Skip to main content
PMC home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1996 Oct;40(10):2410–2412. doi: 10.1128/aac.40.10.2410

Differential release of smooth-type lipopolysaccharide from Pseudomonas aeruginosa treated with carbapenem antibiotics and its relation to production of tumor necrosis factor alpha and nitric oxide.

T Yokochi 1, A Kusumi 1, N Kido 1, Y Kato 1, T Sugiyama 1, N Koide 1, G Z Jiang 1, K Narita 1, K Takahashi 1
PMCID: PMC163543  PMID: 8891153

Abstract

Endotoxin release from Pseudomonas aeruginosa treated with cell wall-active carbapenem antibiotics and its effect on the production of tumor necrosis factor alpha and nitric oxide were examined. Treatment of bacteria with imipenem induced much lower levels of endotoxin release than treatment with meropenem. The endotoxin released was demonstrated to be of the smooth type and O-specific polysaccharide-rich. The exposure of the filtrates of P. aeruginosa treated with imipenem to physiologically relevant cells caused low-level production of tumor necrosis factor alpha and nitric oxide, while similar treatment with meropenem induced high levels of production.

Full Text

The Full Text of this article is available as a PDF (191.2 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albina J. E., Cui S., Mateo R. B., Reichner J. S. Nitric oxide-mediated apoptosis in murine peritoneal macrophages. J Immunol. 1993 Jun 1;150(11):5080–5085. [PubMed] [Google Scholar]
  2. Arditi M., Kabat W., Yogev R. Antibiotic-induced bacterial killing stimulates tumor necrosis factor-alpha release in whole blood. J Infect Dis. 1993 Jan;167(1):240–244. doi: 10.1093/infdis/167.1.240. [DOI] [PubMed] [Google Scholar]
  3. Crosby H. A., Bion J. F., Penn C. W., Elliott T. S. Antibiotic-induced release of endotoxin from bacteria in vitro. J Med Microbiol. 1994 Jan;40(1):23–30. doi: 10.1099/00222615-40-1-23. [DOI] [PubMed] [Google Scholar]
  4. Dofferhoff A. S., Esselink M. T., de Vries-Hospers H. G., van Zanten A., Bom V. J., Weits J., Vellenga E. The release of endotoxin from antibiotic-treated Escherichia coli and the production of tumour necrosis factor by human monocytes. J Antimicrob Chemother. 1993 Mar;31(3):373–384. doi: 10.1093/jac/31.3.373. [DOI] [PubMed] [Google Scholar]
  5. Dofferhoff A. S., Nijland J. H., de Vries-Hospers H. G., Mulder P. O., Weits J., Bom V. J. Effects of different types and combinations of antimicrobial agents on endotoxin release from gram-negative bacteria: an in-vitro and in-vivo study. Scand J Infect Dis. 1991;23(6):745–754. doi: 10.3109/00365549109024303. [DOI] [PubMed] [Google Scholar]
  6. Eng R. H., Smith S. M., Fan-Havard P., Ogbara T. Effect of antibiotics on endotoxin release from gram-negative bacteria. Diagn Microbiol Infect Dis. 1993 Mar-Apr;16(3):185–189. doi: 10.1016/0732-8893(93)90109-k. [DOI] [PubMed] [Google Scholar]
  7. Friedland I. R., Jafari H., Ehrett S., Rinderknecht S., Paris M., Coulthard M., Saxen H., Olsen K., McCracken G. H., Jr Comparison of endotoxin release by different antimicrobial agents and the effect on inflammation in experimental Escherichia coli meningitis. J Infect Dis. 1993 Sep;168(3):657–662. doi: 10.1093/infdis/168.3.657. [DOI] [PubMed] [Google Scholar]
  8. Green L. C., Wagner D. A., Glogowski J., Skipper P. L., Wishnok J. S., Tannenbaum S. R. Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids. Anal Biochem. 1982 Oct;126(1):131–138. doi: 10.1016/0003-2697(82)90118-x. [DOI] [PubMed] [Google Scholar]
  9. Hitchcock P. J., Brown T. M. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol. 1983 Apr;154(1):269–277. doi: 10.1128/jb.154.1.269-277.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hurley J. C. Antibiotic-induced release of endotoxin. A therapeutic paradox. Drug Saf. 1995 Mar;12(3):183–195. doi: 10.2165/00002018-199512030-00004. [DOI] [PubMed] [Google Scholar]
  11. Hurley J. C. Antibiotic-induced release of endotoxin: a reappraisal. Clin Infect Dis. 1992 Nov;15(5):840–854. doi: 10.1093/clind/15.5.840. [DOI] [PubMed] [Google Scholar]
  12. Hurley J. C., Louis W. J., Tosolini F. A., Carlin J. B. Antibiotic-induced release of endotoxin in chronically bacteriuric patients. Antimicrob Agents Chemother. 1991 Nov;35(11):2388–2394. doi: 10.1128/aac.35.11.2388. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jackson J. J., Kropp H. beta-Lactam antibiotic-induced release of free endotoxin: in vitro comparison of penicillin-binding protein (PBP) 2-specific imipenem and PBP 3-specific ceftazidime. J Infect Dis. 1992 Jun;165(6):1033–1041. doi: 10.1093/infdis/165.6.1033. [DOI] [PubMed] [Google Scholar]
  14. Mock C. N., Jurkovich G. J., Dries D. J., Maier R. V. Clinical significance of antibiotic endotoxin-releasing properties in trauma patients. Arch Surg. 1995 Nov;130(11):1234–1241. doi: 10.1001/archsurg.1995.01430110092017. [DOI] [PubMed] [Google Scholar]
  15. Prins J. M., Kuijper E. J., Mevissen M. L., Speelman P., van Deventer S. J. Release of tumor necrosis factor alpha and interleukin 6 during antibiotic killing of Escherichia coli in whole blood: influence of antibiotic class, antibiotic concentration, and presence of septic serum. Infect Immun. 1995 Jun;63(6):2236–2242. doi: 10.1128/iai.63.6.2236-2242.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Prins J. M., van Agtmael M. A., Kuijper E. J., van Deventer S. J., Speelman P. Antibiotic-induced endotoxin release in patients with gram-negative urosepsis: a double-blind study comparing imipenem and ceftazidime. J Infect Dis. 1995 Sep;172(3):886–891. doi: 10.1093/infdis/172.3.886. [DOI] [PubMed] [Google Scholar]
  17. Prins J. M., van Deventer S. J., Kuijper E. J., Speelman P. Clinical relevance of antibiotic-induced endotoxin release. Antimicrob Agents Chemother. 1994 Jun;38(6):1211–1218. doi: 10.1128/aac.38.6.1211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Simon D. M., Koenig G., Trenholme G. M. Differences in release of tumor necrosis factor from THP-1 cells stimulated by filtrates of antibiotic-killed Escherichia coli. J Infect Dis. 1991 Oct;164(4):800–802. doi: 10.1093/infdis/164.4.800. [DOI] [PubMed] [Google Scholar]
  19. Täuber M. G., Shibl A. M., Hackbarth C. J., Larrick J. W., Sande M. A. Antibiotic therapy, endotoxin concentration in cerebrospinal fluid, and brain edema in experimental Escherichia coli meningitis in rabbits. J Infect Dis. 1987 Sep;156(3):456–462. doi: 10.1093/infdis/156.3.456. [DOI] [PubMed] [Google Scholar]
  20. Van Den Berg C., de Neeling A. J., Schot C. S., Hustinx W. N., Wemer J., de Wildt D. J. Delayed antibiotic-induced lysis of Escherichia coli in vitro is correlated with enhancement of LPS release. Scand J Infect Dis. 1992;24(5):619–627. doi: 10.3109/00365549209054648. [DOI] [PubMed] [Google Scholar]
  21. Yokochi T., Inoue Y., Yokoo J., Kimura Y., Kato N. Retention of bacterial lipopolysaccharide at the site of subcutaneous injection. Infect Immun. 1989 Jun;57(6):1786–1791. doi: 10.1128/iai.57.6.1786-1791.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES