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. 1995 Jan;39(1):260–263. doi: 10.1128/aac.39.1.260

Cloning, sequencing, and site-directed mutagenesis of beta-lactamase gene from Streptomyces fradiae Y59.

S Kurai 1, H Urabe 1, H Ogawara 1
PMCID: PMC162522  PMID: 7535038

Abstract

The beta-lactamase gene from Streptomyces fradiae Y59 was cloned and sequenced. To determine which amino acid residues are critical in binding activity to blue dextran, chimera beta-lactamases were constructed and their binding abilities were determined. The results suggested that blue dextran binding may depend more on overall conformation of about two-thirds of the beta-lactamase molecule from the N terminus than on the primary structure.

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Selected References

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