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. 1983 Feb;58(2):115–119. doi: 10.1136/adc.58.2.115

Upper intestinal bacterial flora during transpyloric feeding.

H D Dellagrammaticas, B I Duerden, R D Milner
PMCID: PMC1628092  PMID: 6402990

Abstract

Samples from the pharynx, stomach, duodenum or jejunum, and faeces were collected on 7 days between 1st and 28th day from neonates weighing less than 1.5 kg at birth who were fed by transpyloric tube. These were cultured on selective and non-selective media, and the results were expressed in a semi-quantitative manner. The number of bacterial species and the density of their growth increased with the patient's age; this was particularly noticeable with Gram-negative bacteria and the ratio of Gram-negative to Gram-positive organisms increased steadily in specimens from all sites with increasing age. The upper small intestine was more heavily colonised than the stomach early in life and the microflora present was predominantly faecal in nature. The species isolated from all sites were mainly aerobes or facultative anaerobes; strict anaerobes did not form a significant proportion of the microflora in these infants. Necrotising enterocolitis developed only after heavy jejunal colonisation with Gram-negative bacilli.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERSON C. M., LANGFORD R. F. Bacterial content of small intestine of children in health, in coeliac disease, and in fibrocystic disease of pancreas. Br Med J. 1958 Apr 5;1(5074):803–806. doi: 10.1136/bmj.1.5074.803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson C. M., Challacombe D. N., Richardson J. M. The bacterial flora of the upper gastrointestinal tract in children both in health and disease. Soc Appl Bacteriol Symp Ser. 1974;3(0):197–203. [PubMed] [Google Scholar]
  3. Boros S. J., Reynolds J. W. Duodenal perforation: a complication of neonatal nasojejunal feeding. J Pediatr. 1974 Jul;85(1):107–108. doi: 10.1016/s0022-3476(74)80301-x. [DOI] [PubMed] [Google Scholar]
  4. Boxall J., Gendle N. Intensive care of the newborn--6.--Measuring and passing a duodenal/jejunal feeding tube. Nurs Times. 1979 Aug 23;75(34):1459–1460. [PubMed] [Google Scholar]
  5. Challacombe D. N., Richardson J. M., Anderson C. M. Bacterial microflora of the upper gastrointestinal tract in infants without diarrhoea. Arch Dis Child. 1974 Apr;49(4):264–269. doi: 10.1136/adc.49.4.264. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Drasar B. S. Some factors associated with geographical variations in the intestinal microflora. Soc Appl Bacteriol Symp Ser. 1974;3(0):187–196. [PubMed] [Google Scholar]
  7. Gorbach S. L., Plaut A. G., Nahas L., Weinstein L., Spanknebel G., Levitan R. Studies of intestinal microflora. II. Microorganisms of the small intestine and their relations to oral and fecal flora. Gastroenterology. 1967 Dec;53(6):856–867. [PubMed] [Google Scholar]
  8. Gustafson B. U., Kjellman B. Use of transpyloric tube feeding with nonpasteurized human milk. J Pediatr. 1981 Aug;99(2):300–302. doi: 10.1016/s0022-3476(81)80483-0. [DOI] [PubMed] [Google Scholar]
  9. Heird W. C. Nasojejunal feeding: a commentary. J Pediatr. 1974 Jul;85(1):111–112. doi: 10.1016/s0022-3476(74)80303-3. [DOI] [PubMed] [Google Scholar]
  10. Kalser M. H., Cohen R., Arteaga I., Yawn E., Mayoral L., Hoffert W. R., Frazier D. Normal viral and bacterial flora of the human small and large intestine. N Engl J Med. 1966 Mar 3;274(9):500–contd. doi: 10.1056/NEJM196603032740907. [DOI] [PubMed] [Google Scholar]
  11. Milner R. D., Minoli I., Moro G., Rubecz I., Whitfield M. F., Assan R. Growth and metabolic and hormonal profiles during transpyloric and nasogastric feeding in preterm infants. Acta Paediatr Scand. 1981 Jan;70(1):9–13. doi: 10.1111/j.1651-2227.1981.tb07165.x. [DOI] [PubMed] [Google Scholar]
  12. Minoli I., Moro G., Ovadia M. F. Nasoduodenal feeding in high risk newborns. Acta Paediatr Scand. 1978 Mar;67(2):161–168. doi: 10.1111/j.1651-2227.1978.tb16297.x. [DOI] [PubMed] [Google Scholar]
  13. Rhea J. W., Kilby J. O. A nasojejunal tube for infant feeding. Pediatrics. 1970 Jul;46(1):36–40. [PubMed] [Google Scholar]
  14. Rotimi V. O., Duerden B. I. The development of the bacterial flora in normal neonates. J Med Microbiol. 1981 Feb;14(1):51–62. doi: 10.1099/00222615-14-1-51. [DOI] [PubMed] [Google Scholar]
  15. Roy R. N., Pollnitz R. B., Hamilton J. R., Chance G. W. Impaired assimilation of nasojejunal feeds in healthy low-birth-weight newborn infants. J Pediatr. 1977 Mar;90(3):431–434. doi: 10.1016/s0022-3476(77)80710-5. [DOI] [PubMed] [Google Scholar]
  16. Vazquez C., Arroyos A., Vallis I Soler A. Necrotising entercolitis. Increased incidence in infants receiving nasoduodenal feeding. Arch Dis Child. 1980 Oct;55(10):826–826. doi: 10.1136/adc.55.10.826. [DOI] [PMC free article] [PubMed] [Google Scholar]

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