Skip to main content
PMC home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1997 Feb;41(2):468–470. doi: 10.1128/aac.41.2.468

Transferable hyperproduction of TEM-1 beta-lactamase in Shigella flexneri due to a point mutation in the pribnow box.

L K Siu 1, P L Ho 1, K Y Yuen 1, S S Wong 1, P Y Chau 1
PMCID: PMC163732  PMID: 9021210

Abstract

TEM-1 hyperproduction in two ampicillin-sulbactam-resistant Shigella flexneri strains was studied. In both strains the blaTEM gene was encoded as a single copy on a large conjugatively transferable plasmid. A single G-->T transversion at position 1 of the -10 consensus sequence was identified to be the mechanism of TEM-1 hyperproduction.

Full Text

The Full Text of this article is available as a PDF (316.6 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bush K., Jacoby G. A., Medeiros A. A. A functional classification scheme for beta-lactamases and its correlation with molecular structure. Antimicrob Agents Chemother. 1995 Jun;39(6):1211–1233. doi: 10.1128/aac.39.6.1211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chapon C. Expression of malT, the regulator gene of the maltose region in Escherichia coli, is limited both at transcription and translation. EMBO J. 1982;1(3):369–374. doi: 10.1002/j.1460-2075.1982.tb01176.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chen S. T., Clowes R. C. Two improved promoter sequences for the beta-lactamase expression arising from a single base-pair substitution. Nucleic Acids Res. 1984 Apr 11;12(7):3219–3234. doi: 10.1093/nar/12.7.3219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cooksey R. C., Clark N. C., Thornsberry C. A gene probe for TEM type beta-lactamases. Antimicrob Agents Chemother. 1985 Jul;28(1):154–156. doi: 10.1128/aac.28.1.154. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fournier B., Arlet G., Lagrange P. H., Philippon A. Klebsiella oxytoca: resistance to aztreonam by overproduction of the chromosomally encoded beta-lactamase. FEMS Microbiol Lett. 1994 Feb 1;116(1):31–36. doi: 10.1111/j.1574-6968.1994.tb06671.x. [DOI] [PubMed] [Google Scholar]
  6. Fournier B., Lagrange P. H., Philippon A. beta-lactamase gene promoters of 71 clinical strains of Klebsiella oxytoca. Antimicrob Agents Chemother. 1996 Feb;40(2):460–463. doi: 10.1128/aac.40.2.460. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fournier B., Lu C. Y., Lagrange P. H., Krishnamoorthy R., Philippon A. Point mutation in the pribnow box, the molecular basis of beta-lactamase overproduction in Klebsiella oxytoca. Antimicrob Agents Chemother. 1995 Jun;39(6):1365–1368. doi: 10.1128/aac.39.6.1365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Goussard S., Courvalin P. Sequence of the genes blaT-1B and blaT-2. Gene. 1991 Jun 15;102(1):71–73. doi: 10.1016/0378-1119(91)90540-r. [DOI] [PubMed] [Google Scholar]
  9. Hawley D. K., McClure W. R. Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Res. 1983 Apr 25;11(8):2237–2255. doi: 10.1093/nar/11.8.2237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jaurin B., Grundström T., Normark S. Sequence elements determining ampC promoter strength in E. coli. EMBO J. 1982;1(7):875–881. doi: 10.1002/j.1460-2075.1982.tb01263.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kado C. I., Liu S. T. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol. 1981 Mar;145(3):1365–1373. doi: 10.1128/jb.145.3.1365-1373.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Martinez J. L., Cercenado E., Rodriguez-Creixems M., Vincente-Perez M. F., Delgado-Iribarren A., Baquero F. Resistance to beta-lactam/clavulanate. Lancet. 1987 Dec 19;2(8573):1473–1473. doi: 10.1016/s0140-6736(87)91180-9. [DOI] [PubMed] [Google Scholar]
  13. Mathew A., Harris A. M., Marshall M. J., Ross G. W. The use of analytical isoelectric focusing for detection and identification of beta-lactamases. J Gen Microbiol. 1975 May;88(1):169–178. doi: 10.1099/00221287-88-1-169. [DOI] [PubMed] [Google Scholar]
  14. Normark S., Edlund T., Grundström T., Bergström S., Wolf-Watz H. Escherichia coli K-12 mutants hyperproducing chromosomal beta-lactamase by gene repetitions. J Bacteriol. 1977 Dec;132(3):912–922. doi: 10.1128/jb.132.3.912-922.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Seetulsingh P. S., Hall L. M., Livermore D. M. Activity of clavulanate combinations against TEM-1 beta-lactamase-producing Escherichia coli isolates obtained in 1982 and 1989. J Antimicrob Chemother. 1991 Jun;27(6):749–759. doi: 10.1093/jac/27.6.749. [DOI] [PubMed] [Google Scholar]
  16. Stapleton P., Wu P. J., King A., Shannon K., French G., Phillips I. Incidence and mechanisms of resistance to the combination of amoxicillin and clavulanic acid in Escherichia coli. Antimicrob Agents Chemother. 1995 Nov;39(11):2478–2483. doi: 10.1128/aac.39.11.2478. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sutcliffe J. G. Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3737–3741. doi: 10.1073/pnas.75.8.3737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Togna A. P., Shuler M. L., Wilson D. B. Effects of plasmid copy number and runaway plasmid replication on overproduction and excretion of beta-lactamase from Escherichia coli. Biotechnol Prog. 1993 Jan-Feb;9(1):31–39. doi: 10.1021/bp00019a005. [DOI] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES