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. 1995 Jun;39(6):1300–1305. doi: 10.1128/aac.39.6.1300

Interactions of biapenem with active-site serine and metallo-beta-lactamases.

A Felici 1, M Perilli 1, B Segatore 1, N Franceschini 1, D Setacci 1, A Oratore 1, S Stefani 1, M Galleni 1, G Amicosante 1
PMCID: PMC162731  PMID: 7574520

Abstract

Biapenem, formerly LJC 10,627 or L-627, a carbapenem antibiotic, was studied in its interactions with 12 beta-lactamases belonging to the four molecular classes proposed by R. P. Ambler (Philos. Trans. R. Soc. Lond. Biol. Sci. 289:321-331, 1980). Kinetic parameters were determined. Biapenem was readily inactivated by metallo-beta-lactamases but behaved as a transient inhibitor of the active-site serine enzymes tested, although with different acylation efficiency values. Class A and class D beta-lactamases were unable to confer in vitro resistance toward this carbapenem antibiotic. Surprisingly, the same situation was found in the case of class B enzymes from Aeromonas hydrophila AE036 and Bacillus cereus 5/B/6 when expressed in Escherichia coli strains.

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Selected References

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