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. 1979 Aug;31:97–111. doi: 10.1289/ehp.793197

Detection of mitotic and meiotic aneuploidy in the yeast Saccharomyces cerevisiae.

J M Parry, D Sharp, E M Parry
PMCID: PMC1637641  PMID: 387403

Abstract

A number of genetic systems are described which involve the use of the yeast Saccharomyces cerevisiae. The systems may be used to detect the production of aneuploid cells produced during both mitotic and meiotic cell division in the presence of genetically active chemicals. During mitotic cell division, monosomic colonies (2n - 1) may be detected by plating upon selective medium. Increases in such monosomic colonies are produced by exposure of cells to a number of chemical mutagens such as ethyl methane-sulfonate and mitomycin C. More importantly, monosomic colonies are also induced by nonmutagens such as sulfacetamide and saccharin, which suggests that such chemicals are capable of inducing aneuploidy (aneugenic) in the absence of mutagenic activity. Genetic analysis of aneuploid colonies produced on nonselective medium indicate that at least a proportion of the monosomic colonies were the result of mitotic nondisjunction. During meiotic cell division, disomic cells (n + 1) produced by chromosome nondisjunction may be detected by plating on selective media. The frequency of disomic cells has been shown to increase after exposure to p-fluorophenylalanine.

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Selected References

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  1. BROWN G. M. The biosynthesis of folic acid. II. Inhibition by sulfonamides. J Biol Chem. 1962 Feb;237:536–540. [PubMed] [Google Scholar]
  2. Blackburn G. M., Flavell A. J., Thompson M. H. Oxidative and photochemical linkage of diethylstilbestrol to DNA in vitro. Cancer Res. 1974 Aug;34(8):2015–2019. [PubMed] [Google Scholar]
  3. Bruenn J., Mortimer R. K. Isolation of monosomics in yeast. J Bacteriol. 1970 May;102(2):548–551. doi: 10.1128/jb.102.2.548-551.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Campbell D. A., Fogel S., Lusnak K. Mitotic chromosome loss in a disomic haploid of Saccharomyces cerevisiae. Genetics. 1975 Mar;79(3):383–396. doi: 10.1093/genetics/79.3.383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Culbertson M. R., Henry S. A. Genetic analysis of hybrid strains trisomic for the chromosome containing a fatty acid synthetase gene complex (fas1) in yeast. Genetics. 1973 Nov;75(3):441–458. doi: 10.1093/genetics/75.3.441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Davies P. J., Parry J. M. The modification of induced genetic change in yeast by an amino acid analogue. Mol Gen Genet. 1978 Jun 14;162(2):183–190. doi: 10.1007/BF00267875. [DOI] [PubMed] [Google Scholar]
  7. Davis B. K. Genetic analysis of a meiotic mutant resulting in precocious sister-centromere separation in Drosophila melanogaster. Mol Gen Genet. 1971;113(3):251–272. doi: 10.1007/BF00339546. [DOI] [PubMed] [Google Scholar]
  8. Davis D. G. Chromosome Behavior under the Influence of Claret-Nondisjunctional in DROSOPHILA MELANOGASTER. Genetics. 1969 Mar;61(3):577–594. doi: 10.1093/genetics/61.3.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fogel S., Roth R. Mutations affecting meiotic gene conversion in yeast. Mol Gen Genet. 1974 May 31;130(3):189–201. doi: 10.1007/BF00268799. [DOI] [PubMed] [Google Scholar]
  10. Gelbart W. M. A new mutant controlling mitotic chromosome disjunction in Drosophila melanogaster. Genetics. 1974 Jan;76(1):51–63. doi: 10.1093/genetics/76.1.51. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Haber J. E. Bisexual mating behavior in a diploid of Saccharomyces cerevisiae: evidence for genetically controlled non-random chromosome loss during vegetative growth. Genetics. 1974 Nov;78(3):843–858. doi: 10.1093/genetics/78.3.843. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Herbst A. L., Ulfelder H., Poskanzer D. C. Adenocarcinoma of the vagina. Association of maternal stilbestrol therapy with tumor appearance in young women. N Engl J Med. 1971 Apr 15;284(15):878–881. doi: 10.1056/NEJM197104222841604. [DOI] [PubMed] [Google Scholar]
  13. JACOBS P. A., BRUNTON M., COURT BROWN W. M., DOLL R., GOLDSTEIN H. Change of human chromosome count distribution with age: evidence for a sex differences. Nature. 1963 Mar 16;197:1080–1081. doi: 10.1038/1971080a0. [DOI] [PubMed] [Google Scholar]
  14. JACOBS P. A., COURT BROWN W. M., DOLL R. Distribution of human chromosome counts in relation to age. Nature. 1961 Sep 16;191:1178–1180. doi: 10.1038/1911178a0. [DOI] [PubMed] [Google Scholar]
  15. Kaback D. B., Bhargava M. M., Halvorson H. O. Letter: Location and arrangement of genes coding for ribosomal RNA in Saccharomyces cerevisiae. J Mol Biol. 1973 Oct 5;79(4):735–739. doi: 10.1016/0022-2836(73)90076-4. [DOI] [PubMed] [Google Scholar]
  16. LHOAS P. Mitotic haploidization by treatment of Aspergillus niger diploids with para-fluorophenylalanine. Nature. 1961 May 20;190:744–744. doi: 10.1038/190744a0. [DOI] [PubMed] [Google Scholar]
  17. Liras P., McCusker J., Mascioli S., Haber J. E. Characterization of a mutation in yeast causing nonrandom chromosome loss during mitosis. Genetics. 1978 Apr;88(4 Pt 1):651–671. [PMC free article] [PubMed] [Google Scholar]
  18. Mason J. M. Orientation disruptor (ord): a recombination-defective and disjunction-defective meiotic mutant in Drosophila melanogaster. Genetics. 1976 Nov;84(3):545–572. doi: 10.1093/genetics/84.3.545. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Mortimer R. K., Hawthorne D. C. Genetic Mapping in Saccharomyces IV. Mapping of Temperature-Sensitive Genes and Use of Disomic Strains in Localizing Genes. Genetics. 1973 May;74(1):33–54. doi: 10.1093/genetics/74.1.33. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Munro I. C., Moodie C. A., Krewski D., Grice H. C. A carcinogenicity study of commercial saccharin in the rat. Toxicol Appl Pharmacol. 1975 Jun;32(3):513–526. doi: 10.1016/0041-008x(75)90116-7. [DOI] [PubMed] [Google Scholar]
  21. Parry D. M. A meiotic mutant affecting recombination in female Drosophila melanogaster. Genetics. 1973 Mar;73(3):465–486. doi: 10.1093/genetics/73.3.465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Parry E. M., Cox B. S. The tolerance of aneuploidy in yeast. Genet Res. 1970 Dec;16(3):333–340. doi: 10.1017/s0016672300002597. [DOI] [PubMed] [Google Scholar]
  23. Parry J. M. The use of yeast cultures for the detection of environmental mutagens using a fluctuation test. Mutat Res. 1977 Jun;46(3):165–175. doi: 10.1016/0165-1161(77)90023-1. [DOI] [PubMed] [Google Scholar]
  24. Parry J. M., Zimmerman F. K. The detection of monosomic colonies produced by mitotic chromosome non-disjunction in the yeast Saccharomyces cerevisiae. Mutat Res. 1976 Jul;36(1):49–66. doi: 10.1016/0027-5107(76)90020-8. [DOI] [PubMed] [Google Scholar]
  25. Rodarte-Ramón U. S., Mortimer R. K. Radiation-induced recombination in Saccharomyces: isolation and genetic study of recombination-deficient mutants. Radiat Res. 1972 Jan;49(1):133–147. [PubMed] [Google Scholar]
  26. Roth R., Fogel S. A system selective for yeast mutants deficient in meiotic recombination. Mol Gen Genet. 1971;112(4):295–305. doi: 10.1007/BF00334431. [DOI] [PubMed] [Google Scholar]
  27. Shaffer B., Brearley I., Littlewood R., Fink G. R. A stable aneuploid of Saccharomyces cerevisiae. Genetics. 1971 Apr;67(4):483–495. doi: 10.1093/genetics/67.4.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Øyen T. B. Chromosome I as a possible site for some rRNA cistrons in Saccharomyces cerevisiae. FEBS Lett. 1973 Feb 15;30(1):53–56. doi: 10.1016/0014-5793(73)80617-9. [DOI] [PubMed] [Google Scholar]

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