Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1995 Nov;109(3):1025–1031. doi: 10.1104/pp.109.3.1025

Involvement of Free Calcium in Action of Cryptogein, a Proteinaceous Elicitor of Hypersensitive Reaction in Tobacco Cells.

E Tavernier 1, D Wendehenne 1, J P Blein 1, A Pugin 1
PMCID: PMC161405  PMID: 12228650

Abstract

Treatment of suspension-cultured tobacco (Nicotiana tabacum var Xanthi) cells with cryptogein, a proteinaceous elicitor from Phytophthora cryptogea, induced a great stimulation of Ca2+ influx within the first minutes. Ca2+ influx is essential for the initiation of cryptogein-induced responses, since ethyleneglycol-bis([beta]-amino-ethyl ether)-N,N[prime]-tetraacetic acid or La3+, which block Ca2+ entrance, suppress cryptogein-induced responses such as extracellular alkalinization, active oxygen species, and phytoalexin production. Moreover, once initiated, these responses require sustained Ca2+ influx within the 1st h. A Ca2+ ionophore (A23187) was able to trigger an extracellular alkalinization but not the formation of active oxygen species and phytoalexins, even in the presence of cryptogein. Staurosporine, a protein kinase inhibitor that was recently reported to suppress cryptogein-induced responses (M.-P. Viard, F. Martin, A. Pugin, P. Ricci, J.-P. Blein [1994] Plant Physiol 104: 1245-1249), inhibited Ca2+ influx induced by cryptogein in a dose-dependent manner. These results suggest that protein phosphorylation followed by Ca2+ influx might be involved in the initial steps of cryptogein signal transduction.

Full Text

The Full Text of this article is available as a PDF (705.3 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atkinson M. M., Keppler L. D., Orlandi E. W., Baker C. J., Mischke C. F. Involvement of plasma membrane calcium influx in bacterial induction of the k/h and hypersensitive responses in tobacco. Plant Physiol. 1990 Jan;92(1):215–221. doi: 10.1104/pp.92.1.215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bach M., Schnitzler J. P., Seitz H. U. Elicitor-Induced Changes in Ca2+ Influx, K+ Efflux, and 4-Hydroxybenzoic Acid Synthesis in Protoplasts of Daucus carota L. Plant Physiol. 1993 Oct;103(2):407–412. doi: 10.1104/pp.103.2.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baker C. J., Orlandi E. W., Mock N. M. Harpin, An Elicitor of the Hypersensitive Response in Tobacco Caused by Erwinia amylovora, Elicits Active Oxygen Production in Suspension Cells. Plant Physiol. 1993 Aug;102(4):1341–1344. doi: 10.1104/pp.102.4.1341. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Blein J. P., Milat M. L., Ricci P. Responses of Cultured Tobacco Cells to Cryptogein, a Proteinaceous Elicitor from Phytophthora cryptogea: Possible Plasmalemma Involvement. Plant Physiol. 1991 Feb;95(2):486–491. doi: 10.1104/pp.95.2.486. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bush D. S. Regulation of Cytosolic Calcium in Plants. Plant Physiol. 1993 Sep;103(1):7–13. doi: 10.1104/pp.103.1.7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Clapham D. E. Calcium signaling. Cell. 1995 Jan 27;80(2):259–268. doi: 10.1016/0092-8674(95)90408-5. [DOI] [PubMed] [Google Scholar]
  7. Conrath U., Jeblick W., Kauss H. The protein kinase inhibitor, K-252a, decreases elicitor-induced Ca2+ uptake and K+ release, and increases coumarin synthesis in parsley cells. FEBS Lett. 1991 Feb 11;279(1):141–144. doi: 10.1016/0014-5793(91)80269-9. [DOI] [PubMed] [Google Scholar]
  8. Dietrich A., Mayer J. E., Hahlbrock K. Fungal elicitor triggers rapid, transient, and specific protein phosphorylation in parsley cell suspension cultures. J Biol Chem. 1990 Apr 15;265(11):6360–6368. [PubMed] [Google Scholar]
  9. Felix G., Grosskopf D. G., Regenass M., Boller T. Rapid changes of protein phosphorylation are involved in transduction of the elicitor signal in plant cells. Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8831–8834. doi: 10.1073/pnas.88.19.8831. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Felix G., Regenass M., Spanu P., Boller T. The protein phosphatase inhibitor calyculin A mimics elicitor action in plant cells and induces rapid hyperphosphorylation of specific proteins as revealed by pulse labeling with [33P]phosphate. Proc Natl Acad Sci U S A. 1994 Feb 1;91(3):952–956. doi: 10.1073/pnas.91.3.952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Johansson F., Sommarin M., Larsson C. Fusicoccin Activates the Plasma Membrane H+-ATPase by a Mechanism Involving the C-Terminal Inhibitory Domain. Plant Cell. 1993 Mar;5(3):321–327. doi: 10.1105/tpc.5.3.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nürnberger T., Nennstiel D., Jabs T., Sacks W. R., Hahlbrock K., Scheel D. High affinity binding of a fungal oligopeptide elicitor to parsley plasma membranes triggers multiple defense responses. Cell. 1994 Aug 12;78(3):449–460. doi: 10.1016/0092-8674(94)90423-5. [DOI] [PubMed] [Google Scholar]
  13. Ricci P., Bonnet P., Huet J. C., Sallantin M., Beauvais-Cante F., Bruneteau M., Billard V., Michel G., Pernollet J. C. Structure and activity of proteins from pathogenic fungi Phytophthora eliciting necrosis and acquired resistance in tobacco. Eur J Biochem. 1989 Aug 15;183(3):555–563. doi: 10.1111/j.1432-1033.1989.tb21084.x. [DOI] [PubMed] [Google Scholar]
  14. Schaller G. E., Harmon A. C., Sussman M. R. Characterization of a calcium- and lipid-dependent protein kinase associated with the plasma membrane of oat. Biochemistry. 1992 Feb 18;31(6):1721–1727. doi: 10.1021/bi00121a020. [DOI] [PubMed] [Google Scholar]
  15. Schumaker K. S., Gizinski M. J. Cytokinin stimulates dihydropyridine-sensitive calcium uptake in moss protoplasts. Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):10937–10941. doi: 10.1073/pnas.90.23.10937. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Thomine S., Zimmerman S., Van Duijn B., Barbier-Brygoo H., Guern J. Calcium channel antagonists induce direct inhibition of the outward rectifying potassium channel in tobacco protoplasts. FEBS Lett. 1994 Feb 28;340(1-2):45–50. doi: 10.1016/0014-5793(94)80170-3. [DOI] [PubMed] [Google Scholar]
  17. Trewavas A., Gilroy S. Signal transduction in plant cells. Trends Genet. 1991 Nov-Dec;7(11-12):356–361. doi: 10.1016/0168-9525(91)90255-o. [DOI] [PubMed] [Google Scholar]
  18. Vera-Estrella R., Barkla B. J., Higgins V. J., Blumwald E. Plant Defense Response to Fungal Pathogens (Activation of Host-Plasma Membrane H+-ATPase by Elicitor-Induced Enzyme Dephosphorylation). Plant Physiol. 1994 Jan;104(1):209–215. doi: 10.1104/pp.104.1.209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Viard M. P., Martin F., Pugin A., Ricci P., Blein J. P. Protein Phosphorylation Is Induced in Tobacco Cells by the Elicitor Cryptogein. Plant Physiol. 1994 Apr;104(4):1245–1249. doi: 10.1104/pp.104.4.1245. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Zook M. N., Rush J. S., Kuć J. A. A role for ca in the elicitation of rishitin and lubimin accumulation in potato tuber tissue. Plant Physiol. 1987 Jun;84(2):520–525. doi: 10.1104/pp.84.2.520. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES