Abstract
Dictyostelium discoideum, a soil eukaryote, is highly resistant to DNA-damaging agents; repair mutants are more susceptible. Susceptibility to bleomycin, produced by Streptomyces verticillus, is greater for mutants which are susceptible to other agents than for resistant strains. The high potential for DNA repair may result from the need to cope with chemicals produced by other soil microorganisms.
Full Text
The Full Text of this article is available as a PDF (175.7 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Blazquez J., Martinez J. L., Baquero F. Bleomycin increases amikacin and streptomycin resistance in Escherichia coli harboring transposon Tn5. Antimicrob Agents Chemother. 1993 Sep;37(9):1982–1985. doi: 10.1128/aac.37.9.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Blot M., Heitman J., Arber W. Tn5-mediated bleomycin resistance in Escherichia coli requires the expression of host genes. Mol Microbiol. 1993 Jun;8(6):1017–1024. doi: 10.1111/j.1365-2958.1993.tb01646.x. [DOI] [PubMed] [Google Scholar]
- Bronner C. E., Welker D. L., Deering R. A. Mutations affecting sensitivity of the cellular slime mold Dictyostelium discoideum to DNA-damaging agents. Mutat Res. 1992 Sep;274(3):187–200. doi: 10.1016/0921-8777(92)90065-b. [DOI] [PubMed] [Google Scholar]
- Chang A. C., Hall R. M., Williams K. L. Bleomycin resistance as a selectable marker for transformation of the eukaryote, Dictyostelium discoideum. Gene. 1991 Oct 30;107(1):165–170. doi: 10.1016/0378-1119(91)90312-y. [DOI] [PubMed] [Google Scholar]
- Giaccia A. J., Shieh J., Cholon A., Brown J. M. Biochemical evidence for two different mechanisms for bleomycin-induced cell killing. Mutat Res. 1991 Jun;263(2):69–75. doi: 10.1016/0165-7992(91)90062-9. [DOI] [PubMed] [Google Scholar]
- Harris A. L., Hochhauser D. Mechanisms of multidrug resistance in cancer treatment. Acta Oncol. 1992;31(2):205–213. doi: 10.3109/02841869209088904. [DOI] [PubMed] [Google Scholar]
- Howard P. K., Ahern K. G., Firtel R. A. Establishment of a transient expression system for Dictyostelium discoideum. Nucleic Acids Res. 1988 Mar 25;16(6):2613–2623. doi: 10.1093/nar/16.6.2613. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Povirk L. F., Han Y. H., Steighner R. J. Structure of bleomycin-induced DNA double-strand breaks: predominance of blunt ends and single-base 5' extensions. Biochemistry. 1989 Jul 11;28(14):5808–5814. doi: 10.1021/bi00440a016. [DOI] [PubMed] [Google Scholar]
- Povirk L. F., Houlgrave C. W. Effect of apurinic/apyrimidinic endonucleases and polyamines on DNA treated with bleomycin and neocarzinostatin: specific formation and cleavage of closely opposed lesions in complementary strands. Biochemistry. 1988 May 17;27(10):3850–3857. doi: 10.1021/bi00410a049. [DOI] [PubMed] [Google Scholar]
- Povirk L. F., Wübter W., Köhnlein W., Hutchinson F. DNA double-strand breaks and alkali-labile bonds produced by bleomycin. Nucleic Acids Res. 1977 Oct;4(10):3573–3580. doi: 10.1093/nar/4.10.3573. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Simon S. M., Schindler M. Cell biological mechanisms of multidrug resistance in tumors. Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3497–3504. doi: 10.1073/pnas.91.9.3497. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tounekti O., Pron G., Belehradek J., Jr, Mir L. M. Bleomycin, an apoptosis-mimetic drug that induces two types of cell death depending on the number of molecules internalized. Cancer Res. 1993 Nov 15;53(22):5462–5469. [PubMed] [Google Scholar]
- Welker D. L., Deering R. A. Interactions between radiation-sensitive mutations in double-mutant haploids of Dictyostelium discoideum. Mol Gen Genet. 1979 Jan 2;167(3):265–270. doi: 10.1007/BF00267418. [DOI] [PubMed] [Google Scholar]