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Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1996 Feb;40(2):477–480. doi: 10.1128/aac.40.2.477

Mutations in 23S rRNA are associated with clarithromycin resistance in Helicobacter pylori.

J Versalovic 1, D Shortridge 1, K Kibler 1, M V Griffy 1, J Beyer 1, R K Flamm 1, S K Tanaka 1, D Y Graham 1, M F Go 1
PMCID: PMC163139  PMID: 8834903

Abstract

Twelve clarithromycin-resistant Helicobacter pylori isolates (100% of resistant isolates examined) from seven different patients each contained an A-->G transition mutation within a conserved loop of 23S rRNA. A-->G transition mutations at positions cognate with Escherichia coli 23S rRNA positions 2058 and 2059 were identified. Clarithromycin-susceptible H. pylori isolates from 14 different patients displayed no polymorphisms in a conserved loop within domain V of 23S rRNA. The study is the first to report mutations in H. pylori associated with resistance to an antimicrobial agent used in established peptic ulcer treatment regimens.

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Selected References

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  1. Bayerdörffer E., Neubauer A., Rudolph B., Thiede C., Lehn N., Eidt S., Stolte M. Regression of primary gastric lymphoma of mucosa-associated lymphoid tissue type after cure of Helicobacter pylori infection. MALT Lymphoma Study Group. Lancet. 1995 Jun 24;345(8965):1591–1594. doi: 10.1016/s0140-6736(95)90113-2. [DOI] [PubMed] [Google Scholar]
  2. Bukanov N. O., Berg D. E. Ordered cosmid library and high-resolution physical-genetic map of Helicobacter pylori strain NCTC11638. Mol Microbiol. 1994 Feb;11(3):509–523. doi: 10.1111/j.1365-2958.1994.tb00332.x. [DOI] [PubMed] [Google Scholar]
  3. Cseplö A., Etzold T., Schell J., Schreier P. H. Point mutations in the 23 S rRNA genes of four lincomycin resistant Nicotiana plumbaginifolia mutants could provide new selectable markers for chloroplast transformation. Mol Gen Genet. 1988 Oct;214(2):295–299. doi: 10.1007/BF00337724. [DOI] [PubMed] [Google Scholar]
  4. Ettayebi M., Prasad S. M., Morgan E. A. Chloramphenicol-erythromycin resistance mutations in a 23S rRNA gene of Escherichia coli. J Bacteriol. 1985 May;162(2):551–557. doi: 10.1128/jb.162.2.551-557.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Go M. F., Chan K. Y., Versalovic J., Koeuth T., Graham D. Y., Lupski J. R. Cluster analysis of Helicobacter pylori genomic DNA fingerprints suggests gastroduodenal disease-specific associations. Scand J Gastroenterol. 1995 Jul;30(7):640–646. doi: 10.3109/00365529509096306. [DOI] [PubMed] [Google Scholar]
  6. Goldman R. C., Zakula D., Flamm R., Beyer J., Capobianco J. Tight binding of clarithromycin, its 14-(R)-hydroxy metabolite, and erythromycin to Helicobacter pylori ribosomes. Antimicrob Agents Chemother. 1994 Jul;38(7):1496–1500. doi: 10.1128/aac.38.7.1496. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Graham D. Y., Lew G. M., Klein P. D., Evans D. G., Evans D. J., Jr, Saeed Z. A., Malaty H. M. Effect of treatment of Helicobacter pylori infection on the long-term recurrence of gastric or duodenal ulcer. A randomized, controlled study. Ann Intern Med. 1992 May 1;116(9):705–708. doi: 10.7326/0003-4819-116-9-705. [DOI] [PubMed] [Google Scholar]
  8. Hardy D. J., Hanson C. W., Hensey D. M., Beyer J. M., Fernandes P. B. Susceptibility of Campylobacter pylori to macrolides and fluoroquinolones. J Antimicrob Chemother. 1988 Nov;22(5):631–636. doi: 10.1093/jac/22.5.631. [DOI] [PubMed] [Google Scholar]
  9. Labenz J., Börsch G. Highly significant change of the clinical course of relapsing and complicated peptic ulcer disease after cure of Helicobacter pylori infection. Am J Gastroenterol. 1994 Oct;89(10):1785–1788. [PubMed] [Google Scholar]
  10. Logan R. P., Gummett P. A., Schaufelberger H. D., Greaves R. R., Mendelson G. M., Walker M. M., Thomas P. H., Baron J. H., Misiewicz J. J. Eradication of Helicobacter pylori with clarithromycin and omeprazole. Gut. 1994 Mar;35(3):323–326. doi: 10.1136/gut.35.3.323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Marshall B. J. Helicobacter pylori. Am J Gastroenterol. 1994 Aug;89(8 Suppl):S116–S128. [PubMed] [Google Scholar]
  12. Meier A., Kirschner P., Springer B., Steingrube V. A., Brown B. A., Wallace R. J., Jr, Böttger E. C. Identification of mutations in 23S rRNA gene of clarithromycin-resistant Mycobacterium intracellulare. Antimicrob Agents Chemother. 1994 Feb;38(2):381–384. doi: 10.1128/aac.38.2.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Menninger J. R. Functional consequences of binding macrolides to ribosomes. J Antimicrob Chemother. 1985 Jul;16 (Suppl A):23–34. doi: 10.1093/jac/16.suppl_a.23. [DOI] [PubMed] [Google Scholar]
  14. Moazed D., Noller H. F. Chloramphenicol, erythromycin, carbomycin and vernamycin B protect overlapping sites in the peptidyl transferase region of 23S ribosomal RNA. Biochimie. 1987 Aug;69(8):879–884. doi: 10.1016/0300-9084(87)90215-x. [DOI] [PubMed] [Google Scholar]
  15. Skinner R., Cundliffe E., Schmidt F. J. Site of action of a ribosomal RNA methylase responsible for resistance to erythromycin and other antibiotics. J Biol Chem. 1983 Oct 25;258(20):12702–12706. [PubMed] [Google Scholar]
  16. Sor F., Fukuhara H. Identification of two erythromycin resistance mutations in the mitochondrial gene coding for the large ribosomal RNA in yeast. Nucleic Acids Res. 1982 Nov 11;10(21):6571–6577. doi: 10.1093/nar/10.21.6571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Stolte M., Eidt S. Healing gastric MALT lymphomas by eradicating H pylori? Lancet. 1993 Sep 4;342(8871):568–568. doi: 10.1016/0140-6736(93)91404-a. [DOI] [PubMed] [Google Scholar]
  18. Sung J. J., Chung S. C., Ling T. K., Yung M. Y., Leung V. K., Ng E. K., Li M. K., Cheng A. F., Li A. K. Antibacterial treatment of gastric ulcers associated with Helicobacter pylori. N Engl J Med. 1995 Jan 19;332(3):139–142. doi: 10.1056/NEJM199501193320302. [DOI] [PubMed] [Google Scholar]
  19. Vester B., Garrett R. A. A plasmid-coded and site-directed mutation in Escherichia coli 23S RNA that confers resistance to erythromycin: implications for the mechanism of action of erythromycin. Biochimie. 1987 Aug;69(8):891–900. doi: 10.1016/0300-9084(87)90217-3. [DOI] [PubMed] [Google Scholar]
  20. Weisblum B. Erythromycin resistance by ribosome modification. Antimicrob Agents Chemother. 1995 Mar;39(3):577–585. doi: 10.1128/AAC.39.3.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wotherspoon A. C., Doglioni C., Diss T. C., Pan L., Moschini A., de Boni M., Isaacson P. G. Regression of primary low-grade B-cell gastric lymphoma of mucosa-associated lymphoid tissue type after eradication of Helicobacter pylori. Lancet. 1993 Sep 4;342(8871):575–577. doi: 10.1016/0140-6736(93)91409-f. [DOI] [PubMed] [Google Scholar]

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