Abstract
Reporter strains of Mycobacterium tuberculosis and Mycobacterium bovis BCG endogenously expressing firefly luciferase were used in bioluminescence assays to evaluate the activities of isoniazid and rifampin against mycobacteria sequestered in human macrophages. This methodology allowed the efficacy of antibiotics against intracellular mycobacteria to be assessed without the labor-intensive procedures and protracted incubation requirements associated with conventional CFU determinations.
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- Clini V., Grassi C. The action of new antituberculous drugs on intracellular tubercle bacilli. Antibiot Chemother. 1970;16:20–26. doi: 10.1159/000386799. [DOI] [PubMed] [Google Scholar]
- Cooksey R. C., Crawford J. T., Jacobs W. R., Jr, Shinnick T. M. A rapid method for screening antimicrobial agents for activities against a strain of Mycobacterium tuberculosis expressing firefly luciferase. Antimicrob Agents Chemother. 1993 Jun;37(6):1348–1352. doi: 10.1128/aac.37.6.1348. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dhillon J., Mitchison D. A. Activity in vitro of rifabutin, FCE 22807, rifapentine, and rifampin against Mycobacterium microti and M. tuberculosis and their penetration into mouse peritoneal macrophages. Am Rev Respir Dis. 1992 Jan;145(1):212–214. doi: 10.1164/ajrccm/145.1.212. [DOI] [PubMed] [Google Scholar]
- Jacobs W. R., Jr, Barletta R. G., Udani R., Chan J., Kalkut G., Sosne G., Kieser T., Sarkis G. J., Hatfull G. F., Bloom B. R. Rapid assessment of drug susceptibilities of Mycobacterium tuberculosis by means of luciferase reporter phages. Science. 1993 May 7;260(5109):819–822. doi: 10.1126/science.8484123. [DOI] [PubMed] [Google Scholar]
- Johnson J. D., Hand W. L., Francis J. B., King-Thompson N., Corwin R. W. Antibiotic uptake by alveolar macrophages. J Lab Clin Med. 1980 Mar;95(3):429–439. [PubMed] [Google Scholar]
- Mor N., Heifets L. MICs and MBCs of clarithromycin against Mycobacterium avium within human macrophages. Antimicrob Agents Chemother. 1993 Jan;37(1):111–114. doi: 10.1128/aac.37.1.111. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mulazimoglu L., Drenning S. D., Muder R. R. Vancomycin-gentamicin synergism revisited: effect of gentamicin susceptibility of methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 1996 Jun;40(6):1534–1535. doi: 10.1128/aac.40.6.1534. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Onyeji C. O., Nightingale C. H., Nicolau D. P., Quintiliani R. Efficacies of liposome-encapsulated clarithromycin and ofloxacin against Mycobacterium avium-M. intracellulare complex in human macrophages. Antimicrob Agents Chemother. 1994 Mar;38(3):523–527. doi: 10.1128/aac.38.3.523. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Prokesch R. C., Hand W. L. Antibiotic entry into human polymorphonuclear leukocytes. Antimicrob Agents Chemother. 1982 Mar;21(3):373–380. doi: 10.1128/aac.21.3.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sbarbaro J. A., Iseman M. D., Crowle A. J. The combined effect of rifampin and pyrazinamide within the human macrophage. Am Rev Respir Dis. 1992 Dec;146(6):1448–1451. doi: 10.1164/ajrccm/146.6.1448. [DOI] [PubMed] [Google Scholar]