Skip to main content
PMC home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1995 Oct;39(10):2359–2363. doi: 10.1128/aac.39.10.2359

Nucleotide sequence and characterization of erythromycin resistance determinant that encodes macrolide 2'-phosphotransferase I in Escherichia coli.

N Noguchi 1, A Emura 1, H Matsuyama 1, K O'Hara 1, M Sasatsu 1, M Kono 1
PMCID: PMC162946  PMID: 8619599

Abstract

The DNA fragment (3.3 kb) containing the erythromycin resistance determinant was cloned from Escherichia coli Tf481A and sequenced. Deletion and complementation analyses indicated that the expression of high-level resistance to erythromycin requires two genes, mphA and mrx, which encode macrolide 2'-phosphotransferase I and an unidentified hydrophobic protein, respectively.

Full Text

The Full Text of this article is available as a PDF (348.5 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chang A. C., Cohen S. N. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol. 1978 Jun;134(3):1141–1156. doi: 10.1128/jb.134.3.1141-1156.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gold L., Pribnow D., Schneider T., Shinedling S., Singer B. S., Stormo G. Translational initiation in prokaryotes. Annu Rev Microbiol. 1981;35:365–403. doi: 10.1146/annurev.mi.35.100181.002053. [DOI] [PubMed] [Google Scholar]
  3. Grinsted J., Bennett P. M., Richmond M. H. A restriction enzyme map of R-plasmid RP1. Plasmid. 1977 Nov;1(1):34–37. doi: 10.1016/0147-619x(77)90006-3. [DOI] [PubMed] [Google Scholar]
  4. Grinsted J., Saunders J. R., Ingram L. C., Sykes R. B., Richmond M. H. Properties of a R factor which originated in Pseudomonas aeruginosa 1822. J Bacteriol. 1972 May;110(2):529–537. doi: 10.1128/jb.110.2.529-537.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Henikoff S. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene. 1984 Jun;28(3):351–359. doi: 10.1016/0378-1119(84)90153-7. [DOI] [PubMed] [Google Scholar]
  6. Iida S., Meyer J., Linder P., Goto N., Nakaya R., Reif H. J., Arber W. The kanamycin resistance transposon Tn2680 derived from the R plasmid Rts1 and carried by phage P1Km has flanking 0.8-kb-long direct repeats. Plasmid. 1982 Sep;8(2):187–198. doi: 10.1016/0147-619x(82)90056-7. [DOI] [PubMed] [Google Scholar]
  7. Kunkel T. A., Roberts J. D., Zakour R. A. Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 1987;154:367–382. doi: 10.1016/0076-6879(87)54085-x. [DOI] [PubMed] [Google Scholar]
  8. Kyte J., Doolittle R. F. A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982 May 5;157(1):105–132. doi: 10.1016/0022-2836(82)90515-0. [DOI] [PubMed] [Google Scholar]
  9. Labigne-Roussel A., Briaux-Gerbaud S., Courvalin P. Tn1525, a kanamycin R determinant flanked by two direct copies of IS15. Mol Gen Genet. 1983;189(1):90–101. doi: 10.1007/BF00326060. [DOI] [PubMed] [Google Scholar]
  10. O'Hara K., Kanda T., Kono M. Structure of a phosphorylated derivative of oleandomycin, obtained by reaction of oleandomycin with an extract of an erythromycin-resistant strain of Escherichia coli. J Antibiot (Tokyo) 1988 Jun;41(6):823–827. doi: 10.7164/antibiotics.41.823. [DOI] [PubMed] [Google Scholar]
  11. Ouellette M., Gerbaud G., Lambert T., Courvalin P. Acquisition by a Campylobacter-like strain of aphA-1, a kanamycin resistance determinant from members of the family Enterobacteriaceae. Antimicrob Agents Chemother. 1987 Jul;31(7):1021–1026. doi: 10.1128/aac.31.7.1021. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Selzer G., Som T., Itoh T., Tomizawa J. The origin of replication of plasmid p15A and comparative studies on the nucleotide sequences around the origin of related plasmids. Cell. 1983 Jan;32(1):119–129. doi: 10.1016/0092-8674(83)90502-0. [DOI] [PubMed] [Google Scholar]
  14. Uemori T., Ishino Y., Fujita K., Asada K., Kato I. Cloning of the DNA polymerase gene of Bacillus caldotenax and characterization of the gene product. J Biochem. 1993 Mar;113(3):401–410. doi: 10.1093/oxfordjournals.jbchem.a124058. [DOI] [PubMed] [Google Scholar]
  15. Wrighton C. J., Strike P. A pathway for the evolution of the plasmid NTP16 involving the novel kanamycin resistance transposon Tn4352. Plasmid. 1987 Jan;17(1):37–45. doi: 10.1016/0147-619x(87)90006-0. [DOI] [PubMed] [Google Scholar]
  16. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES