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. 1995 Feb;39(2):369–373. doi: 10.1128/aac.39.2.369

Prevalence of erm gene classes in erythromycin-resistant Staphylococcus aureus strains isolated between 1959 and 1988.

H Westh 1, D M Hougaard 1, J Vuust 1, V T Rosdahl 1
PMCID: PMC162545  PMID: 7726500

Abstract

The epidemiology of the two common erythromycin resistance methylase (erm) genes ermA and ermC was analyzed by Southern blotting in 428 erythromycin-resistant Staphylococcus aureus strains isolated from blood between 1959 and 1988 in Denmark. ermA and/or ermC was present in 98% of the erythromycin-resistant strains tested. ermA was found only as a chromosomal insert and was solely responsible for erythromycin resistance in these strains until about 1971. ermA was the only erm gene found in 337 strains and was a single insert in 61% of these strains, two inserts were seen in 37%, and three inserts were found in 2%. Thirteen different ermA EcoRI restriction fragment length polymorphisms were identified. ermA was not found in strains of phage type patterns group II and type 95, which are very common today. ermC was found on a plasmid in 77 strains. ermC was first seen in 1971 and spread rapidly in the S. aureus population, with a 5- to 10-fold increase every 5 years, and in 1984 to 1988, it was responsible for erythromycin resistance in 72% of the strains. The predominant plasmid carrying ermC was 2.5 kb, while four plasmids were smaller and three were larger. ermC has been found in all phage type patterns. Eight strains contained combinations of ermA and ermC, and no erm gene was detected in six strains.

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Selected References

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  1. CHABBERT Y. Antagonisme in vitro entre l'érythromycine et la spiramycine. Ann Inst Pasteur (Paris) 1956 Jun;90(6):787–790. [PubMed] [Google Scholar]
  2. Duval J. Evolution and epidemiology of MLS resistance. J Antimicrob Chemother. 1985 Jul;16 (Suppl A):137–149. doi: 10.1093/jac/16.suppl_a.137. [DOI] [PubMed] [Google Scholar]
  3. Eady E. A., Ross J. I., Tipper J. L., Walters C. E., Cove J. H., Noble W. C. Distribution of genes encoding erythromycin ribosomal methylases and an erythromycin efflux pump in epidemiologically distinct groups of staphylococci. J Antimicrob Chemother. 1993 Feb;31(2):211–217. doi: 10.1093/jac/31.2.211. [DOI] [PubMed] [Google Scholar]
  4. Horinouchi S., Byeon W. H., Weisblum B. A complex attenuator regulates inducible resistance to macrolides, lincosamides, and streptogramin type B antibiotics in Streptococcus sanguis. J Bacteriol. 1983 Jun;154(3):1252–1262. doi: 10.1128/jb.154.3.1252-1262.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Iordănescu S. Three distinct plasmids originating in the same Staphylococcus aureus strain. Arch Roum Pathol Exp Microbiol. 1976 Jan-Jun;35(1-2):111–118. [PubMed] [Google Scholar]
  6. Jenssen W. D., Thakker-Varia S., Dubin D. T., Weinstein M. P. Prevalence of macrolides-lincosamides-streptogramin B resistance and erm gene classes among clinical strains of staphylococci and streptococci. Antimicrob Agents Chemother. 1987 Jun;31(6):883–888. doi: 10.1128/aac.31.6.883. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kreiswirth B., Kornblum J., Arbeit R. D., Eisner W., Maslow J. N., McGeer A., Low D. E., Novick R. P. Evidence for a clonal origin of methicillin resistance in Staphylococcus aureus. Science. 1993 Jan 8;259(5092):227–230. doi: 10.1126/science.8093647. [DOI] [PubMed] [Google Scholar]
  8. Krolewski J. J., Murphy E., Novick R. P., Rush M. G. Site-specificity of the chromosomal insertion of Staphylococcus aureus transposon Tn554. J Mol Biol. 1981 Oct 15;152(1):19–33. doi: 10.1016/0022-2836(81)90093-0. [DOI] [PubMed] [Google Scholar]
  9. Lacey R. W. Antibiotic resistance in Staphylococcus aureus and streptococci. Br Med Bull. 1984 Jan;40(1):77–83. doi: 10.1093/oxfordjournals.bmb.a071951. [DOI] [PubMed] [Google Scholar]
  10. Lai C. J., Weisblum B. Altered methylation of ribosomal RNA in an erythromycin-resistant strain of Staphylococcus aureus. Proc Natl Acad Sci U S A. 1971 Apr;68(4):856–860. doi: 10.1073/pnas.68.4.856. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lampson B. C., Parisi J. T. Nucleotide sequence of the constitutive macrolide-lincosamide-streptogramin B resistance plasmid pNE131 from Staphylococcus epidermidis and homologies with Staphylococcus aureus plasmids pE194 and pSN2. J Bacteriol. 1986 Sep;167(3):888–892. doi: 10.1128/jb.167.3.888-892.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Larsson L. I., Hougaard D. M. Detection of gastrin and its messenger RNA in Zollinger-Ellison tumors by non-radioactive in situ hybridization and immunocytochemistry. Histochemistry. 1992;97(2):105–110. doi: 10.1007/BF00267299. [DOI] [PubMed] [Google Scholar]
  13. MITSUHASHI S., MORIMURA M., KONO K., OSHIMA H. ELIMINATION OF DRUG RESISTANCE OF STAPHYLOCOCCUS AUREUS BY TREATMENT WITH ACRIFLAVINE. J Bacteriol. 1963 Jul;86:162–164. doi: 10.1128/jb.86.1.162-164.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mekalanos J. J. Duplication and amplification of toxin genes in Vibrio cholerae. Cell. 1983 Nov;35(1):253–263. doi: 10.1016/0092-8674(83)90228-3. [DOI] [PubMed] [Google Scholar]
  15. Murphy E., Huwyler L., de Freire Bastos M. do C. Transposon Tn554: complete nucleotide sequence and isolation of transposition-defective and antibiotic-sensitive mutants. EMBO J. 1985 Dec 1;4(12):3357–3365. doi: 10.1002/j.1460-2075.1985.tb04089.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Murphy E., Reinheimer E., Huwyler L. Mutational analysis of att554, the target of the site-specific transposon Tn554. Plasmid. 1991 Jul;26(1):20–29. doi: 10.1016/0147-619x(91)90033-s. [DOI] [PubMed] [Google Scholar]
  17. Novick R. P., Murphy E., Gryczan T. J., Baron E., Edelman I. Penicillinase plasmids of Staphylococcus aureus: restriction-deletion maps. Plasmid. 1979 Jan;2(1):109–129. doi: 10.1016/0147-619x(79)90010-6. [DOI] [PubMed] [Google Scholar]
  18. Novick R. P., Murphy E. MLS-resistance determinants in Staphylococcus aureus and their molecular evolution. J Antimicrob Chemother. 1985 Jul;16 (Suppl A):101–110. doi: 10.1093/jac/16.suppl_a.101. [DOI] [PubMed] [Google Scholar]
  19. Parisi J. T., Robbins J., Lampson B. C., Hecht D. W. Characterization of a macrolide, lincosamide, and streptogramin resistance plasmid in Staphylococcus epidermidis. J Bacteriol. 1981 Nov;148(2):559–564. doi: 10.1128/jb.148.2.559-564.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rosdahl V. T. Localisation of the penicillinase gene in naturally occurring Staphylococcus aureus strains. Acta Pathol Microbiol Immunol Scand B. 1985 Dec;93(6):383–388. doi: 10.1111/j.1699-0463.1985.tb02906.x. [DOI] [PubMed] [Google Scholar]
  21. THOMSEN V. F. Correlation of the plate-dilution method to the agar diffusion method (disc and tablet methods) with a special view to the importance of pre-diffusion. Acta Pathol Microbiol Scand. 1962;54:107–120. doi: 10.1111/j.1699-0463.1962.tb01228.x. [DOI] [PubMed] [Google Scholar]
  22. THOMSEN V. F. THE RELATION BETWEEN INOCULUM AND ZONE SIZE IN SENSITIVITY TESTS BY THE AGAR DIFFUSION METHOD: WITH A SPECIAL VIEW TO THE IMPORTANCE OF PREDIFFUSION. Acta Pathol Microbiol Scand. 1964;61:303–316. doi: 10.1111/apm.1964.61.2.303. [DOI] [PubMed] [Google Scholar]
  23. Thakker-Varia S., Jenssen W. D., Moon-McDermott L., Weinstein M. P., Dubin D. T. Molecular epidemiology of macrolides-lincosamides-streptogramin B resistance in Staphylococcus aureus and coagulase-negative staphylococci. Antimicrob Agents Chemother. 1987 May;31(5):735–743. doi: 10.1128/aac.31.5.735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Thakker-Varia S., Ranzini A. C., Dubin D. T. Ribosomal RNA methylation in Staphylococcus aureus and Escherichia coli: effect of the "MLS" (erythromycin resistance) methylase. Plasmid. 1985 Sep;14(2):152–161. doi: 10.1016/0147-619x(85)90075-7. [DOI] [PubMed] [Google Scholar]
  25. Tillotson L. E., Jenssen W. D., Moon-McDermott L., Dubin D. T. Characterization of a novel insertion of the macrolides-lincosamides-streptogramin B resistance transposon Tn554 in methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis. Antimicrob Agents Chemother. 1989 Apr;33(4):541–550. doi: 10.1128/aac.33.4.541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Weisblum B., Demohn V. Erythromycin-inducible resistance in Staphylococcus aureus: survey of antibiotic classes involved. J Bacteriol. 1969 May;98(2):447–452. doi: 10.1128/jb.98.2.447-452.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Westh H., Jarløv J. O., Kjersem H., Rosdahl V. T. The disappearance of multiresistant Staphylococcus aureus in Denmark: changes in strains of the 83A complex between 1969 and 1989. Clin Infect Dis. 1992 Jun;14(6):1186–1194. doi: 10.1093/clinids/14.6.1186. [DOI] [PubMed] [Google Scholar]
  28. Westh H., Knudsen A. M., Gottschau A., Rosdahl V. T. Evolution of Staphylococcus aureus resistance to erythromycin in Denmark, 1959 to 1988: comparison with erythromycin-susceptible strains. J Hosp Infect. 1991 May;18(1):23–34. doi: 10.1016/0195-6701(91)90090-u. [DOI] [PubMed] [Google Scholar]
  29. Westh H., Knudsen A. M., Gottschau A., Rosdahl V. T. Evolution of Staphylococcus aureus resistance to erythromycin in Denmark, 1959 to 1988: correlations between characteristics of erythromycin-resistant bacteraemia strains. J Hosp Infect. 1991 May;18(1):35–43. doi: 10.1016/0195-6701(91)90091-l. [DOI] [PubMed] [Google Scholar]

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