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. 1996 Apr;40(4):930–933. doi: 10.1128/aac.40.4.930

Comparison of antimicrobial susceptibilities of Corynebacterium species by broth microdilution and disk diffusion methods.

K Weiss 1, M Laverdière 1, R Rivest 1
PMCID: PMC163233  PMID: 8849254

Abstract

Corynebacterium species are increasingly being implicated in foreign-body infections and in immunocompromised-host infections. However, there are no specific recommendations on the method or the criteria to use in order to determine the in vitro activities of the antibiotics commonly used to treat Corynebacterium infections. The first aim of our study was to compare the susceptibilities of various species of Corynebacterium to vancomycin, erythromycin, and penicillin by using a broth microdilution method and a disk diffusion method. Second, the activity of penicillin against our isolates was assessed by using the interpretative criteria recommended by the National Committee for Clinical Laboratory Standards for the determination of the susceptibility of streptococci and Listeria monocytogenes to penicillin. Overall, 100% of the isolates were susceptible to vancomycin, while considerable variations in the activities of erythromycin and penicillin were noted for the different species tested, including the non-Corynebacterium jeikeium species. A good correlation in the susceptibilities of vancomycin and erythromycin between the disk diffusion and the microdilution methods was observed. However, a 5% rate of major or very major errors was detected with the Listeria criteria, while a high rate of minor errors (18%) was noted when the streptococcus criteria were used. Our findings indicate considerable variations in the activities of erythromycin and penicillin against the various species of Corynebacterium. Because of the absence of definite recommendations, important discrepancies were observed between the methods and the interpretations of the penicillin activity.

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Selected References

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  1. Berger S. A., Gorea A., Stadler J., Dan M., Zilberman M. Recurrent breast abscesses caused by Corynebacterium minutissimum. J Clin Microbiol. 1984 Dec;20(6):1219–1220. doi: 10.1128/jcm.20.6.1219-1220.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cavendish J., Cole J. B., Ohl C. A. Polymicrobial central venous catheter sepsis involving a multiantibiotic-resistant strain of Corynebacterium minutissimum. Clin Infect Dis. 1994 Jul;19(1):204–205. doi: 10.1093/clinids/19.1.204. [DOI] [PubMed] [Google Scholar]
  3. Coyle M. B., Leonard R. B., Nowowiejski D. J., Malekniazi A., Finn D. J. Evidence of multiple taxa within commercially available reference strains of Corynebacterium xerosis. J Clin Microbiol. 1993 Jul;31(7):1788–1793. doi: 10.1128/jcm.31.7.1788-1793.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Coyle M. B., Lipsky B. A. Coryneform bacteria in infectious diseases: clinical and laboratory aspects. Clin Microbiol Rev. 1990 Jul;3(3):227–246. doi: 10.1128/cmr.3.3.227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. De Briel D., Couderc F., Riegel P., Jehl F., Minck R. High-performance liquid chromatography of corynomycolic acids as a tool in identification of Corynebacterium species and related organisms. J Clin Microbiol. 1992 Jun;30(6):1407–1417. doi: 10.1128/jcm.30.6.1407-1417.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Funke G., Falsen E., Barreau C. Primary identification of Microbacterium spp. encountered in clinical specimens as CDC coryneform group A-4 and A-5 bacteria. J Clin Microbiol. 1995 Jan;33(1):188–192. doi: 10.1128/jcm.33.1.188-192.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Funke G., Stubbs S., Pfyffer G. E., Marchiani M., Collins M. D. Characteristics of CDC group 3 and group 5 coryneform bacteria isolated from clinical specimens and assignment to the genus Dermabacter. J Clin Microbiol. 1994 May;32(5):1223–1228. doi: 10.1128/jcm.32.5.1223-1228.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. García-Rodriguez J. A., García Sánchez J. E., Muñoz Bellido J. L., Nebreda Mayoral T., García Sánchez E., García García I. In vitro activity of 79 antimicrobial agents against Corynebacterium group D2. Antimicrob Agents Chemother. 1991 Oct;35(10):2140–2143. doi: 10.1128/aac.35.10.2140. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Martínez-Martínez L., Ortega M. C., Suárez A. I. Comparison of E-test with broth microdilution and disk diffusion for susceptibility testing of coryneform bacteria. J Clin Microbiol. 1995 May;33(5):1318–1321. doi: 10.1128/jcm.33.5.1318-1321.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Morris A. J., Henderson G. K., Bremner D. A., Collins J. F. Relapsing peritonitis in a patient undergoing continuous ambulatory peritoneal dialysis due to Corynebacterium aquaticum. J Infect. 1986 Sep;13(2):151–156. doi: 10.1016/s0163-4453(86)92925-7. [DOI] [PubMed] [Google Scholar]
  11. Philippon A., Bimet F. In vitro susceptibility of Corynebacterium group D2 and Corynebacterium jeikeium to twelve antibiotics. Eur J Clin Microbiol Infect Dis. 1990 Dec;9(12):892–895. doi: 10.1007/BF01967505. [DOI] [PubMed] [Google Scholar]
  12. Riley P. S., Hollis D. G., Utter G. B., Weaver R. E., Baker C. N. Characterization and identification of 95 diphtheroid (group JK) cultures isolated from clinical specimens. J Clin Microbiol. 1979 Mar;9(3):418–424. doi: 10.1128/jcm.9.3.418-424.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Soriano F., Zapardiel J., Nieto E. Antimicrobial susceptibilities of Corynebacterium species and other non-spore-forming gram-positive bacilli to 18 antimicrobial agents. Antimicrob Agents Chemother. 1995 Jan;39(1):208–214. doi: 10.1128/aac.39.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Watkins D. A., Chahine A., Creger R. J., Jacobs M. R., Lazarus H. M. Corynebacterium striatum: a diphtheroid with pathogenic potential. Clin Infect Dis. 1993 Jul;17(1):21–25. doi: 10.1093/clinids/17.1.21. [DOI] [PubMed] [Google Scholar]
  15. Williams D. Y., Selepak S. T., Gill V. J. Identification of clinical isolates of nondiphtherial Corynebacterium species and their antibiotic susceptibility patterns. Diagn Microbiol Infect Dis. 1993 Jul;17(1):23–28. doi: 10.1016/0732-8893(93)90065-f. [DOI] [PubMed] [Google Scholar]
  16. Zapardiel J., Nieto E., Gegúndez M. I., Gadea I., Soriano F. Problems in minimum inhibitory concentration determinations in coryneform organisms. Comparison of an agar dilution and the Etest. Diagn Microbiol Infect Dis. 1994 Jul;19(3):171–173. doi: 10.1016/0732-8893(94)90062-0. [DOI] [PubMed] [Google Scholar]

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