Abstract
Experiments to grow Tetraselmis chuii (a marine alga) and Daphnia magna in the presence of inorganic arsenate are described. The algae incorporate arsenic rather efficiently and form a lipid-soluble organic arsenic compound. T. chuii has been successfully mass cultured in a medium containing 10 ppm arsenic as arsenate. Daphnia magna was cultured in a medium containing 74As-labeled H3AsO4 and 1 ppm Na2HAsO4 expressed as arsenic. The arsenic metabolites were extracted with a chloroform-methanol solution and isolated by using column and thin-layer chromatography. TLC analysis of the metabolites revealed the presence of a 74As-containing product which migrated with phosphatidylethanolamine. This product was hydrolyzed with the phospholipases A, C, and D. The experimental results are not inconsistent with the presence of an arsenocholine moiety in the lipids. Arsenocholine, arsenobetaine, and acetylarsenocholine have been synthesized and will serve as reference substances in the chromatography experiments. The preparation of arsenocholine-containing lipids is in progress.
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Selected References
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- Cerbon J., Sharpless N. Arsenic-lipid complex formation during sugar transport. Biochim Biophys Acta. 1966 Oct 10;126(2):292–298. doi: 10.1016/0926-6585(66)90066-5. [DOI] [PubMed] [Google Scholar]
- DAVIDSON F. M., LONG C. The structure of the naturally occurring phosphoglycerides. 4. Action of cabbage-leaf phospholipase D on ovolecithin and related substances. Biochem J. 1958 Jul;69(3):458–466. doi: 10.1042/bj0690458. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
- Lunde G. Analysis of arsenic and bromine in marine and terrestrial oils. J Am Oil Chem Soc. 1972 Jan;49(1):44–47. doi: 10.1007/BF02545138. [DOI] [PubMed] [Google Scholar]
- Lunde G. Analysis of arsenic in marine oils by neutron activation. Evidence of arseno organic compounds. J Am Oil Chem Soc. 1968 May;45(5):331–332. doi: 10.1007/BF02667103. [DOI] [PubMed] [Google Scholar]
- Lunde G. Separation and analysis of organic-bound and inorganic arsenic in marine organisms. J Sci Food Agric. 1973 Sep;24(9):1021–1027. doi: 10.1002/jsfa.2740240904. [DOI] [PubMed] [Google Scholar]
- Lunde G. The synthesis of fat and water soluble arseno organic compounds in marine and limnetic algae. Acta Chem Scand. 1973;27(5):1586–1594. doi: 10.3891/acta.chem.scand.27-1586. [DOI] [PubMed] [Google Scholar]
- Lunde G. Water soluble arseno-organic compounds in marine fishes. Nature. 1969 Oct 11;224(5215):186–187. doi: 10.1038/224186b0. [DOI] [PubMed] [Google Scholar]
- Nutter L. J., Privett O. S. Phospholipase a properties of several snake venom preparations. Lipids. 1966 Jul;1(4):258–262. doi: 10.1007/BF02531612. [DOI] [PubMed] [Google Scholar]
- Otnaess A. B., Holm T. The effect of phospholipase C on human blood platelets. J Clin Invest. 1976 Jun;57(6):1419–1425. doi: 10.1172/JCI108411. [DOI] [PMC free article] [PubMed] [Google Scholar]