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Purification of RK(His)6 from mammalian cells. (I) Chromatography on heparin–sepharose of RK(His)6 as in Materials and Methods. (II) Chromatography on cobalt–sepharose. Fractions 38–44 from I were pooled and chromatographed on a cobalt–sepharose column (Materials and Methods). (Inset) SDS/PAGE followed by Coomassie blue detection of fractions 32–44. L, preloading sample; M, molecular weight marker.
