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. 1996 Nov;40(11):2550–2554. doi: 10.1128/aac.40.11.2550

Detection of genes regulating beta-lactamase production in Enterococcus faecalis and Staphylococcus aureus.

R Okamoto 1, T Okubo 1, M Inoue 1
PMCID: PMC163573  PMID: 8913462

Abstract

Four beta-lactamase-producing clinical isolates (WH245, WH257, CH570, and DEL) of Enterococcus faecalis were examined for the presence of the staphylococcal beta-lactamase regulatory genes (blaR1 and blaI) by PCR using six primer pairs. All isolates produced small amounts of beta-lactamase constitutively. In WH245, CH570, and DEL, the corresponding regions of the regulatory genes have lost sequences of various lengths. However, the regulatory genes in WH257 appeared to be the same as those in staphylococcal plasmid pI258. The beta-lactamase genes could be transferred to enterococcal and staphylococcal recipients from WH257 and DEL by conjugation or transformation with selection for gentamicin resistance. After transformation, the expression of beta-lactamase from DEL was still constitutive, whereas the gene from WH257 showed inducible expression in Staphylococcus aureus. The gene coding for inducible beta-lactamase production from pI258 showed constitutive expression in E. faecalis. These findings suggest that constitutive beta-lactamase production in E. faecalis is due not only to the absence of functional regulatory genes but to some other factor(s) as well.

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Selected References

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  1. Chow J. W., Perri M. B., Thal L. A., Zervos M. J. Mobilization of the penicillinase gene in Enterococcus faecalis. Antimicrob Agents Chemother. 1993 May;37(5):1187–1189. doi: 10.1128/aac.37.5.1187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Clewell D. B., Tomich P. K., Gawron-Burke M. C., Franke A. E., Yagi Y., An F. Y. Mapping of Streptococcus faecalis plasmids pAD1 and pAD2 and studies relating to transposition of Tn917. J Bacteriol. 1982 Dec;152(3):1220–1230. doi: 10.1128/jb.152.3.1220-1230.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Handwerger S., Perlman D. C., Altarac D., McAuliffe V. Concomitant high-level vancomycin and penicillin resistance in clinical isolates of enterococci. Clin Infect Dis. 1992 Mar;14(3):655–661. doi: 10.1093/clinids/14.3.655. [DOI] [PubMed] [Google Scholar]
  4. Hiraoka M., Okamoto R., Inoue M., Mitsuhashi S. Effects of beta-lactamases and omp mutation on susceptibility to beta-lactam antibiotics in Escherichia coli. Antimicrob Agents Chemother. 1989 Mar;33(3):382–386. doi: 10.1128/aac.33.3.382. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kreiswirth B. N., Löfdahl S., Betley M. J., O'Reilly M., Schlievert P. M., Bergdoll M. S., Novick R. P. The toxic shock syndrome exotoxin structural gene is not detectably transmitted by a prophage. Nature. 1983 Oct 20;305(5936):709–712. doi: 10.1038/305709a0. [DOI] [PubMed] [Google Scholar]
  6. Markowitz S. M., Wells V. D., Williams D. S., Stuart C. G., Coudron P. E., Wong E. S. Antimicrobial susceptibility and molecular epidemiology of beta-lactamase-producing, aminoglycoside-resistant isolates of Enterococcus faecalis. Antimicrob Agents Chemother. 1991 Jun;35(6):1075–1080. doi: 10.1128/aac.35.6.1075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Matsuhashi M., Song M. D., Ishino F., Wachi M., Doi M., Inoue M., Ubukata K., Yamashita N., Konno M. Molecular cloning of the gene of a penicillin-binding protein supposed to cause high resistance to beta-lactam antibiotics in Staphylococcus aureus. J Bacteriol. 1986 Sep;167(3):975–980. doi: 10.1128/jb.167.3.975-980.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mazzulli T., King S. M., Richardson S. E. Bacteremia due to beta-lactamase-producing Enterococcus faecalis with high-level resistance to gentamicin in a child with Wiskott-Aldrich syndrome. Clin Infect Dis. 1992 Mar;14(3):780–781. doi: 10.1093/clinids/14.3.780. [DOI] [PubMed] [Google Scholar]
  9. Murray B. E. Beta-lactamase-producing enterococci. Antimicrob Agents Chemother. 1992 Nov;36(11):2355–2359. doi: 10.1128/aac.36.11.2355. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Murray B. E., Church D. A., Wanger A., Zscheck K., Levison M. E., Ingerman M. J., Abrutyn E., Mederski-Samoraj B. Comparison of two beta-lactamase-producing strains of Streptococcus faecalis. Antimicrob Agents Chemother. 1986 Dec;30(6):861–864. doi: 10.1128/aac.30.6.861. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Murray B. E., Lopardo H. A., Rubeglio E. A., Frosolono M., Singh K. V. Intrahospital spread of a single gentamicin-resistant, beta-lactamase-producing strain of Enterococcus faecalis in Argentina. Antimicrob Agents Chemother. 1992 Jan;36(1):230–232. doi: 10.1128/aac.36.1.230. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Murray B. E., Mederski-Samaroj B. Transferable beta-lactamase. A new mechanism for in vitro penicillin resistance in Streptococcus faecalis. J Clin Invest. 1983 Sep;72(3):1168–1171. doi: 10.1172/JCI111042. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Murray B. E., Mederski-Samoraj B., Foster S. K., Brunton J. L., Harford P. In vitro studies of plasmid-mediated penicillinase from Streptococcus faecalis suggest a staphylococcal origin. J Clin Invest. 1986 Jan;77(1):289–293. doi: 10.1172/JCI112289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Murray B. E., Singh K. V., Markowitz S. M., Lopardo H. A., Patterson J. E., Zervos M. J., Rubeglio E., Eliopoulos G. M., Rice L. B., Goldstein F. W. Evidence for clonal spread of a single strain of beta-lactamase-producing Enterococcus (Streptococcus) faecalis to six hospitals in five states. J Infect Dis. 1991 Apr;163(4):780–785. doi: 10.1093/infdis/163.4.780. [DOI] [PubMed] [Google Scholar]
  15. Murray B. E. The life and times of the Enterococcus. Clin Microbiol Rev. 1990 Jan;3(1):46–65. doi: 10.1128/cmr.3.1.46. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Patterson J. E., Colodny S. M., Zervos M. J. Serious infection due to beta-lactamase-producing Streptococcus faecalis with high-level resistance to gentamicin. J Infect Dis. 1988 Nov;158(5):1144–1145. doi: 10.1093/infdis/158.5.1144. [DOI] [PubMed] [Google Scholar]
  17. Patterson J. E., Wanger A., Zscheck K. K., Zervos M. J., Murray B. E. Molecular epidemiology of beta-lactamase-producing enterococci. Antimicrob Agents Chemother. 1990 Feb;34(2):302–305. doi: 10.1128/aac.34.2.302. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rhinehart E., Smith N. E., Wennersten C., Gorss E., Freeman J., Eliopoulos G. M., Moellering R. C., Jr, Goldmann D. A. Rapid dissemination of beta-lactamase-producing, aminoglycoside-resistant Enterococcus faecalis among patients and staff on an infant-toddler surgical ward. N Engl J Med. 1990 Dec 27;323(26):1814–1818. doi: 10.1056/NEJM199012273232606. [DOI] [PubMed] [Google Scholar]
  19. Rice L. B., Carias L. L., Marshall S. H., Bonafede M. E. Sequences found on staphylococcal beta-lactamase plasmids integrated into the chromosome of Enterococcus faecalis CH116. Plasmid. 1996 Mar;35(2):81–90. doi: 10.1006/plas.1996.0010. [DOI] [PubMed] [Google Scholar]
  20. Rice L. B., Eliopoulos G. M., Wennersten C., Goldmann D., Jacoby G. A., Moellering R. C., Jr Chromosomally mediated beta-lactamase production and gentamicin resistance in Enterococcus faecalis. Antimicrob Agents Chemother. 1991 Feb;35(2):272–276. doi: 10.1128/aac.35.2.272. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rice L. B., Marshall S. H. Evidence of incorporation of the chromosomal beta-lactamase gene of Enterococcus faecalis CH19 into a transposon derived from staphylococci. Antimicrob Agents Chemother. 1992 Sep;36(9):1843–1846. doi: 10.1128/aac.36.9.1843. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Rice L. B., Marshall S. H. Insertions of IS256-like element flanking the chromosomal beta-lactamase gene of Enterococcus faecalis CX19. Antimicrob Agents Chemother. 1994 Apr;38(4):693–701. doi: 10.1128/aac.38.4.693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rowland S. J., Dyke K. G. Tn552, a novel transposable element from Staphylococcus aureus. Mol Microbiol. 1990 Jun;4(6):961–975. doi: 10.1111/j.1365-2958.1990.tb00669.x. [DOI] [PubMed] [Google Scholar]
  24. Shalita Z., Murphy E., Novick R. P. Penicillinase plasmids of Staphylococcus aureus: structural and evolutionary relationships. Plasmid. 1980 May;3(3):291–311. doi: 10.1016/0147-619x(80)90042-6. [DOI] [PubMed] [Google Scholar]
  25. Smith M. C., Murray B. E. Comparison of enterococcal and staphylococcal beta-lactamase-encoding fragments. Antimicrob Agents Chemother. 1992 Feb;36(2):273–276. doi: 10.1128/aac.36.2.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Smith M. C., Murray B. E. Sequence analysis of the beta-lactamase repressor from Staphylococcus aureus and hybridization studies with two beta-lactamase-producing isolates of Enterococcus faecalis. Antimicrob Agents Chemother. 1992 Oct;36(10):2265–2269. doi: 10.1128/aac.36.10.2265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Tomayko J. F., Murray B. E. Analysis of Enterococcus faecalis isolates from intercontinental sources by multilocus enzyme electrophoresis and pulsed-field gel electrophoresis. J Clin Microbiol. 1995 Nov;33(11):2903–2907. doi: 10.1128/jcm.33.11.2903-2907.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Tomayko J. F., Zscheck K. K., Singh K. V., Murray B. E. Comparison of the beta-lactamase gene cluster in clonally distinct strains of Enterococcus faecalis. Antimicrob Agents Chemother. 1996 May;40(5):1170–1174. doi: 10.1128/aac.40.5.1170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Waley S. G. A spectrophotometric assay of beta-lactamase action on penicillins. Biochem J. 1974 Jun;139(3):789–790. doi: 10.1042/bj1390789. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Wang P. Z., Projan S. J., Novick R. P. Nucleotide sequence of beta-lactamase regulatory genes from staphylococcal plasmid pI258. Nucleic Acids Res. 1991 Jul 25;19(14):4000–4000. doi: 10.1093/nar/19.14.4000. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Wells V. D., Wong E. S., Murray B. E., Coudron P. E., Williams D. S., Markowitz S. M. Infections due to beta-lactamase-producing, high-level gentamicin-resistant Enterococcus faecalis. Ann Intern Med. 1992 Feb 15;116(4):285–292. doi: 10.7326/0003-4819-116-4-285. [DOI] [PubMed] [Google Scholar]
  32. Yamagishi J., Kojima T., Oyamada Y., Fujimoto K., Hattori H., Nakamura S., Inoue M. Alterations in the DNA topoisomerase IV grlA gene responsible for quinolone resistance in Staphylococcus aureus. Antimicrob Agents Chemother. 1996 May;40(5):1157–1163. doi: 10.1128/aac.40.5.1157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Zscheck K. K., Hull R., Murray B. E. Restriction mapping and hybridization studies of a beta-lactamase-encoding fragment from Streptococcus (Enterococcus) faecalis. Antimicrob Agents Chemother. 1988 May;32(5):768–769. doi: 10.1128/aac.32.5.768. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Zscheck K. K., Murray B. E. Genes involved in the regulation of beta-lactamase production in enterococci and staphylococci. Antimicrob Agents Chemother. 1993 Sep;37(9):1966–1970. doi: 10.1128/aac.37.9.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Zscheck K. K., Murray B. E. Nucleotide sequence of the beta-lactamase gene from Enterococcus faecalis HH22 and its similarity to staphylococcal beta-lactamase genes. Antimicrob Agents Chemother. 1991 Sep;35(9):1736–1740. doi: 10.1128/aac.35.9.1736. [DOI] [PMC free article] [PubMed] [Google Scholar]

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