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. 1996 May;40(5):1263–1265. doi: 10.1128/aac.40.5.1263

Plasmid-borne high-level resistance to gentamicin in Enterococcus hirae, Enterococcus avium, and Enterococcus raffinosus.

M Straut 1, G de Cespédès 1, T Horaud 1
PMCID: PMC163304  PMID: 8723479

Abstract

Enterococcus hirae, E. avium, and E. raffinosus isolated in Romania, Tunisia, and Portugal harbored plasmids pICC8, pIP1700, and pIP1701, respectively, encoding resistance to high levels of gentamicin (Gmr). The Gmr marker was carried on pIP1700 by a Tn4001-like element and on pICC8 and pIP1701 by Tn4001-truncated structures. pICC8 carried, in addition to Gmr, chloramphenicol, erythromycin, and tetracycline-minocycline (TetM) resistance determinants. The gene tetM of pICC8 was carried on a Tn916-like element.

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Selected References

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  1. Bentorcha F., Clermont D., de Cespédès G., Horaud T. Natural occurrence of structures in oral streptococci and enterococci with DNA homology to Tn916. Antimicrob Agents Chemother. 1992 Jan;36(1):59–63. doi: 10.1128/aac.36.1.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bentorcha F., De Cespédès G., Horaud T. Tetracycline resistance heterogeneity in Enterococcus faecium. Antimicrob Agents Chemother. 1991 May;35(5):808–812. doi: 10.1128/aac.35.5.808. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burdett V., Inamine J., Rajagopalan S. Heterogeneity of tetracycline resistance determinants in Streptococcus. J Bacteriol. 1982 Mar;149(3):995–1004. doi: 10.1128/jb.149.3.995-1004.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Christie P. J., Korman R. Z., Zahler S. A., Adsit J. C., Dunny G. M. Two conjugation systems associated with Streptococcus faecalis plasmid pCF10: identification of a conjugative transposon that transfers between S. faecalis and Bacillus subtilis. J Bacteriol. 1987 Jun;169(6):2529–2536. doi: 10.1128/jb.169.6.2529-2536.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dyke K. G., Aubert S., el Solh N. Multiple copies of IS256 in staphylococci. Plasmid. 1992 Nov;28(3):235–246. doi: 10.1016/0147-619x(92)90055-f. [DOI] [PubMed] [Google Scholar]
  6. Eliopoulos G. M., Wennersten C., Zighelboim-Daum S., Reiszner E., Goldmann D., Moellering R. C., Jr High-level resistance to gentamicin in clinical isolates of Streptococcus (Enterococcus) faecium. Antimicrob Agents Chemother. 1988 Oct;32(10):1528–1532. doi: 10.1128/aac.32.10.1528. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Facklam R. R., Collins M. D. Identification of Enterococcus species isolated from human infections by a conventional test scheme. J Clin Microbiol. 1989 Apr;27(4):731–734. doi: 10.1128/jcm.27.4.731-734.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fletcher H. M., Daneo-Moore L. A truncated Tn916-like element in a clinical isolate of Enterococcus faecium. Plasmid. 1992 Mar;27(2):155–160. doi: 10.1016/0147-619x(92)90015-3. [DOI] [PubMed] [Google Scholar]
  9. Gawron-Burke C., Clewell D. B. Regeneration of insertionally inactivated streptococcal DNA fragments after excision of transposon Tn916 in Escherichia coli: strategy for targeting and cloning of genes from gram-positive bacteria. J Bacteriol. 1984 Jul;159(1):214–221. doi: 10.1128/jb.159.1.214-221.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hodel-Christian S. L., Murray B. E. Characterization of the gentamicin resistance transposon Tn5281 from Enterococcus faecalis and comparison to staphylococcal transposons Tn4001 and Tn4031. Antimicrob Agents Chemother. 1991 Jun;35(6):1147–1152. doi: 10.1128/aac.35.6.1147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Horaud T., Delbos F., Pepper K. Does a tetracycline resistance determinant of class N exist? Antimicrob Agents Chemother. 1990 Jul;34(7):1447–1449. doi: 10.1128/aac.34.7.1447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Horodniceanu T., Bougueleret L., El-Solh N., Bieth G., Delbos F. High-level, plasmid-borne resistance to gentamicin in Streptococcus faecalis subsp. zymogenes. Antimicrob Agents Chemother. 1979 Nov;16(5):686–689. doi: 10.1128/aac.16.5.686. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Horodniceanu T., Bougueleret L., El-Solh N., Bouanchaud D. H., Chabbert Y. A. Conjugative R plasmids in Streptococcus agalactiae (group B). Plasmid. 1979 Apr;2(2):197–206. doi: 10.1016/0147-619x(79)90038-6. [DOI] [PubMed] [Google Scholar]
  14. Jacob A. E., Hobbs S. J. Conjugal transfer of plasmid-borne multiple antibiotic resistance in Streptococcus faecalis var. zymogenes. J Bacteriol. 1974 Feb;117(2):360–372. doi: 10.1128/jb.117.2.360-372.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Le Bouguénec C., de Cespédès G., Horaud T. Molecular analysis of a composite chromosomal conjugative element (Tn3701) of Streptococcus pyogenes. J Bacteriol. 1988 Sep;170(9):3930–3936. doi: 10.1128/jb.170.9.3930-3936.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lyon B. R., May J. W., Skurray R. A. Tn4001: a gentamicin and kanamycin resistance transposon in Staphylococcus aureus. Mol Gen Genet. 1984;193(3):554–556. doi: 10.1007/BF00382099. [DOI] [PubMed] [Google Scholar]
  17. Martin P., Trieu-Cuot P., Courvalin P. Nucleotide sequence of the tetM tetracycline resistance determinant of the streptococcal conjugative shuttle transposon Tn1545. Nucleic Acids Res. 1986 Sep 11;14(17):7047–7058. doi: 10.1093/nar/14.17.7047. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mederski-Samoraj B. D., Murray B. E. High-level resistance to gentamicin in clinical isolates of enterococci. J Infect Dis. 1983 Apr;147(4):751–757. doi: 10.1093/infdis/147.4.751. [DOI] [PubMed] [Google Scholar]
  19. Pepper K., Horaud T., Le Bouguénec C., de Cespédès G. Location of antibiotic resistance markers in clinical isolates of Enterococcus faecalis with similar antibiotypes. Antimicrob Agents Chemother. 1987 Sep;31(9):1394–1402. doi: 10.1128/aac.31.9.1394. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sahm D. F., Boonlayangoor S., Schulz J. E. Detection of high-level aminoglycoside resistance in enterococci other than Enterococcus faecalis. J Clin Microbiol. 1991 Nov;29(11):2595–2598. doi: 10.1128/jcm.29.11.2595-2598.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sahm D. F., Gilmore M. S. Transferability and genetic relatedness of high-level gentamicin resistance among enterococci. Antimicrob Agents Chemother. 1994 May;38(5):1194–1196. doi: 10.1128/aac.38.5.1194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Shaw J. H., Clewell D. B. Complete nucleotide sequence of macrolide-lincosamide-streptogramin B-resistance transposon Tn917 in Streptococcus faecalis. J Bacteriol. 1985 Nov;164(2):782–796. doi: 10.1128/jb.164.2.782-796.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Trieu-Cuot P., de Cespédès G., Bentorcha F., Delbos F., Gaspar E., Horaud T. Study of heterogeneity of chloramphenicol acetyltransferase (CAT) genes in streptococci and enterococci by polymerase chain reaction: characterization of a new CAT determinant. Antimicrob Agents Chemother. 1993 Dec;37(12):2593–2598. doi: 10.1128/aac.37.12.2593. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Watanakunakorn C. Rapid increase in the prevalence of high-level aminoglycoside resistance among enterococci isolated from blood cultures during 1989-1991. J Antimicrob Chemother. 1992 Sep;30(3):289–293. doi: 10.1093/jac/30.3.289. [DOI] [PubMed] [Google Scholar]

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