Abstract
We have developed a rapid PCR-oligonucleotide ligation assay that can discriminate single base substitutions that are associated with clarithromycin resistance in Helicobacter pylori. Susceptible isolates were wild type at positions 2143 and 2144 (cognate to 2058 and 2059 in Escherichia coli), while 93% of the resistant isolates contained A-to-G mutations at either position and 7% of the isolates contained A-to-C mutations at position 2143. In addition, the MIC for 86% of the resistant isolates with an A2143 mutation was > or = 64 micrograms per ml, and that for 89% of the resistant isolates with an A2144 mutation was < or = 32 micrograms per ml.
Full Text
The Full Text of this article is available as a PDF (64.7 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bukanov N. O., Berg D. E. Ordered cosmid library and high-resolution physical-genetic map of Helicobacter pylori strain NCTC11638. Mol Microbiol. 1994 Feb;11(3):509–523. doi: 10.1111/j.1365-2958.1994.tb00332.x. [DOI] [PubMed] [Google Scholar]
- Debets-Ossenkopp Y. J., Sparrius M., Kusters J. G., Kolkman J. J., Vandenbroucke-Grauls C. M. Mechanism of clarithromycin resistance in clinical isolates of Helicobacter pylori. FEMS Microbiol Lett. 1996 Aug 15;142(1):37–42. doi: 10.1111/j.1574-6968.1996.tb08404.x. [DOI] [PubMed] [Google Scholar]
- Fujimoto S., Marshall B., Blaser M. J. PCR-based restriction fragment length polymorphism typing of Helicobacter pylori. J Clin Microbiol. 1994 Feb;32(2):331–334. doi: 10.1128/jcm.32.2.331-334.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goldman R. C., Zakula D., Flamm R., Beyer J., Capobianco J. Tight binding of clarithromycin, its 14-(R)-hydroxy metabolite, and erythromycin to Helicobacter pylori ribosomes. Antimicrob Agents Chemother. 1994 Jul;38(7):1496–1500. doi: 10.1128/aac.38.7.1496. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jiang Q., Hiratsuka K., Taylor D. E. Variability of gene order in different Helicobacter pylori strains contributes to genome diversity. Mol Microbiol. 1996 May;20(4):833–842. doi: 10.1111/j.1365-2958.1996.tb02521.x. [DOI] [PubMed] [Google Scholar]
- Lucier T. S., Heitzman K., Liu S. K., Hu P. C. Transition mutations in the 23S rRNA of erythromycin-resistant isolates of Mycoplasma pneumoniae. Antimicrob Agents Chemother. 1995 Dec;39(12):2770–2773. doi: 10.1128/aac.39.12.2770. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meier A., Kirschner P., Springer B., Steingrube V. A., Brown B. A., Wallace R. J., Jr, Böttger E. C. Identification of mutations in 23S rRNA gene of clarithromycin-resistant Mycobacterium intracellulare. Antimicrob Agents Chemother. 1994 Feb;38(2):381–384. doi: 10.1128/aac.38.2.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moazed D., Noller H. F. Chloramphenicol, erythromycin, carbomycin and vernamycin B protect overlapping sites in the peptidyl transferase region of 23S ribosomal RNA. Biochimie. 1987 Aug;69(8):879–884. doi: 10.1016/0300-9084(87)90215-x. [DOI] [PubMed] [Google Scholar]
- Nash K. A., Inderlied C. B. Genetic basis of macrolide resistance in Mycobacterium avium isolated from patients with disseminated disease. Antimicrob Agents Chemother. 1995 Dec;39(12):2625–2630. doi: 10.1128/aac.39.12.2625. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nickerson D. A., Kaiser R., Lappin S., Stewart J., Hood L., Landegren U. Automated DNA diagnostics using an ELISA-based oligonucleotide ligation assay. Proc Natl Acad Sci U S A. 1990 Nov;87(22):8923–8927. doi: 10.1073/pnas.87.22.8923. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sigmund C. D., Ettayebi M., Borden A., Morgan E. A. Antibiotic resistance mutations in ribosomal RNA genes of Escherichia coli. Methods Enzymol. 1988;164:673–690. doi: 10.1016/s0076-6879(88)64077-8. [DOI] [PubMed] [Google Scholar]
- Stone G. G., Shortridge D., Flamm R. K., Versalovic J., Beyer J., Idler K., Zulawinski L., Tanaka S. K. Identification of a 23S rRNA gene mutation in clarithromycin-resistant Helicobacter pylori. Helicobacter. 1996 Dec;1(4):227–228. doi: 10.1111/j.1523-5378.1996.tb00043.x. [DOI] [PubMed] [Google Scholar]
- Versalovic J., Shortridge D., Kibler K., Griffy M. V., Beyer J., Flamm R. K., Tanaka S. K., Graham D. Y., Go M. F. Mutations in 23S rRNA are associated with clarithromycin resistance in Helicobacter pylori. Antimicrob Agents Chemother. 1996 Feb;40(2):477–480. doi: 10.1128/aac.40.2.477. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vester B., Garrett R. A. A plasmid-coded and site-directed mutation in Escherichia coli 23S RNA that confers resistance to erythromycin: implications for the mechanism of action of erythromycin. Biochimie. 1987 Aug;69(8):891–900. doi: 10.1016/0300-9084(87)90217-3. [DOI] [PubMed] [Google Scholar]