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. 1996 Nov;40(11):2648–2650. doi: 10.1128/aac.40.11.2648

Recognition of aminoglycoside antibiotics by enterococcal-staphylococcal aminoglycoside 3'-phosphotransferase type IIIa: role of substrate amino groups.

G A McKay 1, J Roestamadji 1, S Mobashery 1, G D Wright 1
PMCID: PMC163593  PMID: 8913482

Abstract

The interactions of the aminoglycoside 3'-phosphotransferase IIIa with aminoglycoside antibiotics lacking specific amino groups were examined by steady-state kinetic analyses. The results demonstrate that an amino group on C-1 and either an amino or a hydroxyl group at the 2' and 6' positions are important for detoxification of aminoglycosides by this enzyme.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Edson R. S., Terrell C. L. The aminoglycosides. Mayo Clin Proc. 1991 Nov;66(11):1158–1164. doi: 10.1016/s0025-6196(12)65798-x. [DOI] [PubMed] [Google Scholar]
  2. McKay G. A., Thompson P. R., Wright G. D. Broad spectrum aminoglycoside phosphotransferase type III from Enterococcus: overexpression, purification, and substrate specificity. Biochemistry. 1994 Jun 7;33(22):6936–6944. doi: 10.1021/bi00188a024. [DOI] [PubMed] [Google Scholar]
  3. McKay G. A., Wright G. D. Kinetic mechanism of aminoglycoside phosphotransferase type IIIa. Evidence for a Theorell-Chance mechanism. J Biol Chem. 1995 Oct 20;270(42):24686–24692. doi: 10.1074/jbc.270.42.24686. [DOI] [PubMed] [Google Scholar]
  4. Moellering R. C., Jr, Wennersten C., Weinstein A. J. Penicillin-tobramycin synergism against enterococci: a comparison with penicillin and gentamicin. Antimicrob Agents Chemother. 1973 Apr;3(4):526–529. doi: 10.1128/aac.3.4.526. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Murray B. E. The life and times of the Enterococcus. Clin Microbiol Rev. 1990 Jan;3(1):46–65. doi: 10.1128/cmr.3.1.46. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Shaw K. J., Rather P. N., Hare R. S., Miller G. H. Molecular genetics of aminoglycoside resistance genes and familial relationships of the aminoglycoside-modifying enzymes. Microbiol Rev. 1993 Mar;57(1):138–163. doi: 10.1128/mr.57.1.138-163.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Siregar J. J., Lerner S. A., Mobashery S. Purification and characterization of aminoglycoside 3'-phosphotransferase type IIa and kinetic comparison with a new mutant enzyme. Antimicrob Agents Chemother. 1994 Apr;38(4):641–647. doi: 10.1128/aac.38.4.641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Siregar J. J., Miroshnikov K., Mobashery S. Purification, characterization, and investigation of the mechanism of aminoglycoside 3'-phosphotransferase type Ia. Biochemistry. 1995 Oct 3;34(39):12681–12688. doi: 10.1021/bi00039a026. [DOI] [PubMed] [Google Scholar]
  9. Thompson P. R., Hughes D. W., Wright G. D. Regiospecificity of aminoglycoside phosphotransferase from Enterococci and Staphylococci (APH(3')-IIIa). Biochemistry. 1996 Jul 2;35(26):8686–8695. doi: 10.1021/bi960389w. [DOI] [PubMed] [Google Scholar]
  10. Trieu-Cuot P., Courvalin P. Nucleotide sequence of the Streptococcus faecalis plasmid gene encoding the 3'5"-aminoglycoside phosphotransferase type III. Gene. 1983 Sep;23(3):331–341. doi: 10.1016/0378-1119(83)90022-7. [DOI] [PubMed] [Google Scholar]

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