aleurone cells, but sequestration of the dye did not affect its sensitivity to pH. BCECF-loaded aleurone cells retained their ability to respond to both GA3 and ABA. The pHv of freshly isolated aleurone cells is 6.6, but after incubation in GA3, the pHv fell to 5.8. The pHv of cells not incubated in hormones or in the presence of ABA showed little or no acidification. The aleurone tonoplast contains both vacuolar ATPase and vacuolar pyrophosphatase, but the levels of pump proteins were not affected by incubation in the presence or absence of hormones. We conclude that GA3 affects the pHv in aleurone cells by altering the activities of tonoplast H+ pumps but not the amounts of pump proteins.

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Selected References

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  1. Bethke P. C., Hillmer S., Jones R. L. Isolation of Intact Protein Storage Vacuoles from Barley Aleurone (Identification of Aspartic and Cysteine Proteases). Plant Physiol. 1996 Feb;110(2):521–529. doi: 10.1104/pp.110.2.521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bethke P. C., Jones R. L. Ca2+-Calmodulin Modulates Ion Channel Activity in Storage Protein Vacuoles of Barley Aleurone Cells. Plant Cell. 1994 Feb;6(2):277–285. doi: 10.1105/tpc.6.2.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blumwald E., Poole R. J. Kinetics of Ca/H Antiport in Isolated Tonoplast Vesicles from Storage Tissue of Beta vulgaris L. Plant Physiol. 1986 Mar;80(3):727–731. doi: 10.1104/pp.80.3.727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Blumwald E., Poole R. J. Na/H Antiport in Isolated Tonoplast Vesicles from Storage Tissue of Beta vulgaris. Plant Physiol. 1985 May;78(1):163–167. doi: 10.1104/pp.78.1.163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bush D. S., Cornejo M. J., Huang C. N., Jones R. L. Ca-stimulated secretion of alpha-amylase during development in barley aleurone protoplasts. Plant Physiol. 1986 Oct;82(2):566–574. doi: 10.1104/pp.82.2.566. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Davies J. M., Hunt I., Sanders D. Vacuolar H(+)-pumping ATPase variable transport coupling ratio controlled by pH. Proc Natl Acad Sci U S A. 1994 Aug 30;91(18):8547–8551. doi: 10.1073/pnas.91.18.8547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Feng Y., Forgac M. A novel mechanism for regulation of vacuolar acidification. J Biol Chem. 1992 Oct 5;267(28):19769–19772. [PubMed] [Google Scholar]
  8. Filner P., Varner J. E. A test for de novo synthesis of enzymes: density labeling with H2O18 of barley alpha-amylase induced by gibberellic acid. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1520–1526. doi: 10.1073/pnas.58.4.1520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hillmer S., Gilroy S., Jones R. L. Visualizing Enzyme Secretion from Individual Barley (Hordeum vulgare) Aleurone Protoplasts. Plant Physiol. 1993 May;102(1):279–286. doi: 10.1104/pp.102.1.279. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Holwerda B. C., Rogers J. C. Purification and characterization of aleurain : a plant thiol protease functionally homologous to Mammalian cathepsin h. Plant Physiol. 1992 Jul;99(3):848–855. doi: 10.1104/pp.99.3.848. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jones R. L. Gibberellic Acid and Ion Release from Barley Aleurone Tissue: Evidence for Hormone-dependent Ion Transport Capacity. Plant Physiol. 1973 Oct;52(4):303–308. doi: 10.1104/pp.52.4.303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Maeshima M. H(+)-translocating inorganic pyrophosphatase of plant vacuoles. Inhibition by Ca2+, stabilization by Mg2+ and immunological comparison with other inorganic pyrophosphatases. Eur J Biochem. 1991 Feb 26;196(1):11–17. doi: 10.1111/j.1432-1033.1991.tb15779.x. [DOI] [PubMed] [Google Scholar]
  13. Martiny-Baron G., Manolson M. F., Poole R. J., Hecker D., Scherer G. F. Proton transport and phosphorylation of tonoplast polypeptides from zucchini are stimulated by the phospholipid platelet-activating factor. Plant Physiol. 1992 Aug;99(4):1635–1641. doi: 10.1104/pp.99.4.1635. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Menegus F., Cattaruzza L., Mattana M., Beffagna N., Ragg E. Response to Anoxia in Rice and Wheat Seedlings: Changes in the pH of Intracellular Compartments, Glucose-6-Phosphate Level, and Metabolic Rate. Plant Physiol. 1991 Mar;95(3):760–767. doi: 10.1104/pp.95.3.760. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mundy J., Hejgaard J., Hansen A., Hallgren L., Jorgensen K. G., Munck L. Differential synthesis in vitro of barley aleurone and starchy endosperm proteins. Plant Physiol. 1986 Jun;81(2):630–636. doi: 10.1104/pp.81.2.630. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Müller M., Irkens-Kiesecker U., Rubinstein B., Taiz L. On the mechanism of hyperacidification in lemon. Comparison of the vacuolar H(+)-ATPase activities of fruits and epicotyls. J Biol Chem. 1996 Jan 26;271(4):1916–1924. doi: 10.1074/jbc.271.4.1916. [DOI] [PubMed] [Google Scholar]
  17. Nelson N., Taiz L. The evolution of H+-ATPases. Trends Biochem Sci. 1989 Mar;14(3):113–116. doi: 10.1016/0968-0004(89)90134-5. [DOI] [PubMed] [Google Scholar]
  18. Okita Thomas W., Rogers John C. COMPARTMENTATION OF PROTEINS IN THE ENDOMEMBRANE SYSTEM OF PLANT CELLS. Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47(NaN):327–350. doi: 10.1146/annurev.arplant.47.1.327. [DOI] [PubMed] [Google Scholar]
  19. Rea P. A., Britten C. J., Jennings I. R., Calvert C. M., Skiera L. A., Leigh R. A., Sanders D. Regulation of vacuolar h-pyrophosphatase by free calcium : a reaction kinetic analysis. Plant Physiol. 1992 Dec;100(4):1706–1715. doi: 10.1104/pp.100.4.1706. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Taiz L. THE PLANT VACUOLE. J Exp Biol. 1992 Nov 1;172(Pt 1):113–122. doi: 10.1242/jeb.172.1.113. [DOI] [PubMed] [Google Scholar]

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