Abstract
A new extended-spectrum beta-lactamase was detected in a lactose-positive Salmonella enterica subsp. enterica strain that caused a nosocomial outbreak involving eight patients in a pediatric cardiology unit. This strain showed high levels of resistance to ceftazidime and aztreonam and relatively low levels of resistance to cefotaxime and ceftriaxone. Resistance was associated with a conjugative plasmid of 59 kb, which encoded a new beta-lactamase with an isoelectric point of 5.9 that strongly hydrolyzed ceftazidime and to a much lesser extent hydrolyzed cefotaxime. The enzyme activity was inhibited by clavulanate. The corresponding bla gene was cloned and sequenced. The deduced amino acid sequence showed three significant amino acid replacements with respect to the TEM-1 sequence: Arg-164-->His, Glu-240-->Lys, and Thr-265-->Met. This combination is unique among extended-spectrum beta-lactamases and served to characterize the new enzyme, TEM-27.
Full Text
The Full Text of this article is available as a PDF (184.4 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Archambaud M., Gerbaud G., Labau E., Marty N., Courvalin P. Possible in-vivo transfer of beta-lactamase TEM-3 from Klebsiella pneumoniae to Salmonella kedougou. J Antimicrob Chemother. 1991 Apr;27(4):427–436. doi: 10.1093/jac/27.4.427. [DOI] [PubMed] [Google Scholar]
- Bauernfeind A., Hörl G. Novel R-factor borne beta-lactamase of Escherichia coli confering resistance to cephalosporins. Infection. 1987 Jul-Aug;15(4):257–259. doi: 10.1007/BF01644127. [DOI] [PubMed] [Google Scholar]
- Ben Redjeb S., Ben Yaghlane H., Boujnah A., Philippon A., Labia R. Synergy between clavulanic acid and newer beta-lactams on nine clinical isolates of Klebsiella pneumoniae, Escherichia coli and Salmonella typhimurium resistant to third-generation cephalosporins. J Antimicrob Chemother. 1988 Feb;21(2):263–266. doi: 10.1093/jac/21.2.263. [DOI] [PubMed] [Google Scholar]
- Bush K., Sykes R. B. Methodology for the study of beta-lactamases. Antimicrob Agents Chemother. 1986 Jul;30(1):6–10. doi: 10.1128/aac.30.1.6. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chanal C., Poupart M. C., Sirot D., Labia R., Sirot J., Cluzel R. Nucleotide sequences of CAZ-2, CAZ-6, and CAZ-7 beta-lactamase genes. Antimicrob Agents Chemother. 1992 Sep;36(9):1817–1820. doi: 10.1128/aac.36.9.1817. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chen S. T., Clowes R. C. Variations between the nucleotide sequences of Tn1, Tn2, and Tn3 and expression of beta-lactamase in Pseudomonas aeruginosa and Escherichia coli. J Bacteriol. 1987 Feb;169(2):913–916. doi: 10.1128/jb.169.2.913-916.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fernandez-Rodriguez A., Canton R., Perez-Diaz J. C., Martinez-Beltran J., Picazo J. J., Baquero F. Aminoglycoside-modifying enzymes in clinical isolates harboring extended-spectrum beta-lactamases. Antimicrob Agents Chemother. 1992 Nov;36(11):2536–2538. doi: 10.1128/aac.36.11.2536. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goussard S., Sougakoff W., Mabilat C., Bauernfeind A., Courvalin P. An IS1-like element is responsible for high-level synthesis of extended-spectrum beta-lactamase TEM-6 in Enterobacteriaceae. J Gen Microbiol. 1991 Dec;137(12):2681–2687. doi: 10.1099/00221287-137-12-2681. [DOI] [PubMed] [Google Scholar]
- Gutmann L., Kitzis M. D., Billot-Klein D., Goldstein F., Tran Van Nhieu G., Lu T., Carlet J., Collatz E., Williamson R. Plasmid-mediated beta-lactamase (TEM-7) involved in resistance to ceftazidime and aztreonam. Rev Infect Dis. 1988 Jul-Aug;10(4):860–866. doi: 10.1093/clinids/10.4.860. [DOI] [PubMed] [Google Scholar]
- Hammami A., Arlet G., Ben Redjeb S., Grimont F., Ben Hassen A., Rekik A., Philippon A. Nosocomial outbreak of acute gastroenteritis in a neonatal intensive care unit in Tunisia caused by multiply drug resistant Salmonella wien producing SHV-2 beta-lactamase. Eur J Clin Microbiol Infect Dis. 1991 Aug;10(8):641–646. doi: 10.1007/BF01975816. [DOI] [PubMed] [Google Scholar]
- Jacoby G. A., Medeiros A. A. More extended-spectrum beta-lactamases. Antimicrob Agents Chemother. 1991 Sep;35(9):1697–1704. doi: 10.1128/aac.35.9.1697. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Knothe H., Shah P., Krcmery V., Antal M., Mitsuhashi S. Transferable resistance to cefotaxime, cefoxitin, cefamandole and cefuroxime in clinical isolates of Klebsiella pneumoniae and Serratia marcescens. Infection. 1983 Nov-Dec;11(6):315–317. doi: 10.1007/BF01641355. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Mabilat C., Courvalin P. Development of "oligotyping" for characterization and molecular epidemiology of TEM beta-lactamases in members of the family Enterobacteriaceae. Antimicrob Agents Chemother. 1990 Nov;34(11):2210–2216. doi: 10.1128/aac.34.11.2210. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mabilat C., Goussard S., Sougakoff W., Spencer R. C., Courvalin P. Direct sequencing of the amplified structural gene and promoter for the extended-broad-spectrum beta-lactamase TEM-9 (RHH-1) of Klebsiella pneumoniae. Plasmid. 1990 Jan;23(1):27–34. doi: 10.1016/0147-619x(90)90041-a. [DOI] [PubMed] [Google Scholar]
- Mariotte S., Nordmann P., Nicolas M. H. Extended-spectrum beta-lactamase in Proteus mirabilis. J Antimicrob Chemother. 1994 May;33(5):925–935. doi: 10.1093/jac/33.5.925. [DOI] [PubMed] [Google Scholar]
- Paul G. C., Gerbaud G., Bure A., Philippon A. M., Pangon B., Courvalin P. TEM-4, a new plasmid-mediated beta-lactamase that hydrolyzes broad-spectrum cephalosporins in a clinical isolate of Escherichia coli. Antimicrob Agents Chemother. 1989 Nov;33(11):1958–1963. doi: 10.1128/aac.33.11.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Poupart M. C., Chanal C., Sirot D., Labia R., Sirot J. Identification of CTX-2, a novel cefotaximase from a Salmonella mbandaka isolate. Antimicrob Agents Chemother. 1991 Jul;35(7):1498–1500. doi: 10.1128/aac.35.7.1498. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rasmussen B. A., Bradford P. A., Quinn J. P., Wiener J., Weinstein R. A., Bush K. Genetically diverse ceftazidime-resistant isolates from a single center: biochemical and genetic characterization of TEM-10 beta-lactamases encoded by different nucleotide sequences. Antimicrob Agents Chemother. 1993 Sep;37(9):1989–1992. doi: 10.1128/aac.37.9.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sancar A., Hack A. M., Rupp W. D. Simple method for identification of plasmid-coded proteins. J Bacteriol. 1979 Jan;137(1):692–693. doi: 10.1128/jb.137.1.692-693.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sougakoff W., Petit A., Goussard S., Sirot D., Bure A., Courvalin P. Characterization of the plasmid genes blaT-4 and blaT-5 which encode the broad-spectrum beta-lactamases TEM-4 and TEM-5 in enterobacteriaceae. Gene. 1989 May 30;78(2):339–348. doi: 10.1016/0378-1119(89)90236-9. [DOI] [PubMed] [Google Scholar]
- Sowek J. A., Singer S. B., Ohringer S., Malley M. F., Dougherty T. J., Gougoutas J. Z., Bush K. Substitution of lysine at position 104 or 240 of TEM-1pTZ18R beta-lactamase enhances the effect of serine-164 substitution on hydrolysis or affinity for cephalosporins and the monobactam aztreonam. Biochemistry. 1991 Apr 2;30(13):3179–3188. doi: 10.1021/bi00227a004. [DOI] [PubMed] [Google Scholar]
- Spratt B. G., Hedge P. J., te Heesen S., Edelman A., Broome-Smith J. K. Kanamycin-resistant vectors that are analogues of plasmids pUC8, pUC9, pEMBL8 and pEMBL9. Gene. 1986;41(2-3):337–342. doi: 10.1016/0378-1119(86)90117-4. [DOI] [PubMed] [Google Scholar]
- Sutcliffe J. G. Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3737–3741. doi: 10.1073/pnas.75.8.3737. [DOI] [PMC free article] [PubMed] [Google Scholar]