Abstract
In Brassica species, self-incompatibility has been mapped genetically to a single chromosomal location. In this region, there are two closely linked genes coding for the S locus glycoprotein (SLG) and S locus receptor kinase (SRK). They appear to comprise the pistil component of the self-incompatibility reaction. SLG and SRK are thought to recognize an unknown pollen component on the incompatible pollen, and the gene encoding this pollen component must also be linked to the SLG and SRK genes. To further our understanding of self-incompatibility, the chromosomal region carrying the SLG and SRK genes has been studied. The physical region between the SLG-910 and the SRK-910 genes in the Brassica napus W1 line was cloned, and a search for genes expressed in the anther revealed two additional S locus genes located downstream of the SLG-910 gene. Because these two genes are novel and are conserved at other S alleles, we designated them as SLL1 and SLL2 (for S locus-linked genes 1 and 2, respectively). The SLL1 gene is S locus specific, whereas the SLL2 gene is not only present at the S locus but is also present in other parts of the genomes in both self-incompatible and self-compatible Brassica ssp lines. Expression of the SLL1 gene is only detectable in anthers of self-incompatible plants and is developmentally regulated during anther development, whereas the SLL2 gene is expressed in anthers and stigmas in both self-incompatible and self-compatible plants, with the highest levels of expression occurring in the stigmas. Although SLL1 and SLL2 are linked to the S locus region, it is not clear whether these genes function in self-incompatibility or serve some other cellular roles in pollen-pistil functions.
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- Boyes D. C., Nasrallah J. B. An anther-specific gene encoded by an S locus haplotype of Brassica produces complementary and differentially regulated transcripts. Plant Cell. 1995 Aug;7(8):1283–1294. doi: 10.1105/tpc.7.8.1283. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Boyes D. C., Nasrallah J. B. Physical linkage of the SLG and SRK genes at the self-incompatibility locus of Brassica oleracea. Mol Gen Genet. 1993 Jan;236(2-3):369–373. doi: 10.1007/BF00277135. [DOI] [PubMed] [Google Scholar]
- Brown C. J., Hendrich B. D., Rupert J. L., Lafrenière R. G., Xing Y., Lawrence J., Willard H. F. The human XIST gene: analysis of a 17 kb inactive X-specific RNA that contains conserved repeats and is highly localized within the nucleus. Cell. 1992 Oct 30;71(3):527–542. doi: 10.1016/0092-8674(92)90520-m. [DOI] [PubMed] [Google Scholar]
- Da Rocha P. S., Bertrand H. Structure and comparative analysis of the rDNA intergenic spacer of Brassica rapa. Implications for the function and evolution of the Cruciferae spacer. Eur J Biochem. 1995 Apr 15;229(2):550–557. doi: 10.1111/j.1432-1033.1995.tb20497.x. [DOI] [PubMed] [Google Scholar]
- Delorme V., Giranton J. L., Hatzfeld Y., Friry A., Heizmann P., Ariza M. J., Dumas C., Gaude T., Cock J. M. Characterization of the S locus genes, SLG and SRK, of the Brassica S3 haplotype: identification of a membrane-localized protein encoded by the S locus receptor kinase gene. Plant J. 1995 Mar;7(3):429–440. doi: 10.1046/j.1365-313x.1995.7030429.x. [DOI] [PubMed] [Google Scholar]
- Dwyer K. G., Balent M. A., Nasrallah J. B., Nasrallah M. E. DNA sequences of self-incompatibility genes from Brassica campestris and B. oleracea: polymorphism predating speciation. Plant Mol Biol. 1991 Mar;16(3):481–486. doi: 10.1007/BF00024000. [DOI] [PubMed] [Google Scholar]
- Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
- Giranton J. L., Ariza M. J., Dumas C., Cock J. M., Gaude T. The S locus receptor kinase gene encodes a soluble glycoprotein corresponding to the SKR extracellular domain in Brassica oleracea. Plant J. 1995 Dec;8(6):827–834. doi: 10.1046/j.1365-313x.1995.8060827.x. [DOI] [PubMed] [Google Scholar]
- Glavin T. L., Goring D. R., Schafer U., Rothstein S. J. Features of the extracellular domain of the S-locus receptor kinase from Brassica. Mol Gen Genet. 1994 Sep 28;244(6):630–637. doi: 10.1007/BF00282753. [DOI] [PubMed] [Google Scholar]
- Goring D. R., Banks P., Beversdorf W. D., Rothstein S. J. Use of the polymerase chain reaction to isolate an S-locus glycoprotein cDNA introgressed from Brassica campestris into B. napus ssp. oleifera. Mol Gen Genet. 1992 Aug;234(2):185–192. doi: 10.1007/BF00283838. [DOI] [PubMed] [Google Scholar]
- Goring D. R., Banks P., Fallis L., Baszczynski C. L., Beversdorf W. D., Rothstein S. J. Identification of an S-locus glycoprotein allele introgressed from B. napus ssp. rapifera to B. napus ssp. oleifera. Plant J. 1992 Nov;2(6):983–989. [PubMed] [Google Scholar]
- Goring D. R., Glavin T. L., Schafer U., Rothstein S. J. An S receptor kinase gene in self-compatible Brassica napus has a 1-bp deletion. Plant Cell. 1993 May;5(5):531–539. doi: 10.1105/tpc.5.5.531. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goring D. R., Rothstein S. J. The S-locus receptor kinase gene in a self-incompatible Brassica napus line encodes a functional serine/threonine kinase. Plant Cell. 1992 Oct;4(10):1273–1281. doi: 10.1105/tpc.4.10.1273. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Johnson D. E., Lu J., Chen H., Werner S., Williams L. T. The human fibroblast growth factor receptor genes: a common structural arrangement underlies the mechanisms for generating receptor forms that differ in their third immunoglobulin domain. Mol Cell Biol. 1991 Sep;11(9):4627–4634. doi: 10.1128/mcb.11.9.4627. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kumar V., Trick M. Expression of the S-locus receptor kinase multigene family in Brassica oleracea. Plant J. 1994 Dec;6(6):807–813. doi: 10.1046/j.1365-313x.1994.6060807.x. [DOI] [PubMed] [Google Scholar]
- Mueller P. R., Wold B. In vivo footprinting of a muscle specific enhancer by ligation mediated PCR. Science. 1989 Nov 10;246(4931):780–786. doi: 10.1126/science.2814500. [DOI] [PubMed] [Google Scholar]
- Ochman H., Gerber A. S., Hartl D. L. Genetic applications of an inverse polymerase chain reaction. Genetics. 1988 Nov;120(3):621–623. doi: 10.1093/genetics/120.3.621. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PANDEY K. K. Time of the S allele action. Nature. 1958 Apr 26;181(4617):1220–1221. doi: 10.1038/1811220a0. [DOI] [PubMed] [Google Scholar]
- Pearce G., Johnson S., Ryan C. A. Structure-activity of deleted and substituted systemin, an 18-amino acid polypeptide inducer of plant defensive genes. J Biol Chem. 1993 Jan 5;268(1):212–216. [PubMed] [Google Scholar]
- Petch L. A., Harris J., Raymond V. W., Blasband A., Lee D. C., Earp H. S. A truncated, secreted form of the epidermal growth factor receptor is encoded by an alternatively spliced transcript in normal rat tissue. Mol Cell Biol. 1990 Jun;10(6):2973–2982. doi: 10.1128/mcb.10.6.2973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scott R., Dagless E., Hodge R., Paul W., Soufleri I., Draper J. Patterns of gene expression in developing anthers of Brassica napus. Plant Mol Biol. 1991 Aug;17(2):195–207. doi: 10.1007/BF00039494. [DOI] [PubMed] [Google Scholar]
- Stein J. C., Dixit R., Nasrallah M. E., Nasrallah J. B. SRK, the stigma-specific S locus receptor kinase of Brassica, is targeted to the plasma membrane in transgenic tobacco. Plant Cell. 1996 Mar;8(3):429–445. doi: 10.1105/tpc.8.3.429. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stein J. C., Howlett B., Boyes D. C., Nasrallah M. E., Nasrallah J. B. Molecular cloning of a putative receptor protein kinase gene encoded at the self-incompatibility locus of Brassica oleracea. Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8816–8820. doi: 10.1073/pnas.88.19.8816. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stein J. C., Nasrallah J. B. A plant receptor-like gene, the S-locus receptor kinase of Brassica oleracea L., encodes a functional serine/threonine kinase. Plant Physiol. 1993 Mar;101(3):1103–1106. doi: 10.1104/pp.101.3.1103. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ullrich A., Schlessinger J. Signal transduction by receptors with tyrosine kinase activity. Cell. 1990 Apr 20;61(2):203–212. doi: 10.1016/0092-8674(90)90801-k. [DOI] [PubMed] [Google Scholar]
- van de Sande K., Pawlowski K., Czaja I., Wieneke U., Schell J., Schmidt J., Walden R., Matvienko M., Wellink J., van Kammen A. Modification of phytohormone response by a peptide encoded by ENOD40 of legumes and a nonlegume. Science. 1996 Jul 19;273(5273):370–373. doi: 10.1126/science.273.5273.370. [DOI] [PubMed] [Google Scholar]