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. 1995 Sep;39(9):2167–2168. doi: 10.1128/aac.39.9.2167

Culture microtitration: a sensitive method for quantifying Leishmania infantum in tissues of infected mice.

P A Buffet 1, A Sulahian 1, Y J Garin 1, N Nassar 1, F Derouin 1
PMCID: PMC162906  PMID: 8540741

Abstract

We developed a microtitration method to determine the parasite burdens in homogenized organs of mice infected with Leishmania infantum. This method proved more sensitive than direct enumeration of amastigotes in stained organs, was appropriate for describing the kinetics of infection, and can be considered for physiopathological or pharmaceutical experimental studies.

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Selected References

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