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. 2000 Apr;108(4):329–332. doi: 10.1289/ehp.00108329

Embryonic treatment with xenobiotics disrupts steroid hormone profiles in hatchling red-eared slider turtles (Trachemys scripta elegans).

E Willingham 1, T Rhen 1, J T Sakata 1, D Crews 1
PMCID: PMC1638010  PMID: 10753091

Abstract

Many compounds in the environment capable of acting as endocrine disruptors have been assayed for their developmental effects on morphogenesis; however, few studies have addressed how such xenobiotics affect physiology. In the current study we examine the effects of three endocrine-disrupting compounds, chlordane, trans-nonachlor, and the polychlorinated biphenyl (PCB) mixture Aroclor 1242, on the steroid hormone concentrations of red-eared slider turtle (Trachemys scripta elegans) hatchlings treated in ovo. Basal steroid concentrations and steroid concentrations in response to follicle-stimulating hormone were examined in both male and female turtles treated with each of the three compounds. Treated male turtles exposed to Aroclor 1242 or chlordane exhibited significantly lower testosterone concentrations than controls, whereas chlordane-treated females had significantly lower progesterone, testosterone, and 5[alpha]-dihydrotestosterone concentrations relative to controls. The effects of these endocrine disruptors extend beyond embryonic development, altering sex-steroid physiology in exposed animals.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bergeron J. M., Crews D., McLachlan J. A. PCBs as environmental estrogens: turtle sex determination as a biomarker of environmental contamination. Environ Health Perspect. 1994 Sep;102(9):780–781. doi: 10.1289/ehp.94102780. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cooke G. M., Price C. A., Oko R. J. Effects of in utero and lactational exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on serum androgens and steroidogenic enzyme activities in the male rat reproductive tract. J Steroid Biochem Mol Biol. 1998 Nov;67(4):347–354. doi: 10.1016/s0960-0760(98)00127-7. [DOI] [PubMed] [Google Scholar]
  3. Crain D. A., Guillette L. J., Jr, Rooney A. A., Pickford D. B. Alterations in steroidogenesis in alligators (Alligator mississippiensis) exposed naturally and experimentally to environmental contaminants. Environ Health Perspect. 1997 May;105(5):528–533. doi: 10.1289/ehp.97105528. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Crews D., Bergeron J. M., Bull J. J., Flores D., Tousignant A., Skipper J. K., Wibbels T. Temperature-dependent sex determination in reptiles: proximate mechanisms, ultimate outcomes, and practical applications. Dev Genet. 1994;15(3):297–312. doi: 10.1002/dvg.1020150310. [DOI] [PubMed] [Google Scholar]
  5. Crews D., Bergeron J. M. Role of reductase and aromatase in sex determination in the red-eared slider (Trachemys scripta), a turtle with temperature-dependent sex determination. J Endocrinol. 1994 Nov;143(2):279–289. doi: 10.1677/joe.0.1430279. [DOI] [PubMed] [Google Scholar]
  6. Crews D. Temperature-dependent sex determination: the interplay of steroid hormones and temperature. Zoolog Sci. 1996 Feb;13(1):1–13. doi: 10.2108/zsj.13.1. [DOI] [PubMed] [Google Scholar]
  7. Crisp T. M., Clegg E. D., Cooper R. L., Wood W. P., Anderson D. G., Baetcke K. P., Hoffmann J. L., Morrow M. S., Rodier D. J., Schaeffer J. E. Environmental endocrine disruption: an effects assessment and analysis. Environ Health Perspect. 1998 Feb;106 (Suppl 1):11–56. doi: 10.1289/ehp.98106s111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Orlando E. F., Denslow N. D., Folmar L. C., Guillette L. J., Jr A comparison of the reproductive physiology of largemouth bass, Micropterus salmoides, collected from the Escambia and Blackwater Rivers in Florida. Environ Health Perspect. 1999 Mar;107(3):199–204. doi: 10.1289/ehp.99107199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rhen T., Willingham E., Sakata J. T., Crews D. Incubation temperature influences sex-steroid levels in juvenile red-eared slider turtles, Trachemys scripta, a species with temperature-dependent sex determination. Biol Reprod. 1999 Nov;61(5):1275–1280. doi: 10.1095/biolreprod61.5.1275. [DOI] [PubMed] [Google Scholar]
  10. Willingham E., Crews D. Sex reversal effects of environmentally relevant xenobiotic concentrations on the red-eared slider turtle, a species with temperature-dependent sex determination. Gen Comp Endocrinol. 1999 Mar;113(3):429–435. doi: 10.1006/gcen.1998.7221. [DOI] [PubMed] [Google Scholar]

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